Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Jun;142(3):1045–1048. doi: 10.1128/jb.142.3.1045-1048.1980

In vitro translation of messenger ribonucleic acid from sporulating and nonsporulating strains of Bacillus subtilis.

M Arnaud, I Mahler, H O Halvorson, H Boschwitz, A Keynan
PMCID: PMC294138  PMID: 6155373

Abstract

An Escherichia coli translation system supplemented with ribonucleic acid from sporulating Bacillus subtilis produces unique polypeptides which are missing among translation products of ribonucleic acid from six early sporulation mutants.

Full text

PDF
1048

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. François M., Glatron P., Rapoport G. Translation of a stable mRNA fraction from sporulating cells of Bacillus thuringiensis in a cell-free system from Escherichia coli. Structural homologies between the native crystal protein and the products synthesized in vitro. Biochimie. 1976;58(1-2):119–129. doi: 10.1016/s0300-9084(76)80362-8. [DOI] [PubMed] [Google Scholar]
  2. Hopper J. E., Ko G., Young E. T. Comparative analysis of the in vivo and in vitro expression of bacteriophage T7 messenger RNAs during infection of Escherichia coli. J Mol Biol. 1975 Jun 5;94(4):539–554. doi: 10.1016/0022-2836(75)90320-4. [DOI] [PubMed] [Google Scholar]
  3. Hutchison K. W., Halvorson H. O. Cloning of randomly sheared DNA fragments from a phi 105 lysogen of Bacillus subtilis identification of prophage-containing clones. Gene. 1980 Feb;8(3):267–278. doi: 10.1016/0378-1119(80)90004-9. [DOI] [PubMed] [Google Scholar]
  4. Legault-Demare L., Chambliss G. H. Natural messenger ribonucleic acid-directed cell-free protein-synthesizing system of Bacillus subtilis. J Bacteriol. 1974 Dec;120(3):1300–1307. doi: 10.1128/jb.120.3.1300-1307.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Linn T., Losick R. The program of protein synthesis during sporulation in Bacillus subtilis. Cell. 1976 May;8(1):103–114. doi: 10.1016/0092-8674(76)90191-4. [DOI] [PubMed] [Google Scholar]
  6. Mellado R. P., Vinuela E., Salas M. Isolation of a strong suppressor of nonsense mutations in Bacillus subtilis. Eur J Biochem. 1976 May 17;65(1):213–223. doi: 10.1111/j.1432-1033.1976.tb10408.x. [DOI] [PubMed] [Google Scholar]
  7. Nakayama T., Munoz L. E., Sadaie Y., Doi R. H. Spore coat protein synthesis in cell-free systems from sporulating cells of Bacillus subtilis. J Bacteriol. 1978 Sep;135(3):952–960. doi: 10.1128/jb.135.3.952-960.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Paterson B. M., Roberts B. E., Kuff E. L. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4370–4374. doi: 10.1073/pnas.74.10.4370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rapoport G., Klier A., Billault A., Fargette F., Dedonder R. Construction of a colony bank of E. coli containing hybrid plasmids representative of the Bacillus subtilis 168 genome. Expression of functions harbored by the recombinant plasmids in B. subtilis. Mol Gen Genet. 1979 Oct 3;176(2):239–245. doi: 10.1007/BF00273218. [DOI] [PubMed] [Google Scholar]
  10. Schaeffer P., Millet J., Aubert J. P. Catabolic repression of bacterial sporulation. Proc Natl Acad Sci U S A. 1965 Sep;54(3):704–711. doi: 10.1073/pnas.54.3.704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Segall J., Losick R. Cloned Bacillus subtilis DNA containing a gene that is activated early during sporulation. Cell. 1977 Aug;11(4):751–761. doi: 10.1016/0092-8674(77)90289-6. [DOI] [PubMed] [Google Scholar]
  12. Sterlini J. M., Mandelstam J. Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969 Jun;113(1):29–37. doi: 10.1042/bj1130029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  14. Woolford J. L., Jr, Rosbash M. The use of R-looping for structural gene identification and mRNA purification. Nucleic Acids Res. 1979 Jun 11;6(7):2483–2497. doi: 10.1093/nar/6.7.2483. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES