Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Jun;142(3):925–930. doi: 10.1128/jb.142.3.925-930.1980

Chromosomally integrated conjugative plasmids are common in antibiotic-resistant Haemophilus influenzae.

J H Stuy
PMCID: PMC294118  PMID: 6966629

Abstract

Twenty-three highly antibiotic-resistant strains of Haemophilus influenzae and two of Haemophilus parainfluenzae without detectable large plasmids were examined for conjugative transfer of their resistance to H. influenzae strain Rd or to other strains. Very inefficient transfer was observed for 18 H. influenzae strains and 1 H. parainfluenzae strain. All H. influenzae transcipients carried a large plasmid, and they were in turn efficient donors of their resistances in standard conjugation crosses with isogenic recipients. This was not seen for the H. parainfluenzae transcipients. It is concluded that most of the original antibiotic-resistant cultures carried an integrated conjugative R plasmid which had been excised in a few cells in each population. It was these cells which transferred resistance in the primary crosses.

Full text

PDF
925

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER H. E., LEIDY G. Determination of inherited traits of H. influenzae by desoxyribonucleic acid fractions isolated from type-specific cells. J Exp Med. 1951 Apr 1;93(4):345–359. doi: 10.1084/jem.93.4.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnhart B. J., Cox S. H. Radiation-sensitive and radiation-resistant mutants of Haemophilus influenzae. J Bacteriol. 1968 Jul;96(1):280–282. doi: 10.1128/jb.96.1.280-282.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bendler J. W., 3rd Physical size of the donor locus and transmission of Haemophilus influenzae ampicillin resistance genes by deoxyribonucleic acid-mediated transformation. J Bacteriol. 1976 Jan;125(1):197–204. doi: 10.1128/jb.125.1.197-204.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chesney R. H., Scott J. R., Vapnek D. Integration of the plasmid prophages P1 and P7 into the chromosome of Escherichia coli. J Mol Biol. 1979 May 15;130(2):161–173. doi: 10.1016/0022-2836(79)90424-8. [DOI] [PubMed] [Google Scholar]
  5. Elwell L. P., De Graaff J., Seibert D., Falkow S. Plasmid-linked ampicillin resistance in haempohilus influenza type b. Infect Immun. 1975 Aug;12(2):404–410. doi: 10.1128/iai.12.2.404-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elwell L. P., Saunders J. R., Richmond M. H., Falkow S. Relationships among some R plasmids found in Haemophilus influenzae. J Bacteriol. 1977 Jul;131(1):356–362. doi: 10.1128/jb.131.1.356-362.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goffic F. L., Moreau N., Siegrist S., Goldstein F. W., Acar J. C. La resistance plasmidique de Haemophilus sp. aux antibiotiques aminoglycosidiques : isolement et etude d'une nouvelle phosphotransférase. Ann Microbiol (Paris) 1977 May-Jun;128A(4):383–391. [PubMed] [Google Scholar]
  8. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hansman D. Haemophilus influenzae type B resistant to tetracycline isolated from children with meningitis. Lancet. 1975 Nov 8;2(7941):893–896. doi: 10.1016/s0140-6736(75)92128-5. [DOI] [PubMed] [Google Scholar]
  10. Kooistra J., Setlow J. K. Similarity in properties and mapping of three Rec mutants of Haemophilus influenzae. J Bacteriol. 1976 Jul;127(1):327–333. doi: 10.1128/jb.127.1.327-333.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STUY J. H. Transformability of Haemophilus influenzae. J Gen Microbiol. 1962 Nov;29:537–549. doi: 10.1099/00221287-29-3-537. [DOI] [PubMed] [Google Scholar]
  13. Saunders J. R., Elwell L. P., Falkow S., Sykes R. B., Richmond M. H. beta-lactamases and R-plasmids of Haemophilus influenzae. Scand J Infect Dis Suppl. 1978;(13):16–22. [PubMed] [Google Scholar]
  14. Setlow J. K., Brown D. C., Boling M. E., Mattingly A., Gordon M. P. Repair of deoxyribonucleic acid in Haemophilus influenzae. I. X-ray sensitivity of ultraviolet-sensitive mutants and their behavior as hosts to ultraviolet-irradiated bacteriophage and transforming deoxyribonucleic acid. J Bacteriol. 1968 Feb;95(2):546–558. doi: 10.1128/jb.95.2.546-558.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stuy J. H. Plasmid transfer in Haemophilus influenzae. J Bacteriol. 1979 Aug;139(2):520–529. doi: 10.1128/jb.139.2.520-529.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Williams J. D., Andrews J. Sensitivity of Haemophilus influenzae to antibiotics. Br Med J. 1974 Jan 26;1(5899):134–137. doi: 10.1136/bmj.1.5899.134. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES