Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1971 Jan;50(1):112–118. doi: 10.1172/JCI106464

Depression of proximal tubular sodium reabsorption in the dog in response to renal beta adrenergic stimulation by isoproterenol

John R Gill Jr 1, Alfred G T Casper 1
PMCID: PMC291898  PMID: 4395909

Abstract

Water diuresis was produced in anesthetized hypophysectomized, cortisone-treated dogs by infusion of 2.5% dextrose. Alpha adrenergic blockade of the left kidney produced by infusion of phenoxybenzamine in the left renal artery was associated with a significantly (P < 0.05) greater rate of urine flow (V) and free water excretion (CH2O) in the left kidney than in the right despite similar glomerular filtration rates (GFR) (17 ± 1.3 ml/min, left; 18 ±0.9 ml/min, right). Sodium excretion (UNaV) was similar in the two kidneys (3 and 5 μEq/min).

When beta adrenergic stimulation of the left kidney was superimposed on alpha blockade by the addition of isoproterenol to the left renal artery infusate, GFR remained unchanged and similar in the two kidneys, as V and CH2O increased significantly (P < 0.01) in the left kidney but not in the right. When isoproterenol was discontinued, V and CH2O returned towards control in the left kidney and remained unchanged in the right. The ratios of the left kidney to the right during control, isoproterenol, and postcontrol were 1.22, 1.65, and 1.35, respectively, for V and 1.36, 1.90, and 1.44, respectively, for CH2O. Sodium excretion remained unchanged and similar in the two kidneys throughout the study.

The results indicate that blockade of alpha adrenergic activity inhibits the increased proximal tubular sodium reabsorption which anesthesia induces in the dog.

Beta adrenergic stimulation appears to decrease proximal tubular sodium reabsorption but does not prevent virtually complete reabsorption of the increased quantity of delivered sodium by the ascending limb of the loop of Henle and the distal tubule. These changes in sodium reabsorption presumably are not associated with changes in colloid osmotic pressure or hydrostatic pressure in the peritubular capillary inasmuch as cortical and non-cortical plasma flow, filtration fraction, and mean arterial pressure in the left kidney were unchanged. Thus, isoproterenol probably produced its effects through a direct action on the renal tubule, possibly through the mediation of the adenyl cyclase system.

Full text

PDF
113

Images in this article

115Image
on p.115

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNE R. M. Hemodynamics and sodium excretion of denervated kidney in anesthetized and unanesthetized dog. Am J Physiol. 1952 Oct;171(1):148–158. doi: 10.1152/ajplegacy.1952.171.1.148. [DOI] [PubMed] [Google Scholar]
  2. Brenner B. M., Falchuk K. H., Keimowitz R. I., Berliner R. W. The relationship between peritubular capillary protein concentration and fluid reabsorption by the renal proximal tubule. J Clin Invest. 1969 Aug;48(8):1519–1531. doi: 10.1172/JCI106118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chase L. R., Aurbach G. D. Renal adenyl cyclase: anatomically separate sites for parathyroid hormone and vasopressin. Science. 1968 Feb 2;159(3814):545–547. doi: 10.1126/science.159.3814.545. [DOI] [PubMed] [Google Scholar]
  4. Eknoyan G., Suki W. N., Rector F. C., Jr, Seldin D. W. Functional characteristics of the diluting segment of the dog nephron and the effect of extracellular volume expansion on its reabsorptive capacity. J Clin Invest. 1967 Jul;46(7):1178–1188. doi: 10.1172/JCI105611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gill J. R., Jr, Carr A. A., Fleischmann L. E., Casper A. G., Bartter F. C. Effects of pentolinium on sodium excretion in dogs with constriction of the vena cava. Am J Physiol. 1967 Jan;212(1):191–196. doi: 10.1152/ajplegacy.1967.212.1.191. [DOI] [PubMed] [Google Scholar]
  6. Gill J. R., Jr, Casper A. G. Role of the sympathetic nervous system in the renal response to hemorrhage. J Clin Invest. 1969 May;48(5):915–922. doi: 10.1172/JCI106050. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Handler J. S., Bensinger R., Orloff J. Effect of adrenergic agents on toad bladder response to ADH, 3',5'-AMP, and theophylline. Am J Physiol. 1968 Nov;215(5):1024–1031. doi: 10.1152/ajplegacy.1968.215.5.1024. [DOI] [PubMed] [Google Scholar]
  8. Knox F. G., Howards S. S., Wright F. S., Davis B. B., Berliner R. W. Effect of dilution and expansion of blood volume on proximal sodium reabsorption. Am J Physiol. 1968 Nov;215(5):1041–1048. doi: 10.1152/ajplegacy.1968.215.5.1041. [DOI] [PubMed] [Google Scholar]
  9. Mark A. L., Eckstein J. W., Abboud F. M., Wendling M. G. Renal vascular responses to isoproterenol. Am J Physiol. 1969 Sep;217(3):764–767. doi: 10.1152/ajplegacy.1969.217.3.764. [DOI] [PubMed] [Google Scholar]
  10. Martino J. A., Earley L. E. Demonstraton of a role of physical factors as determinants of the natriuretic response to volume expansion. J Clin Invest. 1967 Dec;46(12):1963–1978. doi: 10.1172/JCI105686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martino J. A., Earley L. E. Relationship between intrarenal hydrostatic pressure and hemodynamically induced changes in sodium excretion. Circ Res. 1968 Sep;23(3):371–386. doi: 10.1161/01.res.23.3.371. [DOI] [PubMed] [Google Scholar]
  12. McNay J. L., Goldberg L. I. Comparison of the effects of dopamine, isoproterenol, norepinephrine and bradykinin on canine renal and femoral blood flow. J Pharmacol Exp Ther. 1966 Jan;151(1):23–31. [PubMed] [Google Scholar]
  13. PILKINGTON L. A., BINDER R., DEHAAS J. C., PITTS R. F. INTRARENAL DISTRIBUTION OF BLOOD FLOW. Am J Physiol. 1965 Jun;208:1107–1113. doi: 10.1152/ajplegacy.1965.208.6.1107. [DOI] [PubMed] [Google Scholar]
  14. Pearson J. E., Williams R. L. Analysis of direct renal actions of alpha and beta adrenergic stimulation upon sodium excretion compared to acetylcholine. Br J Pharmacol Chemother. 1968 Jun;33(2):223–241. doi: 10.1111/j.1476-5381.1968.tb00984.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pomeranz B. H., Birtch A. G., Barger A. C. Neural contrfl of intrarenal blood flow. Am J Physiol. 1968 Nov;215(5):1067–1081. doi: 10.1152/ajplegacy.1968.215.5.1067. [DOI] [PubMed] [Google Scholar]
  16. Robison G. A., Butcher R. W., Sutherland E. W. Cyclic AMP. Annu Rev Biochem. 1968;37:149–174. doi: 10.1146/annurev.bi.37.070168.001053. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES