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. 1980 Jul;143(1):135–141. doi: 10.1128/jb.143.1.135-141.1980

Outer Membrane-Dependent Transport Systems in Escherichia coli: Effect of Repression or Cessation of Colicin Receptor Synthesis on Colicin Receptor Activities

Robert J Kadner 1, Gail McElhaney 1
PMCID: PMC294196  PMID: 6447139

Abstract

Proteins in the outer membrane of gram-negative bacteria serve as general porins or as receptors for specific nutrient transport systems. Many of these proteins are also used as receptors initiating the processes of colicin or phage binding and uptake. The functional activities of several outer membrane proteins in Escherichia coli K-12 were followed after cessation or repression of their synthesis. Cessation of receptor synthesis was accomplished with a thermolabile suppressor activity acting on amber mutations in btuB (encoding the receptor for vitamin B12, the E colicins, and phage BF23) and in fepA (encoding the receptor for ferric enterochelin and colicins B and D). After cessation of receptor synthesis, cells rapidly became insensitive to the colicins using that receptor. Treatment with spectinomycin or rifampin blocked appearance of insensitive cells and even increased susceptibility to colicin E1. Insensitivity to phage BF23 appeared only after a lag of about one division time, and the receptors remained functional for B12 uptake throughout. Therefore, possession of receptor is insufficient for colicin sensitivity, and some interaction of receptor with subsequent uptake components is indicated. Another example of physiological alteration of colicin sensitivity is the protection against many of the tonB-dependent colicins afforded by provision of iron-supplying siderophores. The rate of acquisition of this nonspecific protection was found to be consistent with the repression of receptor synthesis, rather than through direct and immediate effects on the tonB product or other components of colicin uptake or action.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bassford P. J., Jr, Kadner R. J., Schnaitman C. A. Biosynthesis of the outer membrane receptor for vitamin B12, E colicins, and bacteriophage BF23 by Escherichia coli: kinetics of phenotypic expression after the introduction of bfe+ and bfe alleles. J Bacteriol. 1977 Jan;129(1):265–275. doi: 10.1128/jb.129.1.265-275.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bassford P. J., Jr, Schnaitman C. A., Kadner R. J. Functional stability of the bfe and tonB gene products in Escherichia coli. J Bacteriol. 1977 May;130(2):750–758. doi: 10.1128/jb.130.2.750-758.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bassford P. J., Jr, kadner R. J. Genetic analysis of components involved in vitamin B12 uptake in Escherichia coli. J Bacteriol. 1977 Dec;132(3):796–805. doi: 10.1128/jb.132.3.796-805.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Begg K. J., Doanachie W. D. Growth of the Escherichia coli cell surface. J Bacteriol. 1977 Mar;129(3):1524–1536. doi: 10.1128/jb.129.3.1524-1536.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies J. K., Reeves P. Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group B. J Bacteriol. 1975 Jul;123(1):96–101. doi: 10.1128/jb.123.1.96-101.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fiss E. H., Hollifield W. C., Jr, Neilands J. B. Absence of ferric enterobactin receptor modification activity in mutants of Escherichia coli K-12 lacking protein a. Biochem Biophys Res Commun. 1979 Nov 14;91(1):29–34. doi: 10.1016/0006-291x(79)90578-3. [DOI] [PubMed] [Google Scholar]
  8. Kadner R. J., Bassford P. J., Jr, Pugsley A. P. Colicin receptors and the mechanisms of colicin uptake. Zentralbl Bakteriol Orig A. 1979 Jun;244(1):90–104. [PubMed] [Google Scholar]
  9. Kadner R. J., Bassford P. J., Jr Relation of cell growth and colicin tolerance to vitamin B12 uptake in Escherichia coli. J Bacteriol. 1977 Jan;129(1):254–264. doi: 10.1128/jb.129.1.254-264.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kadner R. J., McElhaney G. Outer membrane-dependent transport systems in Escherichia coli: turnover of TonB function. J Bacteriol. 1978 Jun;134(3):1020–1029. doi: 10.1128/jb.134.3.1020-1029.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kadner R. J. Repression of synthesis of the vitamin B12 receptor in Escherichia coli. J Bacteriol. 1978 Dec;136(3):1050–1057. doi: 10.1128/jb.136.3.1050-1057.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Konisky J., Soucek S., Frick K., Davies J. K., Hammond C. Relationship between the transport of iron and the amount of specific colicin Ia membrane receptors in Escherichia coli. J Bacteriol. 1976 Jul;127(1):249–257. doi: 10.1128/jb.127.1.249-257.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lau C., Richards F. M. Behavior of colicins E1, E2, and E3 attached to sephadex beads. Biochemistry. 1976 Feb 10;15(3):666–671. doi: 10.1021/bi00648a034. [DOI] [PubMed] [Google Scholar]
  14. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pugsley A. P., Reeves P. Characterization of group B colicin-resistant mutants of Escherichia coli K-12: colicin resistance and the role of enterochelin. J Bacteriol. 1976 Jul;127(1):218–228. doi: 10.1128/jb.127.1.218-228.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pugsley A. P., Reeves P. Increased production of the outer membrane receptors for colicins B, D and M by Escherichia coli under iron starvation. Biochem Biophys Res Commun. 1976 Jun 7;70(3):846–853. doi: 10.1016/0006-291x(76)90669-0. [DOI] [PubMed] [Google Scholar]
  17. Pugsley A. P., Reeves P. The role of colicin receptors in the uptake of ferrienterochelin by Escherichia coli K-12. Biochem Biophys Res Commun. 1977 Feb 7;74(3):903–911. doi: 10.1016/0006-291x(77)91604-7. [DOI] [PubMed] [Google Scholar]
  18. Watson D. H., Sherratt D. J. In vivo proteolytic cleavage of colicins requires specific receptor binding. Nature. 1979 Mar 22;278(5702):362–364. doi: 10.1038/278362a0. [DOI] [PubMed] [Google Scholar]
  19. Wayne R., Frick K., Neilands J. B. Siderophore protection against colicins M, B, V, and Ia in Escherichia coli. J Bacteriol. 1976 Apr;126(1):7–12. doi: 10.1128/jb.126.1.7-12.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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