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. 1980 May;142(2):701–713. doi: 10.1128/jb.142.2.701-713.1980

Transposon-mediated conjugational transmission of nonconjugative plasmids.

N J Crisona, J A Nowak, H Nagaishi, A J Clark
PMCID: PMC294055  PMID: 6247329

Abstract

When coresident with conjugative plasmid pNC21, the nonconjugative deletion F-prime pJC59, which retains the F transfer origin oriT, was transmitted to transconjugants at a frequency comparable to that of pNC21. In addition, pJC59 was transmitted as an independent plasmid, physically separate from pNC21, an example of plasmid donation. In contrast, two plasmids that are derived from F and deleted for the oriT site, pJC61 and pML31, were transmitted at frequencies 10(4) lower than that of pNC21. This low-frequency transmission was associated with the appearance of a new plasmid in the transconjugants. In the case of pML31, we determined that this new plasmid was a recombinant composed of pNC21 and pML31, the latter flanked by two copies of transposable element Tn3. We believe that this recombinant plasmid was formed as an intermediate in the transposition of Tn3 from pNC21 to pML31 and was the vehicle for conjugational transmission of pML31 genes by a process known as plasmid conduction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Skurray R. A., Thompson R., Helmuth R., Hall S., Beutin L., Clark A. J. Assignment of tra cistrons to EcoRI fragments of F sex factor DNA. J Bacteriol. 1978 Mar;133(3):1383–1392. doi: 10.1128/jb.133.3.1383-1392.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BECKWITH J. R., PARDEE A. B., AUSTRIAN R., JACOB F. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J Mol Biol. 1962 Dec;5:618–634. doi: 10.1016/s0022-2836(62)80090-4. [DOI] [PubMed] [Google Scholar]
  3. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blohm D., Goebel W. Restriction map of the antibiotic resistance plasmid R1drd-19 and its derivatives pKN102 (R1drd-19B2) and R1drd-16 for the enzymes BamHI, HindIII, EcoRI and SalI. Mol Gen Genet. 1978 Nov 29;167(2):119–127. doi: 10.1007/BF00266905. [DOI] [PubMed] [Google Scholar]
  6. CLARK A. J., ADELBERG E. A. Bacterial conjugation. Annu Rev Microbiol. 1962;16:289–319. doi: 10.1146/annurev.mi.16.100162.001445. [DOI] [PubMed] [Google Scholar]
  7. Cabello F., Timmis K., Cohen S. N. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature. 1976 Jan 29;259(5541):285–290. doi: 10.1038/259285a0. [DOI] [PubMed] [Google Scholar]
  8. Clark A. J., Warren G. J. Conjugal transmission of plasmids. Annu Rev Genet. 1979;13:99–125. doi: 10.1146/annurev.ge.13.120179.000531. [DOI] [PubMed] [Google Scholar]
  9. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  10. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Crisona N. J., Clark A. J. Increase in conjugational transmission frequency of nonconjugative plasmids. Science. 1977 Apr 8;196(4286):186–187. doi: 10.1126/science.322280. [DOI] [PubMed] [Google Scholar]
  12. Eggertsson G., Adelberg E. A. Map positions and specificities of suppressor mutations in Escherichia coli K-12. Genetics. 1965 Aug;52(2):319–340. doi: 10.1093/genetics/52.2.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Falkinham J. O., 3rd, Clark A. J. Genetic analysis of a double male strain of Escherichia coli K12. Genetics. 1974 Oct;78(2):633–644. doi: 10.1093/genetics/78.2.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gill R., Heffron F., Dougan G., Falkow S. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3. J Bacteriol. 1978 Nov;136(2):742–756. doi: 10.1128/jb.136.2.742-756.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Goebel W., Lindenmaier W., Pfeifer F., Schrempf H., Schelle B. Transposition and insertion of intact, deleted and enlarged ampicillin transposon Tn3 from mini-R1 (Rsc) plasmids into transfer factors. Mol Gen Genet. 1977 Nov 29;157(2):119–129. doi: 10.1007/BF00267389. [DOI] [PubMed] [Google Scholar]
  16. Graham R. S., Young F. E., Wilson G. A. Effect of site-specific endonuclease digestion on the thyP3 gene of bacteriophage phi 3T and the thyP11 gene of bacteriophage rho11. Gene. 1977 Mar;1(2):169–180. doi: 10.1016/0378-1119(77)90027-0. [DOI] [PubMed] [Google Scholar]
  17. Grindley N. D., Sherratt D. J. Sequence analysis at IS1 insertion sites: models for transposition. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1257–1261. doi: 10.1101/sqb.1979.043.01.142. [DOI] [PubMed] [Google Scholar]
  18. Guyer M. S., Clark A. J. cis-Dominant, transfer-deficient mutants of the Escherichia coli K-12 F sex factor. J Bacteriol. 1976 Jan;125(1):233–247. doi: 10.1128/jb.125.1.233-247.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Guyer M. S., Davidson N., Clark A. J. Heteroduplex analysis of tra delta f' plasmids and the mechanism of their formation. J Bacteriol. 1977 Sep;131(3):970–980. doi: 10.1128/jb.131.3.970-980.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Guyer M. S. The gamma delta sequence of F is an insertion sequence. J Mol Biol. 1978 Dec 15;126(3):347–365. doi: 10.1016/0022-2836(78)90045-1. [DOI] [PubMed] [Google Scholar]
  21. Heffron F., Bedinger P., Champoux J. J., Falkow S. Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci U S A. 1977 Feb;74(2):702–706. doi: 10.1073/pnas.74.2.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Hershfield V., Boyer H. W., Chow L., Helinski D. R. Characterization of a mini-ColC1 plasmid. J Bacteriol. 1976 Apr;126(1):447–453. doi: 10.1128/jb.126.1.447-453.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Hershfield V., Boyer H. W., Yanofsky C., Lovett M. A., Helinski D. R. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455–3459. doi: 10.1073/pnas.71.9.3455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Kopecko D. J., Brevet J., Cohen S. N. Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids. J Mol Biol. 1976 Dec;108(2):333–360. doi: 10.1016/s0022-2836(76)80124-6. [DOI] [PubMed] [Google Scholar]
  25. Kopecko D. J., Cohen S. N. Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1373–1377. doi: 10.1073/pnas.72.4.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Lovett M. A., Helinski D. R. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid. J Bacteriol. 1976 Aug;127(2):982–987. doi: 10.1128/jb.127.2.982-987.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. MacHattie L. A., Shapiro J. A. Chromosomal integration of phage lambda by means of a DNA insertion element. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1490–1494. doi: 10.1073/pnas.75.3.1490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Manis J. J., Kline B. C. Restriction endonuclease mapping and mutagenesis of the F sex factor replication region. Mol Gen Genet. 1977 Apr 29;152(3):175–182. doi: 10.1007/BF00268815. [DOI] [PubMed] [Google Scholar]
  29. Meyer R., Boch G., Shapiro J. Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element. Mol Gen Genet. 1979 Mar 9;171(1):7–13. doi: 10.1007/BF00274009. [DOI] [PubMed] [Google Scholar]
  30. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Novick R. P., Clowes R. C., Cohen S. N., Curtiss R., 3rd, Datta N., Falkow S. Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev. 1976 Mar;40(1):168–189. doi: 10.1128/br.40.1.168-189.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Shapiro J. A. Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1933–1937. doi: 10.1073/pnas.76.4.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Skurray R. A., Guyer M. S., Timmis K., Cabello F., Cohen S. N., Davidson N., Clark A. J. Replication region fragments cloned from Flac+ are identical to EcoRI fragment f5 of F. J Bacteriol. 1976 Sep;127(3):1571–1575. doi: 10.1128/jb.127.3.1571-1575.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Skurray R. A., Willetts N., Reeves P. Effect of tra mutations on F factor-specified immunity to lethal zygosis. Mol Gen Genet. 1976 Jul 23;146(2):161–165. doi: 10.1007/BF00268084. [DOI] [PubMed] [Google Scholar]
  35. Templin A., Kushner S. R., Clark A. J. Genetic analysis of mutations indirectly suppressing recB and recC mutations. Genetics. 1972 Oct;72(2):105–115. [PMC free article] [PubMed] [Google Scholar]
  36. Templin A., Margossian L., Clark A. J. Suppressibility of recA, recB, and recC mutations by nonsense suppressors. J Bacteriol. 1978 May;134(2):590–596. doi: 10.1128/jb.134.2.590-596.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Toussaint A., Faelen M. Connecting two unrelated DNA sequences with a Mu dimer. Nat New Biol. 1973 Mar 7;242(114):1–4. doi: 10.1038/newbio242001a0. [DOI] [PubMed] [Google Scholar]
  38. WATANABE T. Infective heredity of multiple drug resistance in bacteria. Bacteriol Rev. 1963 Mar;27:87–115. doi: 10.1128/br.27.1.87-115.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Warren G., Sherratt D. Complementation of transfer deficient ColE1 mutants. Mol Gen Genet. 1977 Mar 7;151(2):197–201. doi: 10.1007/BF00338695. [DOI] [PubMed] [Google Scholar]
  40. Willetts N. S., Clark A. J., Low B. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J Bacteriol. 1969 Jan;97(1):244–249. doi: 10.1128/jb.97.1.244-249.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Willetts N. S. Location of the origin of transfer of the sex factor F. J Bacteriol. 1972 Nov;112(2):773–778. doi: 10.1128/jb.112.2.773-778.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Williams R. C. Use of polylysine for adsorption of nuclei acids and enzymes to electron microscope specimen films. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2311–2315. doi: 10.1073/pnas.74.6.2311. [DOI] [PMC free article] [PubMed] [Google Scholar]

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