Abstract
Enterotoxigenic Staphylococcus aureus strains were grown with a non-enterotoxigenic strain in laboratory medium, in milk, and in ham. Differences in pigmentation were used to differentiate the enterotoxigenic strains from the non-enterotoxigenic ones. Enterotoxin was detectable in milk when the colony counts of the non-enterotoxigenic strain were 15 to 20 times greater than those of the enterotoxigenic ones and in ham when the ratio was 60 to 77:1. Enterotoxin was detectable in milk when the enterotoxigenic strains reached counts of 10(7) colony-forming units per ml and in ham when the counts reached 10(8) colony-forming units per ml. It may be necessary in some food poisoning outbreaks to examine many isolates (up to 50 or 60) for enterotoxin production to be able to detect the enterotoxigenic staphylococci.
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Selected References
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- Donnelly C. B., Leslie J. E., Black L. A. Production of enterotoxin A in milk. Appl Microbiol. 1968 Jun;16(6):917–924. doi: 10.1128/am.16.6.917-924.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kato E., Khan M., Kujovich L., Bergdoll M. S. Production of enterotoxin a. Appl Microbiol. 1966 Nov;14(6):966–972. doi: 10.1128/am.14.6.966-972.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller B. A., Reiser R. F., Bergdoll M. S. Detection of staphylococcal enterotoxins A, B, C, D, and E in foods by radioimnunoassay, using staphyloccal cells containing protein A as immunoadsorbent. Appl Environ Microbiol. 1978 Sep;36(3):421–426. doi: 10.1128/aem.36.3.421-426.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reiser R., Conaway D., Bergdoll M. S. Detection of staphylococcal enterotoxin in foods. Appl Microbiol. 1974 Jan;27(1):83–85. doi: 10.1128/am.27.1.83-85.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robbins R., Gould S., Bergdoll M. Detecting the enterotoxigenicity of Staphylococcus aureus strains. Appl Microbiol. 1974 Dec;28(6):946–950. doi: 10.1128/am.28.6.946-950.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tatini S. R., Jezeski J. J., Olson J. C., Jr, Casman E. P. Factors influencing the production of staphylococcal enterotoxin A in milk. J Dairy Sci. 1971 Mar;54(3):312–320. doi: 10.3168/jds.S0022-0302(71)85835-6. [DOI] [PubMed] [Google Scholar]