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. 1980 Jan;141(1):20–25. doi: 10.1128/jb.141.1.20-25.1980

Physiological control of phosphorylation ribosomal protein S6 in Mucor racemosus.

A Larsen, P S Sypherd
PMCID: PMC293523  PMID: 7353998

Abstract

The level of phosphorylation of ribosomal protein S6 increased with accelerating rates of growth and protein synthesis in Mucor racemosus. Lowered levels of phosphorylation were seen under conditions of metabolic shift-down or the onset of stationary phase, and no phosphorylation was detected in sporangiospores. Changing metabolic states, changing intracellular levels of adenosine triphosphatase, and the level of phosphorylation of protein S6 were correlated in M. racemosus.

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Selected References

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  1. Alberghina F. A., Sturani E., Gohlke J. R. Levels and rates of synthesis of ribosomal ribonucleic acid, transfer ribonucleic acid, and protein in Neurospora crassa in different steady states of growth. J Biol Chem. 1975 Jun 25;250(12):4381–4388. [PubMed] [Google Scholar]
  2. Blat C., Loeb J. E. Effect of glucagon on phosphorylation of some rat liver ribosomal proteins in vivo. FEBS Lett. 1971 Oct 15;18(1):124–126. doi: 10.1016/0014-5793(71)80425-8. [DOI] [PubMed] [Google Scholar]
  3. Boehlke K. W., Friesen J. D. Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of exponential growth rate: calculation of the apparent peptide chain elongation rate. J Bacteriol. 1975 Feb;121(2):429–433. doi: 10.1128/jb.121.2.429-433.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cannon M., Schindler D., Davies J. Methylation of proteins in 60S ribosomal subunits from Saccharomyces cerevisiae. FEBS Lett. 1977 Mar 15;75(1):187–191. doi: 10.1016/0014-5793(77)80083-5. [DOI] [PubMed] [Google Scholar]
  5. Chang F. N., Navickas I. J., Chang C. N., Dancis B. M. Methylation of ribosomal proteins in HeLa cells. Arch Biochem Biophys. 1976 Feb;172(2):627–633. doi: 10.1016/0003-9861(76)90117-x. [DOI] [PubMed] [Google Scholar]
  6. Correze C., Pinell P., Nunez J. Effects of thyroid hormones on phosphorylation of liver ribosomal proteins and on protein phosphokinase activity. FEBS Lett. 1972 Jun 1;23(1):87–91. doi: 10.1016/0014-5793(72)80291-6. [DOI] [PubMed] [Google Scholar]
  7. Gressner A. M., Wool I. G. Influence of glucagon and cyclic adenosine 3':5'-monophosphate on the phosphorylation of rat liver ribosomal protein S6. J Biol Chem. 1976 Mar 10;251(5):1500–1504. [PubMed] [Google Scholar]
  8. Gressner A. M., Wool I. G. The phosphorylation of liver ribosomal proteins in vivo. Evidence that only a single small subunit protein (S6) is phosphorylated. J Biol Chem. 1974 Nov 10;249(21):6917–6925. [PubMed] [Google Scholar]
  9. Gressner A. M., Wool I. G. The stimulation of the phosphorylation of ribosomal protein S6 by cycloheximide and puromycin. Biochem Biophys Res Commun. 1974 Oct 23;60(4):1482–1490. doi: 10.1016/0006-291x(74)90365-9. [DOI] [PubMed] [Google Scholar]
  10. Inderlied C. B., Sypherd P. S. Glucose metabolism and dimorphism in Mucor. J Bacteriol. 1978 Mar;133(3):1282–1286. doi: 10.1128/jb.133.3.1282-1286.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kabat D. Turnover of phosphoryl groups in reticulocyte ribosomal phosphoproteins. J Biol Chem. 1972 Sep 10;247(17):5338–5344. [PubMed] [Google Scholar]
  12. Kaerlein M., Horak I. Phosphorylation of ribosomal proteins in HeLa cells infected with vaccinia virus. Nature. 1976 Jan 15;259(5539):150–151. doi: 10.1038/259150a0. [DOI] [PubMed] [Google Scholar]
  13. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1276–1282. doi: 10.1073/pnas.67.3.1276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Larsen A., Sypherd P. Ribosomal proteins of the dimorphic fungus, Mucor racemosus. Mol Gen Genet. 1979 Aug;175(1):99–109. doi: 10.1007/BF00267861. [DOI] [PubMed] [Google Scholar]
  15. Lastick S. M., Nielsen P. J., McConkey E. H. Phosphorylation of ribosomal protein S6 in suspension cultured HeLa cells. Mol Gen Genet. 1977 Apr 29;152(3):223–230. doi: 10.1007/BF00693074. [DOI] [PubMed] [Google Scholar]
  16. Liew C. C., Gornall A. G. Acetylation of ribosomal proteins. I. Characterization and properties of rat liver ribosomal proteins. J Biol Chem. 1973 Feb 10;248(3):977–983. [PubMed] [Google Scholar]
  17. Orlowski M., Sypherd P. S. Regulation of translation rate during morphogenesis in the fungus Mucor. Biochemistry. 1978 Feb 21;17(4):569–575. doi: 10.1021/bi00597a002. [DOI] [PubMed] [Google Scholar]
  18. Paznokas J. L., Sypherd P. S. Respiratory capacity, cyclic adenosine 3',5'-monophosphate, and morphogenesis of Mucor racemosus. J Bacteriol. 1975 Oct;124(1):134–139. doi: 10.1128/jb.124.1.134-139.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rankine A. D., Leader D. P., Coia A. A. The phosphorylation of the ribosomal proteins of Krebs II ascites cells. Biochim Biophys Acta. 1977 Jan 20;474(2):293–307. doi: 10.1016/0005-2787(77)90203-9. [DOI] [PubMed] [Google Scholar]
  20. Stanley P. E., Williams S. G. Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme. Anal Biochem. 1969 Jun;29(3):381–392. doi: 10.1016/0003-2697(69)90323-6. [DOI] [PubMed] [Google Scholar]
  21. Traugh J. A., Porter G. G. A comparison of ribosomal proteins from rabbit reticulocytes phosphorylated in situ and in vitro. Biochemistry. 1976 Feb 10;15(3):610–616. doi: 10.1021/bi00648a025. [DOI] [PubMed] [Google Scholar]
  22. Walton G. M., Gill G. N., Abrass I. B., Garren L. D. Phosphorylation of ribosome-associated protein by an adenosine 3':5'-cyclic monophosphate-dependent protein kinase: location of the microsomal receptor and protein kinase. Proc Natl Acad Sci U S A. 1971 May;68(5):880–884. doi: 10.1073/pnas.68.5.880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zinker S., Warner J. R. The ribosomal proteins of Saccharomyces cerevisiae. Phosphorylated and exchangeable proteins. J Biol Chem. 1976 Mar 25;251(6):1799–1807. [PubMed] [Google Scholar]

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