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. 1980 Aug;143(2):1049–1053. doi: 10.1128/jb.143.2.1049-1053.1980

Functional analysis of minichromosome replication: bidirectional and unidirectional replication from the Escherichia coli replication origin, oriC.

M Meijer, W Messer
PMCID: PMC294416  PMID: 7009545

Abstract

Replicating molecules of minichromosomes pCM959 and pOC24 were analyzed by electron microscopy. Replication of pCM959 proceeded bidirectionally from the replication origin, oriC, in about 60% of the molecules; the rest of the molecules replicated unidirectionally in either direction. pOC24, in which deoxyribonucleic acid to the right (clockwise) of the oriC segment is deleted, seemed to replicate predominantly unidirectionally counterclockwise from oriC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bird R. E., Louarn J., Martuscelli J., Caro L. Origin and sequence of chromosome replication in Escherichia coli. J Mol Biol. 1972 Oct 14;70(3):549–566. doi: 10.1016/0022-2836(72)90559-1. [DOI] [PubMed] [Google Scholar]
  3. Blattner F. R., Dahlberg J. E. RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed? Nat New Biol. 1972 Jun 21;237(77):227–232. doi: 10.1038/newbio237227a0. [DOI] [PubMed] [Google Scholar]
  4. Cabello F., Timmis K., Cohen S. N. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature. 1976 Jan 29;259(5541):285–290. doi: 10.1038/259285a0. [DOI] [PubMed] [Google Scholar]
  5. Edlund T., Gustafsson P., Wolf-Watz H. Effect of thymine concentration on the mode of chromosomal replication in Escherichia coli K-12. J Mol Biol. 1976 Dec;108(2):295–303. doi: 10.1016/s0022-2836(76)80122-2. [DOI] [PubMed] [Google Scholar]
  6. Eichenlaub R., Figurski D., Helinski D. R. Bidirection replication from a unique origin in a mini-F plasmid. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138–1141. doi: 10.1073/pnas.74.3.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hiraga S. Novel F prime factors able to replicate in Escherichia coli Hfr strains. Proc Natl Acad Sci U S A. 1976 Jan;73(1):198–202. doi: 10.1073/pnas.73.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirota Y., Yasuda S., Yamada M., Nishimura A., Sugimoto K., Sugisaki H., Oka A., Takanami M. Structural and functional properties of the Escherichia coli origin of DNA replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):129–138. doi: 10.1101/sqb.1979.043.01.019. [DOI] [PubMed] [Google Scholar]
  9. Hobom G., Grosschedl R., Lusky M., Scherer G., Schwarz E., Kössel H. Functional analysis of the replicator structure of lambdoid bacteriophage DNAs. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):165–178. doi: 10.1101/sqb.1979.043.01.023. [DOI] [PubMed] [Google Scholar]
  10. Jacq A., Kohiyama M. A DNA-binding protein specific for the early replicated region of the chromosome obtained from Escherichia coli membrane fractions. Eur J Biochem. 1980 Mar;105(1):25–31. doi: 10.1111/j.1432-1033.1980.tb04470.x. [DOI] [PubMed] [Google Scholar]
  11. Masters M., Broda P. Evidence for the bidirectional replications of the Escherichia coli chromosome. Nat New Biol. 1971 Aug 4;232(31):137–140. doi: 10.1038/newbio232137a0. [DOI] [PubMed] [Google Scholar]
  12. Masters M. Strains of Escherichia coli diploid for the chromosomal origin of DNA replication. Mol Gen Genet. 1975 Dec 30;143(1):105–111. doi: 10.1007/BF00269427. [DOI] [PubMed] [Google Scholar]
  13. McMacken R., Ueda K., Kornberg A. Migration of Escherichia coli dnaB protein on the template DNA strand as a mechanism in initiating DNA replication. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4190–4194. doi: 10.1073/pnas.74.10.4190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Meijer M., Beck E., Hansen F. G., Bergmans H. E., Messer W., von Meyenburg K., Schaller H. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome. Proc Natl Acad Sci U S A. 1979 Feb;76(2):580–584. doi: 10.1073/pnas.76.2.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Messer W., Bergmans H. E., Meijer M., Womack J. E., Hansen F. G., von Meyenburg K. Mini-chromosomes: plasmids which carry the E. coli replication origin. Mol Gen Genet. 1978 Jul 4;162(3):269–275. doi: 10.1007/BF00268852. [DOI] [PubMed] [Google Scholar]
  16. Messer W., Meijer M., Bergmans H. E., Hansen F. G., von Meyenburg K., Beck E., Schaller H. Origin of replication, oriC, of the Escherichia coli K12 chromosome: nucleotide sequence. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):139–145. doi: 10.1101/sqb.1979.043.01.020. [DOI] [PubMed] [Google Scholar]
  17. Miki T., Hiraga S., Nagata T., Yura T. Bacteriophage lambda carrying the Escherichia coli chromosomal region of the replication origin. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5099–5103. doi: 10.1073/pnas.75.10.5099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moore D. D., Denniston-Thompson K., Kruger K. E., Furth M. E., Williams B. G., Daniels D. L., Blattner F. R. Dissection and comparative anatomy of the origins of replication of lambdoid phages. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):155–163. doi: 10.1101/sqb.1979.043.01.022. [DOI] [PubMed] [Google Scholar]
  19. Oka A., Sugimoto K., Takanami M., Hirota Y. Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication. Mol Gen Genet. 1980 Apr;178(1):9–20. doi: 10.1007/BF00267207. [DOI] [PubMed] [Google Scholar]
  20. Prescott D. M., Kuempel P. L. Bidirectional replication of the chromosome in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2842–2845. doi: 10.1073/pnas.69.10.2842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schnös M., Inman R. B. Position of branch points in replicating lambda DNA. J Mol Biol. 1970 Jul 14;51(1):61–73. doi: 10.1016/0022-2836(70)90270-6. [DOI] [PubMed] [Google Scholar]
  22. Sugimoto K., Oka A., Sugisaki H., Takanami M., Nishimura A., Yasuda Y., Hirota Y. Nucleotide sequence of Escherichia coli K-12 replication origin. Proc Natl Acad Sci U S A. 1979 Feb;76(2):575–579. doi: 10.1073/pnas.76.2.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yasuda S., Hirota Y. Cloning and mapping of the replication origin of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5458–5462. doi: 10.1073/pnas.74.12.5458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. von Meyenburg K., Hansen F. G., Nielsen L. D., Jørgensen P. Origin of replication, oriC, of the Escherichia coli chromosome: mapping of genes relative to R.EcoRI cleavage sites in the oriC region. Mol Gen Genet. 1977 Dec 14;158(1):101–109. doi: 10.1007/BF00455124. [DOI] [PubMed] [Google Scholar]
  25. von Meyenburg K., Hansen F. G., Riise E., Bergmans H. E., Meijer M., Messer W. Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):121–128. doi: 10.1101/sqb.1979.043.01.018. [DOI] [PubMed] [Google Scholar]

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