Abstract
Inflow of preformed ammonia in arterial blood, renal production of ammonia, outflow of ammonia in renal venous blood, and urinary excretion of ammonia were measured during the infusion of 15NH4Cl into one renal artery of dogs with chronic metabolic acidosis. Our results show that the specific activity of ammonia measured in the urine and that calculated in the renal pool agree within 95%. Pool specific activity is obtained by dividing the rate of infusion of isotope by the pool turnover rate, i.e., the sum of the rate of ammonia output in the urine and that in renal venous blood. An average of 35% of urinary ammonia is derived from arterial ammonia in these experiments.
We conclude that ammonia is distributed evenly throughout all phases of the kidney within a period less than the transit time of blood through the kidney. Furthermore, from the proportion of urinary ammonia we found to be derived from preformed arterial ammonia (35%), and from our previous demonstration that 73% of urinary ammonia derives from deamidation and/or deamination of plasma glutamine, alanine, glycine, and glutamate, we can account for all of the ammonia that leaves the kidney in renal venous blood and in urine.
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Selected References
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- BALAGURA S., PITTS R. F. Excretion of ammonia injected into renal artery. Am J Physiol. 1962 Jul;203:11–14. doi: 10.1152/ajplegacy.1962.203.1.11. [DOI] [PubMed] [Google Scholar]
- DENIS G., PREUSS H., PITTS R. THE PNH3 OF RENAL TUBULAR CELLS. J Clin Invest. 1964 Apr;43:571–582. doi: 10.1172/JCI104942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fulgraff G., Pitts R. F. Kinetics of ammonia production and excretion in the acidotic dog. Am J Physiol. 1965 Dec;209(6):1206–1212. doi: 10.1152/ajplegacy.1965.209.6.1206. [DOI] [PubMed] [Google Scholar]
- MILNE M. D., SCRIBNER B. H., CRAWFORD M. A. Non-ionic diffusion and the excretion of weak acids and bases. Am J Med. 1958 May;24(5):709–729. doi: 10.1016/0002-9343(58)90376-0. [DOI] [PubMed] [Google Scholar]
- Mondzac A., Ehrlich G. E., Seegmiller J. E. An enzymatic determination of ammonia in biological fluids. J Lab Clin Med. 1965 Sep;66(3):526–531. [PubMed] [Google Scholar]
- ORLOFF J., BERLINER R. W. The mechanism of the excretion of ammonia in the dog. J Clin Invest. 1956 Feb;35(2):223–235. doi: 10.1172/JCI103267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PITTS R. F., PILKINGTON L. A., DEHAAS J. C. N15 TRACER STUDIES ON THE ORIGIN OF URINARY AMMONIA IN THE ACIDOTIC DOG, WITH NOTES ON THE ENZYMATIC SYNTHESIS OF LABELED CLUTAMIC ACID AND GLUTAMINES. J Clin Invest. 1965 May;44:731–745. doi: 10.1172/JCI105186. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PITTS R. F. RENAL PRODUCTION AND EXCRETION OF AMMONIA. Am J Med. 1964 May;36:720–742. doi: 10.1016/0002-9343(64)90182-2. [DOI] [PubMed] [Google Scholar]
- Pitts R. F., Stone W. J. Renal metabolism of alanine. J Clin Invest. 1967 Apr;46(4):530–538. doi: 10.1172/JCI105554. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pitts R. F. The renal metabolism of ammonia. Physiologist. 1966 May;9(2):97–109. [PubMed] [Google Scholar]
- ROBINSON R. R., OWEN E. E. INTRARENAL DISTRIBUTION OF AMMONIA DURING DIURESIS AND ANTIDIURESIS. Am J Physiol. 1965 Jun;208:1129–1134. doi: 10.1152/ajplegacy.1965.208.6.1129. [DOI] [PubMed] [Google Scholar]
- SULLIVAN L. P., McVAUGH M. Effect of rapid and transitory changes in blood and urine pH on NH4 excretion. Am J Physiol. 1963 Jun;204:1077–1085. doi: 10.1152/ajplegacy.1963.204.6.1077. [DOI] [PubMed] [Google Scholar]
- WEINER I. M., MUDGE G. H. RENAL TUBULAR MECHANISMS FOR EXCRETION OF ORGANIC ACIDS AND BASES. Am J Med. 1964 May;36:743–762. doi: 10.1016/0002-9343(64)90183-4. [DOI] [PubMed] [Google Scholar]