Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Oct;144(1):185–191. doi: 10.1128/jb.144.1.185-191.1980

Further characterization of sfiA and sfiB mutations in Escherichia coli.

O Huisman, R D'Ari, J George
PMCID: PMC294617  PMID: 6998947

Abstract

The sfiA and sfiB mutations, originally isolated in thermoresistant ultraviolet-resistant revertants of a tif lon strain, also suppressed filamentation in tsl strains (mutated at the lexA locus). When deoxyribonucleic acid synthesis was arrested, however, sfi-independent filamentation occurred. Other SOS functions were not affected by sfiA and sfiB mutations; in particular, ultraviolet-induced repair and mutagenesis of bacterial deoxyribonucleic acid were normal, as was tsl-tif-induced synthesis of recA protein. Genetic studies (i) established the identity of map location of the sfiA and sulA loci, (ii) showed that the two sfiB mutations are recessive, and (iii) showed that of six independent sfiA mutations, three are recessive and three are dominant. One sfiB strain was shown to have a 6% growth disadvantage relative to a sfi+ or sfiA strain. It is proposed that the sfiA locus may define the structural gene of a hypothetical inducible SOS-associated division inhibitor.

Full text

PDF
186

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bailone A., Blanco M., Devoret R. E. coli K12 inf: a mutant deficient in prophage lambda induction and cell filamentation. Mol Gen Genet. 1975;136(4):291–307. doi: 10.1007/BF00341714. [DOI] [PubMed] [Google Scholar]
  3. Castellazzi M., George J., Buttin G. Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation. Mol Gen Genet. 1972;119(2):139–152. doi: 10.1007/BF00269133. [DOI] [PubMed] [Google Scholar]
  4. Castellazzi M., George J., Buttin G. [Prophage induction and cell division in E. coli. II. Linked (recA, zab) and unlinked (lex) suppressors of tif-1-mediated induction and filamentation]. Mol Gen Genet. 1972;119(2):153–174. doi: 10.1007/BF00269134. [DOI] [PubMed] [Google Scholar]
  5. Castellazzi M., Jacques M., George J. tif-Stimulated deoxyribonucleic acid repair in Escherichia coli K-12. J Bacteriol. 1980 Aug;143(2):703–709. doi: 10.1128/jb.143.2.703-709.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Donch J., Green M. H., Greenberg J. Interaction of the exr and lon genes in Escherichia coli. J Bacteriol. 1968 Nov;96(5):1704–1710. doi: 10.1128/jb.96.5.1704-1710.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gayda R. C., Yamamoto L. T., Markovitz A. Second-site mutations in capR (lon) strains of Escherichia coli K-12 that prevent radiation sensitivity and allow bacteriophage lambda to lysogenize. J Bacteriol. 1976 Sep;127(3):1208–1216. doi: 10.1128/jb.127.3.1208-1216.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. George J., Castellazzi M., Buttin G. Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif. Mol Gen Genet. 1975 Oct 22;140(4):309–332. [PubMed] [Google Scholar]
  9. Gottesman S., Zipser D. Deg phenotype of Escherichia coli lon mutants. J Bacteriol. 1978 Feb;133(2):844–851. doi: 10.1128/jb.133.2.844-851.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Green M. H., Greenberg J., Donch J. Effect of a recA gene on cell division and capsular polysaccharide production in a lon strain of Escherichia coli. Genet Res. 1969 Oct;14(2):159–162. doi: 10.1017/s0016672300001993. [DOI] [PubMed] [Google Scholar]
  11. Gudas L. J., Mount D. W. Identification of the recA (tif) gene product of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5280–5284. doi: 10.1073/pnas.74.12.5280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gudas L. J. The induction of protein X in DNA repair and cell division mutants of Escherichia coli. J Mol Biol. 1976 Jul 5;104(3):567–587. doi: 10.1016/0022-2836(76)90121-2. [DOI] [PubMed] [Google Scholar]
  13. HOWARD-FLANDERS P., SIMSON E., THERIOT L. THE EXCISION OF THYMINE DIMERS FROM DNA, FILAMENT FORMATION AND SENSITIVITY TO ULTRAVIOLET LIGHT IN ESCHERICHIA COLI K-12. Mutat Res. 1964 Oct;106:219–226. doi: 10.1016/0027-5107(64)90002-8. [DOI] [PubMed] [Google Scholar]
  14. Huisman O., D'Ari R., George J. Dissociation of tsl-tif-induced filamentation and recA protein synthesis in Escherichia coli K-12. J Bacteriol. 1980 Jun;142(3):819–828. doi: 10.1128/jb.142.3.819-828.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Huisman O., D'Ari R., George J. Inducible sfi dependent division inhibition in Escherichia coli. Mol Gen Genet. 1980;177(4):629–636. doi: 10.1007/BF00272673. [DOI] [PubMed] [Google Scholar]
  16. Johnson B. F. Fine structure mapping and properties of mutations suppressing the lon mutation in Escherichia coli K-12 and B strains. Genet Res. 1977 Dec;30(3):273–286. doi: 10.1017/s0016672300017687. [DOI] [PubMed] [Google Scholar]
  17. Little J. W., Edmiston S. H., Pacelli L. Z., Mount D. W. Cleavage of the Escherichia coli lexA protein by the recA protease. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3225–3229. doi: 10.1073/pnas.77.6.3225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mound D. W., Walker A. C., Kosel C. Suppression of lex mutations affecting deoxyribonucleic acid repair in Escherichia coli K-12 by closely linked thermosensitive mutations. J Bacteriol. 1973 Nov;116(2):950–956. doi: 10.1128/jb.116.2.950-956.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Otsuji N., Iyehara-Ogawa H. Thermoresistant revertants of an Escherichia coli strain carrying tif-1 and ruv mutations: non-suppressibility of ruv by sfi. J Bacteriol. 1979 Apr;138(1):1–6. doi: 10.1128/jb.138.1.1-6.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Radman M. SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. Basic Life Sci. 1975;5A:355–367. doi: 10.1007/978-1-4684-2895-7_48. [DOI] [PubMed] [Google Scholar]
  21. Satta G., Pardee A. B. Inhibition of Escherichia coli division by protein X. J Bacteriol. 1978 Mar;133(3):1492–1500. doi: 10.1128/jb.133.3.1492-1500.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Uzan M., Danchin A. Correlation between the serine sensitivity and the derepressibility of the ilv genes in Escherichia coli relA- mutants. Mol Gen Genet. 1978 Sep 20;165(1):21–30. doi: 10.1007/BF00270372. [DOI] [PubMed] [Google Scholar]
  23. Witkin E. M. Genetics of Resistance to Radiation in ESCHERICHIA COLI. Genetics. 1947 May;32(3):221–248. doi: 10.1093/genetics/32.3.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Witkin E. M. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976 Dec;40(4):869–907. doi: 10.1128/br.40.4.869-907.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES