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. 1980 Aug;143(2):801–808. doi: 10.1128/jb.143.2.801-808.1980

Regulation of nitrogen utilization of hisT mutants of Salmonella typhimurium.

S A Rosenfeld, J E Brenchley
PMCID: PMC294366  PMID: 7009565

Abstract

Mutations in the hisT gene of Salmonella typhimurium alter pseudouridine synthetase I, the enzyme that modifies two uridines in the anticodon loop of numerous transfer ribonucleic acid species. We have examined two strains carrying different hisT mutations for their ability to grow on a variety of nitrogen sources. The hisT mutants grew more rapidly than did hisT+ strains with either arginine or proline as the nitrogen source and glucose as the carbon source. The hisT mutations were transduced into new strains to show that these growth properties were due to the hisT mutations. The hisT mutations did not influence the growth of mutants having altered glutamine synthetase regulation. Assays of the three primary ammonia-assimilatory enzymes, glutamate dehydrogenase, glutamine synthetase, and glutamate synthase, showed that glutamate synthase activities were lower in hisT mutants than in isogenic hisT+ controls; however, the glutamate dehydrogenase activity was about threefold higher in the hisT strains grown in glucose-arginine medium. The results suggest that the controls for enzyme synthesis for nitrogen utilization respond either directly or indirectly to transfer ribonucleic acid species affected by the hisT mutation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brenchley J. E. Effect of methionine sulfoximine and methionine sulfone on glutamate synthesis in Klebsiella aerogenes. J Bacteriol. 1973 May;114(2):666–673. doi: 10.1128/jb.114.2.666-673.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brenchley J. E., Ingraham J. L. Characterization of a cold-sensitive hisW mutant of Salmonella typhimurium. J Bacteriol. 1973 May;114(2):528–536. doi: 10.1128/jb.114.2.528-536.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brenchley J. E., Prival M. J., Magasanik B. Regulation of the synthesis of enzymes responsible for glutamate formation in Klebsiella aerogenes. J Biol Chem. 1973 Sep 10;248(17):6122–6128. [PubMed] [Google Scholar]
  4. Brenner M., Ames B. N. Histidine regulation in Salmonella typhimurium. IX. Histidine transfer ribonucleic acid of the regulatory mutants. J Biol Chem. 1972 Feb 25;247(4):1080–1088. [PubMed] [Google Scholar]
  5. Brown B. A., Lax S. R., Liang L., Dabney B. J., Spremulli L. L., Ravel J. M. Repression of the tyrosine, lysine, and methionine biosynthetic pathways in a hisT mutant of Salmonella typhimurium. J Bacteriol. 1977 Feb;129(2):1168–1170. doi: 10.1128/jb.129.2.1168-1170.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chang G. W., Roth J. R., Ames B. N. Histidine regulation in Salmonella typhimurium. 8. Mutations of the hisT gene. J Bacteriol. 1971 Oct;108(1):410–414. doi: 10.1128/jb.108.1.410-414.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cheung A., Morgan S., Low K. B., Söll D. Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. VI. Mutants with increased levels of glutaminyl-transfer ribonucleic acid synthetase and of glutamine transfer ribonucleic acid. J Bacteriol. 1979 Jul;139(1):176–184. doi: 10.1128/jb.139.1.176-184.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cortese R., Kammen H. O., Spengler S. J., Ames B. N. Biosynthesis of pseudouridine in transfer ribonucleic acid. J Biol Chem. 1974 Feb 25;249(4):1103–1108. [PubMed] [Google Scholar]
  9. Dendinger S. M., Patil L. G., Brenchley J. E. Salmonella typhimurium mutants with altered glutamate dehydrogenase and glutamate synthase activities. J Bacteriol. 1980 Jan;141(1):190–198. doi: 10.1128/jb.141.1.190-198.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Folk W. R., Yaniv M. Coding properties and nucleotide sequences of E. coli glutamine tRNAs. Nat New Biol. 1972 Jun 7;237(75):165–166. doi: 10.1038/newbio237165a0. [DOI] [PubMed] [Google Scholar]
  11. Funanage V. L., Ayling P. D., Dendinger S. M., Brenchley J. E. Salmonella typhimurium LT-2 mutants with altered glutamine synthetase levels and amino acid uptake activities. J Bacteriol. 1978 Nov;136(2):588–596. doi: 10.1128/jb.136.2.588-596.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gauss D. H., Grüter F., Sprinzl M. Compilation of tRNA sequences. Nucleic Acids Res. 1979 Jan;6(1):r1–r19. [PMC free article] [PubMed] [Google Scholar]
  13. Keller E. B., Calvo J. M. Alternative secondary structures of leader RNAs and the regulation of the trp, phe, his, thr, and leu operons. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6186–6190. doi: 10.1073/pnas.76.12.6186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kustu S. G., McFarland N. C., Hui S. P., Esmon B., Ames G. F. Nitrogen control of Salmonella typhimurium: co-regulation of synthesis of glutamine synthetase and amino acid transport systems. J Bacteriol. 1979 Apr;138(1):218–234. doi: 10.1128/jb.138.1.218-234.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kustu S., Burton D., Garcia E., McCarter L., McFarland N. Nitrogen control in Salmonella: regulation by the glnR and glnF gene products. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4576–4580. doi: 10.1073/pnas.76.9.4576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Lapointe J., Delcuve G., Duplain L. Derepressed levels of glutamate synthase and glutamine synthetase in Escherichia coli mutants altered in glutamyl-transfer ribonucleic acid synthetase. J Bacteriol. 1975 Sep;123(3):843–850. doi: 10.1128/jb.123.3.843-850.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morgan S., Körner A., Low K. B., Söll D. Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. I. Isolation and characterization of a mutant with elevated levels of tRNAGln 1. J Mol Biol. 1977 Dec 25;117(4):1013–1031. doi: 10.1016/s0022-2836(77)80010-7. [DOI] [PubMed] [Google Scholar]
  19. Murray M. L., Hartman P. E. Overproduction of hisH and hisF gene products leads to inhibition of cell cell division in Salmonella. Can J Microbiol. 1972 May;18(5):671–681. doi: 10.1139/m72-105. [DOI] [PubMed] [Google Scholar]
  20. Pahel G., Tyler B. A new glnA-linked regulatory gene for glutamine synthetase in Escherichia coli. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4544–4548. doi: 10.1073/pnas.76.9.4544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Quay S. C., Lawther R. P., Hatfield G. W., Oxender D. L. Branched-chain amino acid transport regulation in mutants blocked in tRNA maturation and transcriptional termination. J Bacteriol. 1978 May;134(2):683–686. doi: 10.1128/jb.134.2.683-686.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rosenfeld S. A., Brenchley J. Rapid methods for generalized transduction of Salmonella typhimurium mutants. J Bacteriol. 1979 Apr;138(1):261–263. doi: 10.1128/jb.138.1.261-263.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Roth J. R., Antón D. N., Hartman P. E. Histidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties. J Mol Biol. 1966 Dec 28;22(2):305–323. doi: 10.1016/0022-2836(66)90134-3. [DOI] [PubMed] [Google Scholar]
  24. Silbert D. F., Fink G. R., Ames B. N. Histidine regulatory mutants in Salmonella typhimurium 3. A class of regulatory mutants deficient in tRNA for histidine. J Mol Biol. 1966 Dec 28;22(2):335–347. doi: 10.1016/0022-2836(66)90136-7. [DOI] [PubMed] [Google Scholar]
  25. Singer C. E., Smith G. R., Cortese R., Ames B. N. [Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications]. Nat New Biol. 1972 Jul 19;238(81):72–74. doi: 10.1038/newbio238072a0. [DOI] [PubMed] [Google Scholar]
  26. Turnbough C. L., Jr, Neill R. J., Landsberg R., Ames B. N. Pseudouridylation of tRNAs and its role in regulation in Salmonella typhimurium. J Biol Chem. 1979 Jun 25;254(12):5111–5119. [PubMed] [Google Scholar]
  27. Tyler B. Regulation of the assimilation of nitrogen compounds. Annu Rev Biochem. 1978;47:1127–1162. doi: 10.1146/annurev.bi.47.070178.005403. [DOI] [PubMed] [Google Scholar]

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