Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1968 May;47(5):1162–1171. doi: 10.1172/JCI105805

Secretory γA in normal urine

John Bienenstock 1, Thomas B Tomasi Jr 1
PMCID: PMC297268  PMID: 4171452

Abstract

The physicochemical nature of γA was investigated in normal male and female urine concentrated approximately 1000 times. Sucrose density gradient ultracentrifugation and Sephadex G-200 chromatography revealed that urinary γA has sedimentation properties intermediate between 19S and 7S molecules. Isolation of urinary γA by DE 52 chromatography free of other immunoglobulins with subsequent antigenic analysis showed that the urinary γA-molecule is antigenically indistinguishable from the γA-molecules found in other external secretions and has a corrected sedimentation coefficient of 11.8S. In addition, like other secretory γA-molecules and unlike serum polymeric γA, urinary γA resisted mild reductive measures with 0.1M β-mercaptoethanol. Free or unattached secretory “piece” was found in all normal urines tested and in agammaglobulinemic urine. Secretory “piece” antigenic determinants were also found in ureteric urine. The average daily excretion of urinary γA was 1.1 mg. The maximum excretion of urinary 7S γG per 24 hr was approximately 3 mg.

Full text

PDF
1165

Images in this article

1164Image
on p.1164
1164Image
on p.1164
1164Image
on p.1164
1165Image
on p.1165
1165Image
on p.1165
1166Image
on p.1166
1166Image
on p.1166

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger R., Ainbender E., Hodes H. L., Zepp H. D., Hevizy M. M. Demonstration of IgA polioantibody in saliva, duodenal fluid and urine. Nature. 1967 Apr 22;214(5086):420–422. doi: 10.1038/214420a0. [DOI] [PubMed] [Google Scholar]
  2. Berggård I., Bennich H. Fc fragment of immunoglobulin G in normal human plasma and urine. Nature. 1967 May 13;214(5089):697–699. doi: 10.1038/214697a0. [DOI] [PubMed] [Google Scholar]
  3. Bienenstock J., Bloch K. J. Some characteristics of human immunoconglutinin. J Immunol. 1966 Apr;96(4):637–645. [PubMed] [Google Scholar]
  4. CHODIRKER W. B., TOMASI T. B., Jr GAMMA-GLOBULINS: QUANTITATIVE RELATIONSHIPS IN HUMAN SERUM AND NONVASCULAR FLUIDS. Science. 1963 Nov 22;142(3595):1080–1081. doi: 10.1126/science.142.3595.1080. [DOI] [PubMed] [Google Scholar]
  5. EVERALL P. H., WRIGHT G. H. Low pressure ultra-filtration of protein-containing fluids. J Med Lab Technol. 1958 Jul;15(3):209–213. [PubMed] [Google Scholar]
  6. FRANKLIN E. C. Physicochemical and immunologic studies of gamma globulins of normal human urine. J Clin Invest. 1959 Dec;38:2159–2167. doi: 10.1172/JCI103995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HANSON L. A., TAN E. M. CHARACTERIZATION OF ANTIBODIES IN HUMAN URINE. J Clin Invest. 1965 May;44:703–715. doi: 10.1172/JCI105183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Miyasato F., Pollak V. E. Serum proteins in urine: an examination of the effects of some methods used to concentrate the urine. J Lab Clin Med. 1966 Jun;67(6):1036–1043. [PubMed] [Google Scholar]
  10. Poortmans J., Van Fraechem J. Influence de certaines techniques de conservation et de concentration des urines visa-vis de l'intégrité des protéines plasmatiques. Clin Chim Acta. 1965 Jul;12(1):114–115. doi: 10.1016/0009-8981(65)90117-8. [DOI] [PubMed] [Google Scholar]
  11. Poulik M. D. F'c fragment of immunoglobulins. Nature. 1966 Apr 9;210(5032):133–134. doi: 10.1038/210133a0. [DOI] [PubMed] [Google Scholar]
  12. SOLOMON A., WALDMANN T. A., FAHEY J. L., MCFARLANE A. S. METABOLISM OF BENCE JONES PROTEINS. J Clin Invest. 1964 Jan;43:103–117. doi: 10.1172/JCI104884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. South M. A., Cooper M. D., Wollheim F. A., Hong R., Good R. A. The IgA system. I. Studies of the transport and immunochemistry of IgA in the saliva. J Exp Med. 1966 Apr 1;123(4):615–627. doi: 10.1084/jem.123.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. TOMASI T. B., Jr HUMAN GAMMA GLOBULIN. Blood. 1965 Mar;25:382–403. [PubMed] [Google Scholar]
  15. TOMASI T. B., Jr, TAN E. M., SOLOMON A., PRENDERGAST R. A. CHARACTERISTICS OF AN IMMUNE SYSTEM COMMON TO CERTAIN EXTERNAL SECRETIONS. J Exp Med. 1965 Jan 1;121:101–124. doi: 10.1084/jem.121.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. TOMASI T. B., Jr, ZIGELBAUM S. THE SELECTIVE OCCURENCE OF GAMMA-1A GLOBULINS IN CERTAIN BODY FLUIDS. J Clin Invest. 1963 Oct;42:1552–1560. doi: 10.1172/JCI104840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TURNER M. W., ROWE D. S. CHARACTERIZATION OF HUMAN ANTIBODIES TO SALMONELLA TYPHI BY GEL-FILTRATION AND ANTIGENIC ANALYSIS. Immunology. 1964 Nov;7:639–656. [PMC free article] [PubMed] [Google Scholar]
  18. Turner M. W., Rowe D. S. Antibodies of IgA and IgG class in normal human urine. Immunology. 1967 Jun;12(6):689–699. [PMC free article] [PubMed] [Google Scholar]
  19. Vaughan J. H., Jacox R. F., Gray B. A. Light and heavy chain components of gamma globulins in urines of normal persons and patients with agammaglobulinemia. J Clin Invest. 1967 Feb;46(2):266–279. doi: 10.1172/JCI105529. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES