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. 1980 Apr;142(1):221–228. doi: 10.1128/jb.142.1.221-228.1980

Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli.

J Felton, S Michaelis, A Wright
PMCID: PMC293934  PMID: 6102983

Abstract

Escherichia coli K-12 has two genes, asnA+ and asnB+, either one of which is able to satisfy the need of cells for asparagine. In order for a strain to have an auxotrophic requirement for asparagine, both genes must be mutationally inactivated. We obtained mutants with Tn5 inserted in asnB. asnB was mapped by conjugation and by three-factor P1 transductions at 15 min on the E coli K-12 linkage map, between ubiF and nagB. Specialized transducing phage lamba 781 supE was shown to carry asnB, as well as supE, ubiF, nagA, and nagB. asnA is the previously mapped ilv-linked asn locus, whiich is between uncB and rbs. E. coli C also has two asn genes, corresponding to asnA and asnB.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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