Abstract
The effect of a number of conditions on the amount of cyanophycin granule polypeptide [multi-L-arginyl poly(L-aspartic acid)] formed in the unicellular cyanobacterium Aphanocapsa 6308 was determined. Light, CO2, sulfur, and phosphorus starvation as well as the addition of arginine to culture media increased the amount of cyanophycin granule polypeptide in cells when compared with that in cells grown under conditions optimal for growth. Nitrogen limitation and reduction of growth temperature to 30 degrees C decreased the amount of cyanophycin granule polypeptide on a dry-weight basis. Shift-up and shift-down experiments suggest cyanophycin granule polypeptide may be a reserve nitrogen polymer in Aphanocapsa 6308.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allen M. M., Weathers P. J. Structure and composition of cyanophycin granules in the cyanobacterium Aphanocapsa 6308. J Bacteriol. 1980 Feb;141(2):959–962. doi: 10.1128/jb.141.2.959-962.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lang N. J. The fine structure of blue-green algae. Annu Rev Microbiol. 1968;22:15–46. doi: 10.1146/annurev.mi.22.100168.000311. [DOI] [PubMed] [Google Scholar]
- Simon R. D. Cyanophycin Granules from the Blue-Green Alga Anabaena cylindrica: A Reserve Material Consisting of Copolymers of Aspartic Acid and Arginine. Proc Natl Acad Sci U S A. 1971 Feb;68(2):265–267. doi: 10.1073/pnas.68.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simon R. D. Measurement of the cyanophycin granule polypeptide contained in the blue-green alga Anabaena cylindrica. J Bacteriol. 1973 Jun;114(3):1213–1216. doi: 10.1128/jb.114.3.1213-1216.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simon R. D. The biosynthesis of multi-L-arginyl-poly(L-aspartic acid) in the filamentous cyanobacterium Anabaena cylindrica. Biochim Biophys Acta. 1976 Feb 13;422(2):407–418. doi: 10.1016/0005-2744(76)90151-0. [DOI] [PubMed] [Google Scholar]
- Simon R. D. The effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue green alga Anabaena cylindrica. Arch Mikrobiol. 1973;92(2):115–122. doi: 10.1007/BF00425009. [DOI] [PubMed] [Google Scholar]
- Simon R. D., Weathers P. Determination of the structure of the novel polypeptide containing aspartic acid and arginine which is found in Cyanobacteria. Biochim Biophys Acta. 1976 Jan 20;420(1):165–176. doi: 10.1016/0005-2795(76)90355-x. [DOI] [PubMed] [Google Scholar]
- Weathers P. J., Allen M. M. Variations in short term products of inorganic carbon fixation in exponential and stationary phase cultures of Aphanocapsa 6308. Arch Microbiol. 1978 Mar;116(3):231–234. doi: 10.1007/BF00417844. [DOI] [PubMed] [Google Scholar]