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. 1980 Apr;142(1):315–318. doi: 10.1128/jb.142.1.315-318.1980

New cryptic plasmid of Bacillus subtilis and restriction analysis of other plasmids found by general screening.

T Uozumi, A Ozaki, T Beppu, K Arima
PMCID: PMC293956  PMID: 6246066

Abstract

A new cryptic plasmid, pTA1030 (4.5 megadaltons, copy number 16), was characterized by restriction analysis, together with some other plasmids of Bacillus subtilis.

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Selected References

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  1. Bazaral M., Helinski D. R. Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J Mol Biol. 1968 Sep 14;36(2):185–194. doi: 10.1016/0022-2836(68)90374-4. [DOI] [PubMed] [Google Scholar]
  2. Bernhard K., Schrempf H., Goebel W. Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis. J Bacteriol. 1978 Feb;133(2):897–903. doi: 10.1128/jb.133.2.897-903.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang A. C., Cohen S. N. Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1030–1034. doi: 10.1073/pnas.71.4.1030. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen S. N., Chang A. C., Boyer H. W., Helling R. B. Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240–3244. doi: 10.1073/pnas.70.11.3240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ehrlich S. D., Bursztyn-Pettegrew H., Stroynowski I., Lederberg J. Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4145–4149. doi: 10.1073/pnas.73.11.4145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ehrlich S. D. DNA cloning in Bacillus subtilis. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1433–1436. doi: 10.1073/pnas.75.3.1433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gryczan T. J., Contente S., Dubnau D. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bacteriol. 1978 Apr;134(1):318–329. doi: 10.1128/jb.134.1.318-329.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gryczan T. J., Dubnau D. Construction and properties of chimeric plasmids in Bacillus subtilis. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1428–1432. doi: 10.1073/pnas.75.3.1428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Henneberry R. C., Carlton B. C. Characterization of the polydisperse closed circular deoxyribonucleic acid molecules of Bacillus megaterium. J Bacteriol. 1973 May;114(2):625–631. doi: 10.1128/jb.114.2.625-631.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Horinouchi S., Uozumi T., Hoshino T., Ozaki A., Nakajima S., Beppu T., Arima K. Molecular cloning and in vitro transcription of Bacillus subtilis plasmid in Escherichia coli. Mol Gen Genet. 1977 Nov 29;157(2):175–182. doi: 10.1007/BF00267395. [DOI] [PubMed] [Google Scholar]
  13. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  14. Keggins K. M., Lovett P. S., Duvall E. J. Molecular cloning of genetically active fragments of Bacillus DNA in Bacillus subtilis and properties of the vector plasmid pUB110. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1423–1427. doi: 10.1073/pnas.75.3.1423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lang D., Mitani M. Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970;9(3):373–379. doi: 10.1002/bip.1970.360090310. [DOI] [PubMed] [Google Scholar]
  16. Lovett P. S., Bramucci M. G. Plasmid deoxyribonucleic acid in Bacillus subtilis and Bacillus pumilus. J Bacteriol. 1975 Oct;124(1):484–490. doi: 10.1128/jb.124.1.484-490.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lovett P. S., Burdick B. D. Cryptic plasmid in Bacillus pumilus ATCC 7065. Biochem Biophys Res Commun. 1973 Sep 5;54(1):365–370. doi: 10.1016/0006-291x(73)90931-5. [DOI] [PubMed] [Google Scholar]
  18. Lovett P. S., Duvall E. J., Bramucci M. G., Taylor R. Host function specified by Bacillus pumilus plasmid pPL7065. Antimicrob Agents Chemother. 1977 Sep;12(3):435–437. doi: 10.1128/aac.12.3.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lovett P. S., Duvall E. J., Keggins K. M. Bacillus pumilus plasmid pPL10: properties and insertion into Bacillus subtilis 168 by transformation. J Bacteriol. 1976 Aug;127(2):817–828. doi: 10.1128/jb.127.2.817-828.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lovett P. S. Plasmid in Bacillus pumilus and the enhanced sporulation of plasmid-negative variants. J Bacteriol. 1973 Jul;115(1):291–298. doi: 10.1128/jb.115.1.291-298.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mahler I., Halvorson H. O. Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule. J Bacteriol. 1977 Jul;131(1):374–377. doi: 10.1128/jb.131.1.374-377.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  23. Shibata T., Ando T. The restriction endonucleases in Bacillus amyloliquefaciens N strain. Substrate specificities. Biochim Biophys Acta. 1976 Aug 18;442(2):184–196. doi: 10.1016/0005-2787(76)90489-5. [DOI] [PubMed] [Google Scholar]
  24. Tanaka T., Koshikawa T. Isolation and characterization of four types of plasmids from Bacillus subtilis (natto). J Bacteriol. 1977 Aug;131(2):699–701. doi: 10.1128/jb.131.2.699-701.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tanaka T., Kuroda M., Sakaguchi K. Isolation and characterization of four plasmids from Bacillus subtilis. J Bacteriol. 1977 Mar;129(3):1487–1494. doi: 10.1128/jb.129.3.1487-1494.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tanaka T., Sakaguchi K. Construction of a recombinant plasmid composed of B. subtilis leucine genes and a B. subtilis (natto) plasmid: its use as cloning vehicle in B. subtilis 168. Mol Gen Genet. 1978 Oct 24;165(3):269–276. doi: 10.1007/BF00332526. [DOI] [PubMed] [Google Scholar]
  27. Tanaka T., Weisblum B. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975 Jan;121(1):354–362. doi: 10.1128/jb.121.1.354-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wellauer P. K., Reeder R. H., Carroll D., Brown D. D., Deutch A., Higashinakagawa T., Dawid I. B. Amplified ribosomal DNA from Xenopus laevis has heterogeneous spacer lengths. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2823–2827. doi: 10.1073/pnas.71.7.2823. [DOI] [PMC free article] [PubMed] [Google Scholar]

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