Abstract
Most of the information concerning secretion changes in follicle-stimulating hormone (FSH) in humans has been gained with relatively insensitive bioassays of concentrates of pools of urine. We have developed a sensitive and specific radioimmunoassay for FSH that is 500-1000 times more sensitive than the rat ovarianweight augmentation assay and which is capable of quantifying FSH in small volumes of serum. Anti-FSH was prepared by immunizing rabbits with an impure FSH preparation. The majority of antisera showed complete inability to distinguish LH, TSH, and FSH, illustrating the immunological similarities of these hormones. One antiserum was specific when used in a radioimmunoassay. Potency estimates by bioassay were in good agreement, with a single exception, with those obtained with the radioimmunoassay for 10 FSH-containing preparations. Highly purified LH gave a higher potency by immunoassay than by bioassay.
Sera from eugonadal men contained 5-25 mIU/ml; sera from castrate men contained over 30 mIU/ml. Sera from eugonadal women contained 7-25 mIU/ml during the follicular phase and 5-15 mIU/ml during the luteal phase of the menstrual cycle. Sera from castrate or postmenopausal women contained 40-250 mIU/ml. FSH was measured throughout the menstrual cycle in 19 women. The general pattern that emerged is summarized as follows: there is a small early follicular phase rise in FSH, and then FSH is relatively constant until mid-cycle; in the majority of women a mid-cycle rise of FSH occurs coincidentally to the mid-cycle LH ovulatory peak; during the luteal phase FSH levels are relatively constant and lower than during the follicular phase. Nonsequential oral contraceptives containing estrogen and progestogen abolish these changes and FSH concentrations remain low throughout treatment. Treatment of castrate men and castrate or postmenopausal women with high doses of oral estrogens results in a fall of FSH to levels found in eugonadal men or women, but not to undetectable levels. Children less than 5 yr of age had undetectable FSH (< 5 mIU/ml).
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- BECKER K. L., ALBERT A. URINARY EXCRETION OF FOLLICLE-STIMULATING AND LUTEINIZING HORMONES. J Clin Endocrinol Metab. 1965 Jul;25:962–974. doi: 10.1210/jcem-25-7-962. [DOI] [PubMed] [Google Scholar]
- Bagshawe K. D., Wilde C. E., Orr A. H. Radioimmunoassay for human chorionic gonadotrophin and luteinising hormone. Lancet. 1966 May 21;1(7447):1118–1121. doi: 10.1016/s0140-6736(66)91136-6. [DOI] [PubMed] [Google Scholar]
- DENAMUR R., MAULEON P. [Endocrine control of persistence of the corpus luteum in sheep]. C R Hebd Seances Acad Sci. 1963 Jul 8;257:527–530. [PubMed] [Google Scholar]
- FUKUSHIMA M., STEVENS V. C., GANTT C. L., VORYS N. URINARY FSH AND LH EXCRETION DURING THE NORMAL MENSTRUAL CYCLE. J Clin Endocrinol Metab. 1964 Feb;24:205–213. doi: 10.1210/jcem-24-2-205. [DOI] [PubMed] [Google Scholar]
- GLICK S. M., ROTH J., LONERGAN E. T. SURVIVAL OF ENDOGENOUS HUMAN GROWTH HORMONE IN PLASMA. J Clin Endocrinol Metab. 1964 Jun;24:501–505. doi: 10.1210/jcem-24-6-501. [DOI] [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- IGARASHI M., MCCANN S. M. A NEW SENSITIVE BIO-ASSAY FOR FOLLICLE-STIMULATING HORMONES (FSH). Endocrinology. 1964 Mar;74:440–445. doi: 10.1210/endo-74-3-440. [DOI] [PubMed] [Google Scholar]
- Kohler P. O., Ross G. T., Odell W. D. Metabolic clearance and production rates of human luteinizing hormone in pre- and postmenopausal women. J Clin Invest. 1968 Jan;47(1):38–47. doi: 10.1172/JCI105713. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Malven P. V., Sawyer C. H. A luteolytic action of prolactin in hypophysectomized rats. Endocrinology. 1966 Aug;79(2):268–274. doi: 10.1210/endo-79-2-268. [DOI] [PubMed] [Google Scholar]
- McARTHUR J. W., WORCESTER J., INGERSOLL F. M. The urinary excretion of interstitial-cell and follicle-stimulating hormone activity during the normal menstrual cycle. J Clin Endocrinol Metab. 1958 Nov;18(11):1186–1201. doi: 10.1210/jcem-18-11-1186. [DOI] [PubMed] [Google Scholar]
- Meites J., Nicoll C. S. Adenohypophysis:prolactin. Annu Rev Physiol. 1966;28:57–88. doi: 10.1146/annurev.ph.28.030166.000421. [DOI] [PubMed] [Google Scholar]
- Midgley A. R., Jr, Jaffe R. B. Human luteinizing hormone in serum during the menstrual cycle: determination by radioimmunoassay. J Clin Endocrinol Metab. 1966 Dec;26(12):1375–1381. doi: 10.1210/jcem-26-12-1375. [DOI] [PubMed] [Google Scholar]
- Midgley A. R., Jr Radioimmunoassay: a method for human chorionic gonadotropin and human luteinizing hormone. Endocrinology. 1966 Jul;79(1):10–18. doi: 10.1210/endo-79-1-10. [DOI] [PubMed] [Google Scholar]
- Midgley A. R. Radioimmunoassay for human follicle-stimulating hormone. J Clin Endocrinol Metab. 1967 Feb;27(2):295–299. doi: 10.1210/jcem-27-2-295. [DOI] [PubMed] [Google Scholar]
- Odell W. D., Rayford P. L., Ross G. T. Simplified, partially automated method for radioimmunoassay of human thyroid-stimulating, growth, luteinizing, and follicle stimulating hormones. J Lab Clin Med. 1967 Dec;70(6):973–980. [PubMed] [Google Scholar]
- Odell W. D., Ross G. T., Rayford P. L. Radioimmunoassay for human luteinizing hormone. Metabolism. 1966 Apr;15(4):287–289. doi: 10.1016/0026-0495(66)90142-9. [DOI] [PubMed] [Google Scholar]
- Odell W. D., Ross G. T., Rayford P. L. Radioimmunoassay for luteinizing hormone in human plasma or serum: physiological studies. J Clin Invest. 1967 Feb;46(2):248–255. doi: 10.1172/JCI105527. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Odell W. D., Utiger R. D., Wilber J. F., Condliffe P. G. Estimation of the secretion rate of thyrotropin in man. J Clin Invest. 1967 Jun;46(6):953–959. doi: 10.1172/JCI105601. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Odell W. D., Wilber J. F., Paul W. E. Radioimmunoassay of thyrotropin in human serum. J Clin Endocrinol Metab. 1965 Sep;25(9):1179–1188. doi: 10.1210/jcem-25-9-1179. [DOI] [PubMed] [Google Scholar]
- Reichert L. E., Jr Further studies on the purification of human postmenopausal urinary luteinizing hormone. Endocrinology. 1967 Feb;80(2):319–323. doi: 10.1210/endo-80-2-319. [DOI] [PubMed] [Google Scholar]
- Rosemberg E., Keller P. J. Studies on the urinary excretion of follicle-stimulating and luteinizing hormone activity during the menstrual cycle. J Clin Endocrinol Metab. 1965 Sep;25(9):1262–1274. doi: 10.1210/jcem-25-9-1262. [DOI] [PubMed] [Google Scholar]
- Ross G. T., Odell W. D., Rayford P. L. Luteinizing hormone activity in plasma during the menstrual cycle. Science. 1967 Mar 31;155(3770):1679–1680. doi: 10.1126/science.155.3770.1679. [DOI] [PubMed] [Google Scholar]
- SATO T., GREENBLATT R. B., MAHESH V. B. LEVELS OF LUTEINIZING HORMONE DURING THE MENSTRUAL CYCLE DETERMINED BY IMMUNOLOGIC TECHNICS. Fertil Steril. 1965 Mar-Apr;16:223–228. doi: 10.1016/s0015-0282(16)35529-7. [DOI] [PubMed] [Google Scholar]
- SHORT R. V. OVARIAN STEROID SNNTHESIS AND SECRETION IN VIVO. Recent Prog Horm Res. 1964;20:303–340. [PubMed] [Google Scholar]
- STEELMAN S. L., POHLEY F. M. Assay of the follicle stimulating hormone based on the augmentation with human chorionic gonadotropin. Endocrinology. 1953 Dec;53(6):604–616. doi: 10.1210/endo-53-6-604. [DOI] [PubMed] [Google Scholar]
- STEVENS V. C., VORYS N., BESCH P. K., BARRY R. D. THE EFFECTS OF A NEW ORAL CONTRACEPTIVE ON GONADOTROPIN EXCRETION. Metabolism. 1965 Mar;14:SUPPL–SUPPL:338. doi: 10.1016/0026-0495(65)90020-x. [DOI] [PubMed] [Google Scholar]
- Saxena B. B., Demura H., Gandy H. M., Peterson R. E. Radioimmunoassay of human follicle stimulating and luteinizing hormones in plasma. J Clin Endocrinol Metab. 1968 Apr;28(4):519–534. doi: 10.1210/jcem-28-4-519. [DOI] [PubMed] [Google Scholar]
- WIDE L., GEMZELL C. Immunological determination of pituitary luteinizing hormone in the urine of fertile and post-menopausal women and adult men. Acta Endocrinol (Copenh) 1962 Apr;39:539–546. doi: 10.1530/acta.0.0390539. [DOI] [PubMed] [Google Scholar]