Abstract
An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be explained solely by the lack of exonuclease III activity, because other xth mutants tested, including a deletion mutant, repaired radiation-induced strand breaks at close to the normal rate.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Billen D., Hellermann G. R. Role of deoxyribonucleic acid polymerases and deoxyribonucleic acid ligase in x-ray-induced repair synthesis in toluene-treated Escherichia coli K-12. J Bacteriol. 1976 May;126(2):785–793. doi: 10.1128/jb.126.2.785-793.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Billen D., Hellermann G. R., Stallions D. R. Role for deoxyribonucleic acid ligase in deoxyribonucleic acid polymerase i-dependent repair synthesis in toluene-treated escherichia coli. J Bacteriol. 1975 Oct;124(1):585–588. doi: 10.1128/jb.124.1.585-588.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dean C., Pauling C. Properties of a deoxyribonucleic acid ligase mutant of Escherichia coli: x-ray sensitivity. J Bacteriol. 1970 May;102(2):588–589. doi: 10.1128/jb.102.2.588-589.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johansen I., Gurvin I., Rupp W. D. The formation of single-strand breaks in intracellular DNA by x-rays. Radiat Res. 1971 Dec;48(3):599–612. [PubMed] [Google Scholar]
- Kato T., Kondo S. Genetic and molecular characteristics of X-ray-sensitive mutants of Escherichia coli defective in repair synthesis. J Bacteriol. 1970 Nov;104(2):871–881. doi: 10.1128/jb.104.2.871-881.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laipis P. J., Ganesan A. T. In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3211–3214. doi: 10.1073/pnas.69.11.3211. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ljungquist S., Lindahl T., Howard-Flanders P. Methyl methane sulfonate-sensitive mutant of Escherichia coli deficient in an endonuclease specific for apurinic sites in deoxyribonucleic acid. J Bacteriol. 1976 May;126(2):646–653. doi: 10.1128/jb.126.2.646-653.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitzel-Landbeck L., Schutz G., Hagen U. In vitro repair of radiation-induced strand breaks in DNA. Biochim Biophys Acta. 1976 May 3;432(2):145–153. doi: 10.1016/0005-2787(76)90156-8. [DOI] [PubMed] [Google Scholar]
- Moses R. E., Richardson C. C. Replication and repair of DNA in cells of Escherichia coli treated with toluene. Proc Natl Acad Sci U S A. 1970 Oct;67(2):674–681. doi: 10.1073/pnas.67.2.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seeberg E., Strike P. Excision repair of ultraviolet-irradiated deoxyribonucleic acid in plasmolyzed cells of Escherichia coli. J Bacteriol. 1976 Mar;125(3):787–795. doi: 10.1128/jb.125.3.787-795.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Town C. D., Smith K. C., Kaplan H. S. DNA polymerase required for rapid repair of x-ray--induced DNA strand breaks in vivo. Science. 1971 May 21;172(3985):851–854. doi: 10.1126/science.172.3985.851. [DOI] [PubMed] [Google Scholar]
- Town C. D., Smith K. C., Kaplan H. S. The rapair of DNA single-strand breaks in E. coli K-12 x-irradiated in the presence or absence of oxygen; the influence of repair on cell survival. Radiat Res. 1973 Aug;55(2):334–345. [PubMed] [Google Scholar]
- White B. J., Hochhauser S. J., Cintron N. M., Weiss B. Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12. J Bacteriol. 1976 Jun;126(3):1082–1088. doi: 10.1128/jb.126.3.1082-1088.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yajko D. M., Weiss B. Mutations simultaneously affecting endonuclease II and exonuclease III in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Feb;72(2):688–692. doi: 10.1073/pnas.72.2.688. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Youngs D. A., Smith K. C. X-ray sensitivity and repair capacity of a polA1 exrA strain of Escherichia coli K-12. J Bacteriol. 1973 Apr;114(1):121–127. doi: 10.1128/jb.114.1.121-127.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]