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. 1968 May;47(5):1154–1161. doi: 10.1172/JCI105804

Effect of prostaglandin E1 on the permeability response of the isolated collecting tubule to vasopressin, adenosine 3′,5′-monophosphate, and theophylline

Jared J Grantham 1, Jack Orloff 1
PMCID: PMC297267  PMID: 4296382

Abstract

The effect of prostaglandin E1 (PGE1) on the water permeability response to vasopressin, theophylline, and cyclic adenosine 3′,5′-monophosphate (C-AMP) of isolated, perfused collecting tubules of the rabbit was investigated in vitro. Prostaglandin is a naturally occurring substance present in a number of tissues, including kidney. It has been implicated in the action of a variety of hormones, many of which are known to exert their physiological effects through the intermediacy of the C-AMP system. In the collecting tubule, PGE1 (10-7 M) elicited a minimal increase in net water absorption along an osmotic gradient. However, when administered in association with a concentration of vasopressin (2.5 μU ml-1) selected to induce a submaximal increment in water absorption, the effect of the latter was reduced by approximately 50%. Theophylline (5 × 10-3 M) also increased net water absorption, an effect not previously demonstrated in renal tissue. This effect was potentiated by the simulataneous addition of PGE1. In contrast, PGE1 did not influence the increase in net water absorption induced by C-AMP (10-2 M). Since C-AMP is responsible for the permeability effects of vasopressin in renal tissue, the present results are consistent with the view that PGE1 interferes with the action of the octapeptide by competing with it at a site which influences the generation of C-AMP. In addition it is proposed that prostaglandin may be an important modulator of the action of vasopressin. The tubule is exquisitely sensitive to the hormone, responding to as little as 0.25 μU ml-1. It is conceivable that in the intact animal prostaglandin may serve to dampen the effects of small amounts of residual hormone and thereby prevent overshoots in permeability which might otherwise occur.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burg M., Grantham J., Abramow M., Orloff J. Preparation and study of fragments of single rabbit nephrons. Am J Physiol. 1966 Jun;210(6):1293–1298. doi: 10.1152/ajplegacy.1966.210.6.1293. [DOI] [PubMed] [Google Scholar]
  2. Butcher R. W., Sutherland E. W. The effects of the catecholamines, adrenergic blocking agents, prostaglandin E1, and insulin on cyclie AMP levels in the rat epididymal fat pad in vitro. Ann N Y Acad Sci. 1967 Feb 10;139(3):849–859. doi: 10.1111/j.1749-6632.1967.tb41255.x. [DOI] [PubMed] [Google Scholar]
  3. Grantham J. J., Burg M. B. Effect of vasopressin and cyclic AMP on permeability of isolated collecting tubules. Am J Physiol. 1966 Jul;211(1):255–259. doi: 10.1152/ajplegacy.1966.211.1.255. [DOI] [PubMed] [Google Scholar]
  4. Handler J. S., Butcher R. W., Sutherland E. W., Orloff J. The effect of vasopressin and of theophylline on the concentration of adenosine 3',5'-phosphate in the urinary bladder of the toad. J Biol Chem. 1965 Nov;240(11):4524–4526. [PubMed] [Google Scholar]
  5. LEE J. B., COVINO B. G., TAKMAN B. H., SMITH E. R. RENOMEDULLARY VASODEPRESSOR SUBSTANCE, MEDULLIN: ISOLATION, CHEMICAL CHARACTERIZATION AND PHYSIOLOGICAL PROPERTIES. Circ Res. 1965 Jul;17:57–77. doi: 10.1161/01.res.17.1.57. [DOI] [PubMed] [Google Scholar]
  6. Lauson H. D. Metabolism of antidiuretic hormones. Am J Med. 1967 May;42(5):713–744. doi: 10.1016/0002-9343(67)90091-5. [DOI] [PubMed] [Google Scholar]
  7. ORLOFF J., HANDLER J. S., BERGSTROM S. EFFECT OF PROSTAGLANDIN (PGE-1) ON THE PERMEABILITY RESPONSE OF TOAD BLADDER TO VASOPRESSIN, THEOPHYLLINE AND ADENOSINE 3',5'-MONOPHOSPHATE. Nature. 1965 Jan 23;205:397–398. doi: 10.1038/205397a0. [DOI] [PubMed] [Google Scholar]
  8. ORLOFF J., HANDLER J. S. The similarity of effects of vasopressin, adenosine-3',5'-phosphate (cyclic AMP) and theophylline on the toad bladder. J Clin Invest. 1962 Apr;41:702–709. doi: 10.1172/JCI104528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. STEINBERG D., VAUGHAN M., NESTEL P. J., STRAND O., BERGSTROEM S. EFFECTS OF THE PROSTAGLANDINS ON HORMONE-INDUCED MOBILIZATION OF FREE FATTY ACIDS. J Clin Invest. 1964 Aug;43:1533–1540. doi: 10.1172/JCI105030. [DOI] [PMC free article] [PubMed] [Google Scholar]

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