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. 1980 Dec;144(3):1043–1047. doi: 10.1128/jb.144.3.1043-1047.1980

Temperature-sensitive glutamate dehydrogenase mutants of Salmonella typhimurium.

S M Dendinger, J E Brenchley
PMCID: PMC294769  PMID: 7002902

Abstract

Mutants of Salmonella typhimurium defective in glutamate dehydrogenase activity were isolated in parent strains lacking glutamate synthase activity by localizcd mutagenesis or by a general mutagenesis combined with a cycloserine enrichment for glutamate auxotrophs. Two mutants with temperature-sensitive phenotypes had glutamate dehydrogenase activities that were more thermolabile than that of an isogenic control strain. Eight other mutants had less than 10% of the wild-type glutamate dehydrogenase activity. All the mutations were cotransducible with a Tn10 element (zed-2:Tn10) located at approximately 23 U on the S. typhimurium linkage map. These data strongly indicate that this region contains the structural gene (gdhA) for glutamate dehydrogenase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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