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. 1980 Nov;40(5):897–900. doi: 10.1128/aem.40.5.897-900.1980

Rocket Immunoelectrophoresis of the Entomocidal Parasporal Crystal of Bacillus thuringiensis subsp. kurstaki†

R E Andrews Jr 1,2, J J Iandolo 2, B S Campbell 1, L I Davidson 1, L A Bulla Jr 1,2
PMCID: PMC291686  PMID: 16345656

Abstract

Rocket immunoelectrophoresis was used to quantitate the soluble parasporal crystal of Bacillus thuringiensis subsp. kurstaki. The method described is rapid, reliable, specific, and extremely accurate, and it can be used to measure crystal toxin in commercial microbial insecticides that contain a mixture of spores, vegetative cells, and carrier materials.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews R. E., Jr, Spence K. D., Miller L. K. Virulence of cloned variants of Autographa californica nuclear polyhedrosis virus. Appl Environ Microbiol. 1980 Apr;39(4):932–933. doi: 10.1128/aem.39.4.932-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bulla L. A., Jr, Davidson L. I., Kramer K. J., Jones B. L. Purification of the insecticidal toxin from the parasporal crystal of Bacillus thuringiensis subsp. kurstaki. Biochem Biophys Res Commun. 1979 Dec 14;91(3):1123–1130. doi: 10.1016/0006-291x(79)91997-1. [DOI] [PubMed] [Google Scholar]
  3. Bulla L. A., Jr, Kramer K. J., Davidson L. I. Characterization of the entomocidal parasporal crystal of Bacillus thuringiensis. J Bacteriol. 1977 Apr;130(1):375–383. doi: 10.1128/jb.130.1.375-383.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dulmage H. T. Production of the spore-delta-endotoxin complex by variants of bacillus thuringiensis in two fermentation media. J Invertebr Pathol. 1970 Nov;16(3):385–389. doi: 10.1016/0022-2011(70)90157-6. [DOI] [PubMed] [Google Scholar]
  5. Iandolo J. J., Shafer W. M. Regulation of staphylococcal enterotoxin B. Infect Immun. 1977 May;16(2):610–616. doi: 10.1128/iai.16.2.610-616.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Schesser J. H., Kramer K. J., Bulla L. A., Jr Bioassay for homogeneous parasporal crystal of Bacillus thuringiensis using the tobacco hornworm, Manduca sexta. Appl Environ Microbiol. 1977 Apr;33(4):878–880. doi: 10.1128/aem.33.4.878-880.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sharpe E. S., Nickerson K. W., Bulla L. A., Jr, Aronson J. N. Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents. Appl Microbiol. 1975 Dec;30(6):1052–1053. doi: 10.1128/am.30.6.1052-1053.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stahly D. P., Dingman D. W., Bulla L. A., Jr, Aronson A. I. Possible origin and function of the parasporal crystal in Bacillus thuringiensis. Biochem Biophys Res Commun. 1978 Oct 16;84(3):581–588. doi: 10.1016/0006-291x(78)90745-3. [DOI] [PubMed] [Google Scholar]

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