Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Jan;141(1):382–385. doi: 10.1128/jb.141.1.382-385.1980

Role of transcriptional regulation and enzyme inactivation in the synthesis of Escherichia coli carbamoylphosphate synthase.

A Piérard, W Lissens, P Halleux, R Cunin, N Glansdorff
PMCID: PMC293605  PMID: 6153385

Abstract

The question of posttranscriptional control during cumulative repression of Escherichia coli carbamoylphosphate synthase has been examined by following the kinetics of repression and by comparing messenger ribonucleic acid and enzyme levels after growth under various conditions. The data provide no evidence for control of synthesis at a level other than transcription. They suggest, however, that enzyme inactivation (or turnover) plays a significant role in the establishment of repressive conditions.

Full text

PDF
384

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertrand K., Korn L., Lee F., Platt T., Squires C. L., Squires C., Yanofsky C. New features of the regulation of the tryptophan operon. Science. 1975 Jul 4;189(4196):22–26. doi: 10.1126/science.1094538. [DOI] [PubMed] [Google Scholar]
  2. Boy E., Theze J., Patte J. C. Transient regulation of enzyme synthesis in Escherichia coli. Mol Gen Genet. 1973;121(1):77–81. doi: 10.1007/BF00353695. [DOI] [PubMed] [Google Scholar]
  3. Cunin R., Boyen A., Pouwels P., Glansdorff N., Crabeel M. Parameters of gene expression in the bipolar argECBH operon of E. coli K12. The question of translational control. Mol Gen Genet. 1975 Sep 15;140(1):51–60. doi: 10.1007/BF00268988. [DOI] [PubMed] [Google Scholar]
  4. GLANSDORFF N. TOPOGRAPHY OF COTRANSDUCIBLE ARGININE MUTATIONS IN ESCHERICHIA COLI K-12. Genetics. 1965 Feb;51:167–179. doi: 10.1093/genetics/51.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glansdorff N., Dambly C., Palchaudhuri S., Crabeel M., Piérard A., Halleux P. Isolation and heteroduplex mapping of a lambda transducing bacteriophage carrying the structural genes for carbamoylphosphate synthase: regulation of enzyme synthesis in Escherichia coli K-12 lysogens. J Bacteriol. 1976 Jul;127(1):302–308. doi: 10.1128/jb.127.1.302-308.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kryzek R. A., Rogers P. Dual regulation by arginine of the expression of the Escherichia coli argECBH operon. J Bacteriol. 1976 Apr;126(1):348–364. doi: 10.1128/jb.126.1.348-364.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Lavallé R., De Hauwer G. Tryptophan messenger translation in Escherichia coli. J Mol Biol. 1970 Jul 28;51(2):435–447. doi: 10.1016/0022-2836(70)90153-1. [DOI] [PubMed] [Google Scholar]
  9. Lavallé R. Regulation at the level of translation in the arginine pathway of Escherichia coli K12. J Mol Biol. 1970 Jul 28;51(2):449–451. doi: 10.1016/0022-2836(70)90154-3. [DOI] [PubMed] [Google Scholar]
  10. Lee F., Yanofsky C. Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4365–4369. doi: 10.1073/pnas.74.10.4365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lissens W., Cunin R., Kelker N., Glansdorff N., Piérard A. In vitro synthesis of Escherichia coli carbamoylphosphate synthase: evidence for participation of the arginine repressor in cumulative repression. J Bacteriol. 1980 Jan;141(1):58–66. doi: 10.1128/jb.141.1.58-66.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mergeay M., Gigot D., Beckmann J., Glansdorff N., Piérard A. Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12. Mol Gen Genet. 1974;133(4):299–316. doi: 10.1007/BF00332706. [DOI] [PubMed] [Google Scholar]
  13. Piérard A., Glansdorff N., Gigot D., Crabeel M., Halleux P., Thiry L. Repression of Escherichia coli carbamoylphosphate synthase: relationships with enzyme synthesis in the arginine and pyrimidine pathways. J Bacteriol. 1976 Jul;127(1):291–301. doi: 10.1128/jb.127.1.291-301.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Piérard A., Glansdorff N., Mergeay M., Wiame J. M. Control of the biosynthesis of carbamoyl phosphate in Escherichia coli. J Mol Biol. 1965 Nov;14(1):23–36. doi: 10.1016/s0022-2836(65)80226-1. [DOI] [PubMed] [Google Scholar]
  15. Piérard A., Glansdorff N., Yashphe J. Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coli. Effects on carbamoyl phosphate and pyrimidine biosynthesis and on uracil uptake. Mol Gen Genet. 1972;118(3):235–245. doi: 10.1007/BF00333460. [DOI] [PubMed] [Google Scholar]
  16. Piérard A., Wiame J. M. Regulation and mutation affecting a glutamine dependent formation of carbamyl phosphate in Escherichia coli. Biochem Biophys Res Commun. 1964 Feb 18;15(1):76–81. doi: 10.1016/0006-291x(64)90106-8. [DOI] [PubMed] [Google Scholar]
  17. Pouwels P. H., Cunin R., Glansdorff N. Letter: Divergent transcription in the argECBH cluster of genes in Escherichia coli K12. J Mol Biol. 1974 Mar;83(3):421–424. doi: 10.1016/0022-2836(74)90288-5. [DOI] [PubMed] [Google Scholar]
  18. Trotta P. P., Pinkus L. M., Haschemeyer R. H., Meister A. Reversible dissociation of the monomer of glutamine-dependent carbamyl phosphate synthetase into catalytically active heavy and light subunits. J Biol Chem. 1974 Jan 25;249(2):492–499. [PubMed] [Google Scholar]
  19. Umbarger H. E. Amino acid biosynthesis and its regulation. Annu Rev Biochem. 1978;47:532–606. doi: 10.1146/annurev.bi.47.070178.002533. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES