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. 1980 Sep;143(3):1275–1280. doi: 10.1128/jb.143.3.1275-1280.1980

Pyridine nucleotide control and subunit structure of phosphoribulokinase from photosynthetic bacteria.

F R Tabita
PMCID: PMC294495  PMID: 6251028

Abstract

With one exception, phosphoribulokinase from the Rhodospirillaceae requires reduced nicotinamide adenine dinucleotide for maximum activity. This mode of regulation is unique to the facultatively anaerobic photoorganotrophic photosynthetic bacteria, since the phosphoribulokinase from oxygen-evolving photosynthetic species is not subject to activation by reduced nicotinamide adenine dinucleotide. The enzyme was purified of fructose bisphosphatase activity from Rhodopseudomonas capsulata by means of affinity chromatography and was shown to have a native molecular weight of about 220,000. The homogeneous enzyme is composed of a single size polypeptide of 36,000 molecular weight. This study represents the first time the subunit structure of phosphoribulokinase has been determined from any source.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdelal A. T., Schlegel H. G. Purification and regulatory properties of phosphoribulokinase from Hydrogenomonas eutropha H 16. Biochem J. 1974 Jun;139(3):481–489. doi: 10.1042/bj1390481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abdelal A. T., Schlegel H. G. Separation of phosphoribulokinase from enzymes of the Calvin cycle in Hydrogenomonas eutropha H 16. Arch Mikrobiol. 1974 Feb 1;95(2):139–143. doi: 10.1007/BF02451755. [DOI] [PubMed] [Google Scholar]
  3. Gibson J. L., Tabita F. R. Different molecular forms of D-ribulose-1,5-bisphosphate carboxylase from Rhodopseudomonas sphaeroides. J Biol Chem. 1977 Feb 10;252(3):943–949. [PubMed] [Google Scholar]
  4. Gibson J. L., Tabita F. R. Isolation and preliminary characterization of two forms of ribulose 1,5-bisphosphate carboxylase from Rhodopseudomonas capsulata. J Bacteriol. 1977 Dec;132(3):818–823. doi: 10.1128/jb.132.3.818-823.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hart B. A., Gibson J. Ribulose-5-phosphate kinase from Chromatium sp. strain D. Arch Biochem Biophys. 1971 May;144(1):308–321. doi: 10.1016/0003-9861(71)90483-8. [DOI] [PubMed] [Google Scholar]
  6. Ihlenfeldt M. J., Gibson J. CO2 fixation and its regulation in Anacystis nidulans (Synechococcus). Arch Microbiol. 1975;102(1):13–21. doi: 10.1007/BF00428339. [DOI] [PubMed] [Google Scholar]
  7. Joint I. R., Morris I., Fuller R. C. Purification of a complex of alkaline fructose 1,6-bisphosphatase and phosphoribulokinase from Rhodospirillum rubrum. J Biol Chem. 1972 Aug 10;247(15):4833–4838. [PubMed] [Google Scholar]
  8. Kiesow L. A., Lindsley B. F., Bless J. W. Phosphoribulokinase from Nitrobacter winogradskyi: activation by reduced nicotinamide adenine dinucleotide and inhibition by pyridoxal phosphate. J Bacteriol. 1977 Apr;130(1):20–25. doi: 10.1128/jb.130.1.20-25.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
  11. MacElroy R. D., Johnson E. J., Johnson M. K. Control of ATP-dependent CO2 fixation in extracts of Hydrogenomonas facilis: NADH regulation of phosphoribulokinase. Arch Biochem Biophys. 1969 Apr;131(1):272–275. doi: 10.1016/0003-9861(69)90131-3. [DOI] [PubMed] [Google Scholar]
  12. MacElroy R. D., Mack H. M., Johnson E. J. Properties of phosphoribulokinase from Thiobacillus neapolitanus. J Bacteriol. 1972 Oct;112(1):532–538. doi: 10.1128/jb.112.1.532-538.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ORMEROD J. G., ORMEROD K. S., GEST H. Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys. 1961 Sep;94:449–463. doi: 10.1016/0003-9861(61)90073-x. [DOI] [PubMed] [Google Scholar]
  14. Orr M. D., Blakley R. L., Panagou D. Discontinuous buffer systems for analytical and preparative electrophoresis of enzymes on polyacrylamide gel. Anal Biochem. 1972 Jan;45(1):68–85. doi: 10.1016/0003-2697(72)90008-5. [DOI] [PubMed] [Google Scholar]
  15. Rindt K. P., Ohmann E. NADH and AMP as allosteric effectors of ribulose-5-phosphate kinase in Rhodopseudomonas spheroides. Biochem Biophys Res Commun. 1969 Aug 7;36(3):357–364. doi: 10.1016/0006-291x(69)90572-5. [DOI] [PubMed] [Google Scholar]
  16. Schroeder W. A., Shelton J. R., Shelton J. B., Robberson B., Apell G. The amino acid sequence of bovine liver catalase: a preliminary report. Arch Biochem Biophys. 1969 May;131(2):653–655. doi: 10.1016/0003-9861(69)90441-x. [DOI] [PubMed] [Google Scholar]
  17. Tabita F. R., Caruso P., Whitman W. Facile assay of enzymes unique to the Calvin cycle in intact cells, with special reference to ribulose 1,5-bisphosphate carboxylase. Anal Biochem. 1978 Feb;84(2):462–472. doi: 10.1016/0003-2697(78)90064-7. [DOI] [PubMed] [Google Scholar]
  18. Tabita F. R., McFadden B. A. D-ribulose 1,5-diphosphate carboxylase from Rhodospirillum rubrum. I. Levels, purification, and effects of metallic ions. J Biol Chem. 1974 Jun 10;249(11):3453–3458. [PubMed] [Google Scholar]
  19. Tabita R. F., Stevens S. E., Jr, Quijano R. D-ribulose 1, 5-diphosphate carboxylase from blue-green algae. Biochem Biophys Res Commun. 1974 Nov 6;61(1):45–52. doi: 10.1016/0006-291x(74)90531-2. [DOI] [PubMed] [Google Scholar]
  20. Whitman W. B., Martin M. N., Tabita F. R. Activation and regulation of ribulose bisphosphate carboxylase-oxygenase in the absence of small subunits. J Biol Chem. 1979 Oct 25;254(20):10184–10189. [PubMed] [Google Scholar]
  21. Whitman W., Tabita F. R. Inhibition of D-ribulose 1,5-bisphosphate carboxylase by pyridoxal 5'-phosphate. Biochem Biophys Res Commun. 1976 Aug 23;71(4):1034–1039. doi: 10.1016/0006-291x(76)90758-0. [DOI] [PubMed] [Google Scholar]

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