Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1967 Mar;46(3):400–408. doi: 10.1172/JCI105541

Studies of Cryoproteins in Systemic Lupus Erythematosus*

Lonnie B Hanauer 1,†, Charles L Christian 1,‡
PMCID: PMC297060  PMID: 4164677

Abstract

Cryoproteins observed in patients with systemic lupus erythematosus were shown to be of “mixed type,” consisting largely of IgG and IgM. The IgM moiety possessed anti-IgG globulin reactivity and precipitated in the cold after mixing with a source of IgG. The IgM fraction of one cryoprotein precipitated only with the same patient's IgG. Antisera prepared against purified cryoproteins regularly recognized IgM, IgG, the 11 S component of C′, and α2-macroglobulin. C′4 (β1E) and C′3 (β1C) were recognized by four and two antisera, respectively. Antisera prepared against two cryoproteins reacted (after absorption) only with the sera of origin. These “unique” antigens were associated with IgM, were destroyed by mercaptoethanol treatment, and, in one patient, were shown to disappear subsequent to clinical improvement.

Full text

PDF
405

Images in this article

403Image
on p.403
403Image
on p.403
404Image
on p.404
404Image
on p.404
405Image
on p.405

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLLEN J. C., KUNKEL H. G., KABAT E. A. STUDIES ON HUMAN ANTIBODIES. II. DISTRIBUTION OF GENETIC FACTORS. J Exp Med. 1964 Mar 1;119:453–465. doi: 10.1084/jem.119.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHRISTIAN C. L., HATFIELD W. B., CHASE P. H. Systemic lupus erythematosus. Cryoprecipitation of sera. J Clin Invest. 1963 Jun;42:823–829. doi: 10.1172/JCI104774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COSTANZI J. J., COLTMAN C. A., Jr, CLARK D. A., TENNENBAUM J. I., CRISCUOLO D. CRYOBLOBULINEMIA ASSOCIATED WITH A MACROGLOBULIN; STUDIES OF A 17.5S CRYOPRECIPITATING FACTOR. Am J Med. 1965 Jul;39:163–172. doi: 10.1016/0002-9343(65)90256-1. [DOI] [PubMed] [Google Scholar]
  4. ELLIS H. A., FELIX-DAVIES D. Serum complement, rheumatoid factor, and other serum proteins in rheumatoid disease and systemic lupus erythematosus. Ann Rheum Dis. 1959 Sep;18:215–224. doi: 10.1136/ard.18.3.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GELL P. G., KELUS A. Deletions of allotypic gamma-globulins in antibodies. Nature. 1962 Jul 7;195:44–45. doi: 10.1038/195044a0. [DOI] [PubMed] [Google Scholar]
  6. GRABAR P., WILLIAMS C. A. Méthode permettant l'étude conjuguée des proprietés électrophorétiques et immunochimiques d'un mélange de protéines; application au sérum sanguin. Biochim Biophys Acta. 1953 Jan;10(1):193–194. doi: 10.1016/0006-3002(53)90233-9. [DOI] [PubMed] [Google Scholar]
  7. HARBOE M., DEVERILL J., GODAL H. C. ANTIGENIC HETEROGENEITY OF WALDENSTROEM TYPE GAMMA-M-GLOBULINS. Scand J Haematol. 1965;2:137–147. [PubMed] [Google Scholar]
  8. HARBOE M. Interactions between red cells coated with incomplete anti-D and rheumatoid sera. Acta Pathol Microbiol Scand. 1960;50:383–397. doi: 10.1111/j.1699-0463.1960.tb01208.x. [DOI] [PubMed] [Google Scholar]
  9. Heidelberger M., Macpherson C. F. QUANTITATIVE MICRO-ESTIMATION OF ANTIBODIES IN THE SERA OF MAN AND OTHER ANIMALS. Science. 1943 Apr 30;97(2522):405–406. doi: 10.1126/science.97.2522.405. [DOI] [PubMed] [Google Scholar]
  10. LEPOW I. H., NAFF G. B., TODD E. W., PENSKY J., HINZ C. F. Chromatographic resolution of the first component of human complement into three activities. J Exp Med. 1963 Jun 1;117:983–1008. doi: 10.1084/jem.117.6.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOSPALLUTO J., DORWARD B., MILLER W., Jr, ZIFF M. Cryoglobulinemia based on interaction between a gamma macroglobulin and 7S gamma globulin. Am J Med. 1962 Jan;32:142–147. doi: 10.1016/0002-9343(62)90191-2. [DOI] [PubMed] [Google Scholar]
  12. MORSE J. H., MULLER-EBERHARD H. J., KUNKEL H. G. Anti-nuclear factors and serum complement in systemic lupus erythematosus. Bull N Y Acad Med. 1962 Oct;38:641–651. [PMC free article] [PubMed] [Google Scholar]
  13. Meltzer M., Franklin E. C. Cryoglobulinemia--a study of twenty-nine patients. I. IgG and IgM cryoglobulins and factors affecting cryoprecipitability. Am J Med. 1966 Jun;40(6):828–836. doi: 10.1016/0002-9343(66)90199-9. [DOI] [PubMed] [Google Scholar]
  14. Meltzer M., Franklin E. C., Elias K., McCluskey R. T., Cooper N. Cryoglobulinemia--a clinical and laboratory study. II. Cryoglobulins with rheumatoid factor activity. Am J Med. 1966 Jun;40(6):837–856. doi: 10.1016/0002-9343(66)90200-2. [DOI] [PubMed] [Google Scholar]
  15. PEETOOM F., LOGHEM-LANGEREIS E. IGM-IGG (BETA-2M-7S-GAMMA) CRYOGLOBULINAEMIA AN AUTO-IMMUNE PHENOMEMON. Vox Sang. 1965 May-Jun;10:281–292. doi: 10.1111/j.1423-0410.1965.tb01391.x. [DOI] [PubMed] [Google Scholar]
  16. RIEDER R. F., OUDIN J. STUDIES ON THE RELATIONSHIP OF ALLOTYPIC SPECIFICITIES TO ANTIBODY SPECIFICITIES IN THE RABBIT. J Exp Med. 1963 Oct 1;118:627–633. doi: 10.1084/jem.118.4.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. VAUGHAN J. H., BAYLES T. B., FAVOUR C. B. The response of serum gamma globulin level and complement titer to adrenocorticotropic hormone therapy in lupus erythematosus disseminatus. J Lab Clin Med. 1951 May;37(5):698–702. [PubMed] [Google Scholar]
  18. WASSERMAN E., LEVINE L. Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition. J Immunol. 1961 Sep;87:290–295. [PubMed] [Google Scholar]
  19. WILLIAMS R. C., Jr, LAW D. H., 4th Serum complement in connective tissue disorders. J Lab Clin Med. 1958 Aug;52(2):273–281. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES