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. 1995 Feb;95(2):611–618. doi: 10.1172/JCI117705

Effects of dietary cholesterol and fat saturation on plasma lipoproteins in an ethnically diverse population of healthy young men.

C J Fielding 1, R J Havel 1, K M Todd 1, K E Yeo 1, M C Schloetter 1, V Weinberg 1, P H Frost 1
PMCID: PMC295526  PMID: 7860745

Abstract

The individual effects of dietary cholesterol and fat saturation on plasma lipoprotein concentrations were determined in an ethnically diverse population of normolipidemic young men (52 Caucasian, 32 non-Caucasian). The experimental diets contained approximately 200 or 600 mg/d of cholesterol, 36-38% of calories as fat, and high or low proportions of saturated and polyunsaturated fat (polyunsaturated/saturated fat ratio approximately 0.8 vs 0.3). At the lower cholesterol intake, the high saturated fat diet had only a modest effect on LDL cholesterol in Caucasians (+ 6 mg/dl-1) and none in non-Caucasians. 600 mg cholesterol with high saturated fat led to a substantial mean increase in LDL cholesterol, which was significantly greater in Caucasian than in non-Caucasian subjects (+ 31 mg/dl vs 16 mg/dl, P < 0.005). 600 mg cholesterol with increased polyunsaturated fat gave a mean LDL increase of 16 mg/dl, lower than found when the same high cholesterol intake was coupled with increased saturated fat. Variation in cholesterol rather than the proportions of saturated and polyunsaturated fat had the most influence on LDL-cholesterol levels. Among non-Caucasians it was the only significant factor.

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Selected References

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  1. Abbey M., Clifton P., Kestin M., Belling B., Nestel P. Effect of fish oil on lipoproteins, lecithin:cholesterol acyltransferase, and lipid transfer protein activity in humans. Arteriosclerosis. 1990 Jan-Feb;10(1):85–94. doi: 10.1161/01.atv.10.1.85. [DOI] [PubMed] [Google Scholar]
  2. Allain C. C., Poon L. S., Chan C. S., Richmond W., Fu P. C. Enzymatic determination of total serum cholesterol. Clin Chem. 1974 Apr;20(4):470–475. [PubMed] [Google Scholar]
  3. Applebaum-Bowden D., Haffner S. M., Hartsook E., Luk K. H., Albers J. J., Hazzard W. R. Down-regulation of the low-density lipoprotein receptor by dietary cholesterol. Am J Clin Nutr. 1984 Mar;39(3):360–367. doi: 10.1093/ajcn/39.3.360. [DOI] [PubMed] [Google Scholar]
  4. Bucolo G., David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem. 1973 May;19(5):476–482. [PubMed] [Google Scholar]
  5. Castro G. R., Fielding C. J. Effects of postprandial lipemia on plasma cholesterol metabolism. J Clin Invest. 1985 Mar;75(3):874–882. doi: 10.1172/JCI111786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen J. C. Chylomicron triglyceride clearance: comparison of three assessment methods. Am J Clin Nutr. 1989 Feb;49(2):306–313. doi: 10.1093/ajcn/49.2.306. [DOI] [PubMed] [Google Scholar]
  7. Derr J., Kris-Etherton P. M., Pearson T. A., Seligson F. H. The role of fatty acid saturation on plasma lipids, lipoproteins, and apolipoproteins: II. The plasma total and low-density lipoprotein cholesterol response of individual fatty acids. Metabolism. 1993 Jan;42(1):130–134. doi: 10.1016/0026-0495(93)90183-o. [DOI] [PubMed] [Google Scholar]
  8. Fielding C. J. Lecithin-cholesterol acyltransferase and cholesterol transport. Methods Enzymol. 1985;111:267–274. doi: 10.1016/s0076-6879(85)11014-1. [DOI] [PubMed] [Google Scholar]
  9. Fielding P. E., Ishikawa Y., Fielding C. J. Apolipoprotein E mediates binding of normal very low density lipoprotein to heparin but is not required for high affinity receptor binding. J Biol Chem. 1989 Jul 25;264(21):12462–12466. [PubMed] [Google Scholar]
  10. Grundy S. M., Denke M. A. Dietary influences on serum lipids and lipoproteins. J Lipid Res. 1990 Jul;31(7):1149–1172. [PubMed] [Google Scholar]
  11. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hayes K. C., Khosla P. Dietary fatty acid thresholds and cholesterolemia. FASEB J. 1992 May;6(8):2600–2607. doi: 10.1096/fasebj.6.8.1592210. [DOI] [PubMed] [Google Scholar]
  13. Hegsted D. M., Ausman L. M., Johnson J. A., Dallal G. E. Dietary fat and serum lipids: an evaluation of the experimental data. Am J Clin Nutr. 1993 Jun;57(6):875–883. doi: 10.1093/ajcn/57.6.875. [DOI] [PubMed] [Google Scholar]
  14. Hegsted D. M., McGandy R. B., Myers M. L., Stare F. J. Quantitative effects of dietary fat on serum cholesterol in man. Am J Clin Nutr. 1965 Nov;17(5):281–295. doi: 10.1093/ajcn/17.5.281. [DOI] [PubMed] [Google Scholar]
  15. Hopkins P. N. Effects of dietary cholesterol on serum cholesterol: a meta-analysis and review. Am J Clin Nutr. 1992 Jun;55(6):1060–1070. doi: 10.1093/ajcn/55.6.1060. [DOI] [PubMed] [Google Scholar]
  16. Kestin M., Clifton P. M., Rouse I. L., Nestel P. J. Effect of dietary cholesterol in normolipidemic subjects is not modified by nature and amount of dietary fat. Am J Clin Nutr. 1989 Sep;50(3):528–532. doi: 10.1093/ajcn/50.3.528. [DOI] [PubMed] [Google Scholar]
  17. Kesäniemi Y. A., Beltz W. F., Grundy S. M. Comparisons of metabolism of apolipoprotein B in normal subjects, obese patients, and patients with coronary heart disease. J Clin Invest. 1985 Aug;76(2):586–595. doi: 10.1172/JCI112010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kostner G. M. Letter: Enzymatic determination of cholesterol in high-density lipoprotein fractions prepared by polyanion precipitation. Clin Chem. 1976 May;22(5):695–695. [PubMed] [Google Scholar]
  19. Lin E. C., Fernandez M. L., Tosca M. A., McNamara D. J. Regulation of hepatic LDL metabolism in the guinea pig by dietary fat and cholesterol. J Lipid Res. 1994 Mar;35(3):446–457. [PubMed] [Google Scholar]
  20. Mann C. J., Yen F. T., Grant A. M., Bihain B. E. Mechanism of plasma cholesteryl ester transfer in hypertriglyceridemia. J Clin Invest. 1991 Dec;88(6):2059–2066. doi: 10.1172/JCI115535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Marcel Y. L., Vezina C. Lecithin: cholesterol acyltransferase of human plasma. Role of chylomicrons, very low, and high density lipoproteins in the reaction. J Biol Chem. 1973 Dec 10;248(23):8254–8259. [PubMed] [Google Scholar]
  22. Mistry P., Miller N. E., Laker M., Hazzard W. R., Lewis B. Individual variation in the effects of dietary cholesterol on plasma lipoproteins and cellular cholesterol homeostasis in man. Studies of low density lipoprotein receptor activity and 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in blood mononuclear cells. J Clin Invest. 1981 Feb;67(2):493–502. doi: 10.1172/JCI110058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ng T. K., Hayes K. C., DeWitt G. F., Jegathesan M., Satgunasingam N., Ong A. S., Tan D. Dietary palmitic and oleic acids exert similar effects on serum cholesterol and lipoprotein profiles in normocholesterolemic men and women. J Am Coll Nutr. 1992 Aug;11(4):383–390. doi: 10.1080/07315724.1992.10718241. [DOI] [PubMed] [Google Scholar]
  24. Ogawa Y., Fielding C. J. Assay of cholesteryl ester transfer activity and purification of a cholesteryl ester transfer protein. Methods Enzymol. 1985;111:274–285. doi: 10.1016/s0076-6879(85)11015-3. [DOI] [PubMed] [Google Scholar]
  25. Packard C. J., McKinney L., Carr K., Shepherd J. Cholesterol feeding increases low density lipoprotein synthesis. J Clin Invest. 1983 Jul;72(1):45–51. doi: 10.1172/JCI110983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Phillips N. R., Havel R. J., Kane J. P. Levels and interrelationships of serum and lipoprotein cholesterol and triglycerides. Association with adiposity and the consumption of ethanol, tobacco, and beverages containing caffeine. Arteriosclerosis. 1981 Jan-Feb;1(1):13–24. doi: 10.1161/01.atv.1.1.13. [DOI] [PubMed] [Google Scholar]
  27. Quinet E., Tall A., Ramakrishnan R., Rudel L. Plasma lipid transfer protein as a determinant of the atherogenicity of monkey plasma lipoproteins. J Clin Invest. 1991 May;87(5):1559–1566. doi: 10.1172/JCI115169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schonfeld G., Patsch W., Rudel L. L., Nelson C., Epstein M., Olson R. E. Effects of dietary cholesterol and fatty acids on plasma lipoproteins. J Clin Invest. 1982 May;69(5):1072–1080. doi: 10.1172/JCI110542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Spady D. K., Woollett L. A., Dietschy J. M. Regulation of plasma LDL-cholesterol levels by dietary cholesterol and fatty acids. Annu Rev Nutr. 1993;13:355–381. doi: 10.1146/annurev.nu.13.070193.002035. [DOI] [PubMed] [Google Scholar]
  30. Tall A., Sammett D., Granot E. Mechanisms of enhanced cholesteryl ester transfer from high density lipoproteins to apolipoprotein B-containing lipoproteins during alimentary lipemia. J Clin Invest. 1986 Apr;77(4):1163–1172. doi: 10.1172/JCI112417. [DOI] [PMC free article] [PubMed] [Google Scholar]

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