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. 1967 Aug;46(8):1329–1337. doi: 10.1172/JCI105625

A Low Molecular Weight Immunoglobulin Antigenically Related to 19 S IgM*

John D Stobo 1, Thomas B Tomasi Jr 1,†
PMCID: PMC297134  PMID: 16695921

Abstract

Sucrose density gradient analysis of the fresh sera of patients with hereditary ataxia telangiectasia, disseminated lupus, and Waldenström's macroglobulinemia revealed the presence of an immunoglobulin possessing IgM determinants but having a sedimentation coefficient of approximately 7 S. Bio-Gel chromatography of patients' sera confirmed the presence of two distinct populations of IgM. The low molecular weight IgM possessed incomplete isohemagglutinin activity that was resistant to treatment with reducing agents. Gel diffusion analysis revealed that the 7 S IgM showed immunological identity with both 19 S IgM and the subunits of the 19 S IgM produced by reduction. Approximately 10 to 15% of the patient's total IgM was low molecular weight. Evidence is presented that the 7 S IgM was not produced from the patient's serum 19 S IgM on in vitro incubation. A simple rapid technique is described, using double diffusion in polyacrylamide gels, which permits the determination of low molecular weight IgM in sera and other fluids. Using this technique, the sera of 52 patients with disseminated lupus were surveyed, and 17% of the patients were found to contain low molecular weight IgM. The low molecular weight IgM occurred with particular frequency in male patients with disseminated lupus and in those patients with low or absent serum IgA.

Studies of the salivary immunoglobulins of patients with ataxia telangiectasia and disseminated lupus suggest an iverse relationship between the levels of IgA and IgM. In patients lacking salivary IgA, IgM was the major immunoglobulin present. No correlation was observed between salivary immunoglobulin levels and the severity of sinopulmonary infections in these patients.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHAN P. C., DEUTSCH H. F. Immunochemical studies of human serum Rh agglutinins. J Immunol. 1960 Jul;85:37–45. [PubMed] [Google Scholar]
  2. Clem L. W., Small P. A., Jr Phylogeny of immunoglobulin structure and function. I. Immunoglobulins of the lemon shark. J Exp Med. 1967 May 1;125(5):893–920. doi: 10.1084/jem.125.5.893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DODD B. E., WILKINSON P. C. A STUDY OF THE DISTRIBUTION OF INCOMPLETE RHESUS ANTIBODIES AMONG THE SERUM IMMUNOGLOBULIN FRACTIONS. J Exp Med. 1964 Jul 1;120:45–55. doi: 10.1084/jem.120.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FINSTAD J., PAPERMASTER B. W., GOOD R. A. EVOLUTION OF THE IMMUNE RESPONSE. II. MORPHOLOGIC STUDIES ON THE ORIGIN OF THE THYMUS AND ORGANIZED LYMPHOID TISSUE. Lab Invest. 1964 May;13:490–512. [PubMed] [Google Scholar]
  5. GALBRAITH R. F., SUMMERSKILL W. H., MURRAY J. SYSTEMIC LUPUS ERYTHEMATOSUS, CIRRHOSIS AND ULCERATIVE COLITIS AFTER THYMECTOMY FOR MYASTHENIA GRAVIS. N Engl J Med. 1964 Jan 30;270:229–231. doi: 10.1056/NEJM196401302700504. [DOI] [PubMed] [Google Scholar]
  6. Gleich G. J., Uhr J. W., Vaughan J. H., Swedlund H. A. Antibody formation in dysgammaglobulinemia. J Clin Invest. 1966 Aug;45(8):1334–1340. doi: 10.1172/JCI105440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Good R. A., Peterson R. D., Martinez C., Sutherland D. E., Kellum M. J., Finstad J. The thymus in immunobiology: with special reference to autoimmune disease. Ann N Y Acad Sci. 1965 Jun 30;124(1):73–94. doi: 10.1111/j.1749-6632.1965.tb18944.x. [DOI] [PubMed] [Google Scholar]
  8. JACOT-GUILLARMOD H., ISLIKER H. SCISSION R'EVERSIBLE DES ISOAGGLUTININES 19 S: ETUDE DE FIXATION DES SUBUNIT'ES. Vox Sang. 1964 Jan-Feb;9:31–35. doi: 10.1111/j.1423-0410.1964.tb03764.x. [DOI] [PubMed] [Google Scholar]
  9. KILLANDER J. SEPARATION OF HUMAN IMMUNOGLOBULINS BY GEL FILTRATION AND ZONE ELECTROPHORESIS. Acta Soc Med Ups. 1963;68:230–244. [PubMed] [Google Scholar]
  10. MILLER F., METZGER H. CHARACTERIZATION OF A HUMAN MACROGLOBULIN. I. THE MOLECULAR WEIGHT OF ITS SUBUNIT. J Biol Chem. 1965 Aug;240:3325–3333. [PubMed] [Google Scholar]
  11. Marchalonis J., Edelman G. M. Phylogenetic origins of antibody structure. I. Multichain structure of immunoglobulins in the smooth dogfish (Mustelus canis). J Exp Med. 1965 Sep 1;122(3):601–618. doi: 10.1084/jem.122.3.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McFarlin D. E., Strober W., Wochner R. D., Waldmann T. A. Immunoglobulin A production in ataxia telangiectasia. Science. 1965 Nov 26;150(3700):1175–1177. doi: 10.1126/science.150.3700.1175. [DOI] [PubMed] [Google Scholar]
  13. PETERSON R. D., KELLY W. D., GOOD R. A. ATAXIA-TELANGIECTASIA. ITS ASSOCIATION WITH A DEFECTIVE THYMUS, IMMUNOLOGICAL-DEFICIENCY DISEASE, AND MALIGNANCY. Lancet. 1964 May 30;1(7344):1189–1193. doi: 10.1016/s0140-6736(64)91209-7. [DOI] [PubMed] [Google Scholar]
  14. ROCKEY J. H., HANSON L. A., HEREMANS J. F., KUNKEL H. G. BETA-2A AGLOBULINEMIA IN TWO HEALTHY MEN. J Lab Clin Med. 1964 Feb;63:205–212. [PubMed] [Google Scholar]
  15. ROTHFIELD N. F., FRANGIONE B., FRANKLIN E. C. SLOWLY SEDIMENTING MERCAPTOETHANOL-RESISTANT ANTINUCLEAR FACTORS RELATED ANTIGENICALLY TO M IMMUNOGLOBULINS (GAMMA-1M-GLOBULIN) IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS. J Clin Invest. 1965 Jan;44:62–72. doi: 10.1172/JCI105127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SANDOR G., KORACH S., MATTERN P. 7S GLOBULIN, IMMUNOLOGICALLY IDENTICAL TO 19S GAMMA-1 (BETA-2)-M-GLOBULIN, A NEW PROTEIN OR HORSE SERUM. Nature. 1964 Nov 21;204:795–796. doi: 10.1038/204795a0. [DOI] [PubMed] [Google Scholar]
  17. SCHROHENLOHER R. E., KUNKEL H. G., TOMASI T. B. ACTIVITY OF DISSOCIATED AND REASSOCIATED 19S ANTI-GAMMA-GLOBULINS. J Exp Med. 1964 Dec 1;120:1215–1229. doi: 10.1084/jem.120.6.1215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. TOMASI T. B., Jr, ZIGELBAUM S. THE SELECTIVE OCCURENCE OF GAMMA-1A GLOBULINS IN CERTAIN BODY FLUIDS. J Clin Invest. 1963 Oct;42:1552–1560. doi: 10.1172/JCI104840. [DOI] [PMC free article] [PubMed] [Google Scholar]

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