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. 1989 Aug;86(16):6338–6342. doi: 10.1073/pnas.86.16.6338

The beta-amyloid protein precursor of Alzheimer disease has soluble derivatives found in human brain and cerebrospinal fluid.

M R Palmert 1, M B Podlisny 1, D S Witker 1, T Oltersdorf 1, L H Younkin 1, D J Selkoe 1, S G Younkin 1
PMCID: PMC297834  PMID: 2503832

Abstract

In this study, we use antisera to synthetic beta-amyloid protein precursor (beta APP) peptides to identify, in human brain and cerebrospinal fluid (CSF), soluble approximately 125- and approximately 105-kDa derivatives of the beta APP that lack the carboxyl terminus of the full-length, membrane-associated forms. We show that the soluble approximately 125-kDa beta APP derivative contains the Kunitz protease inhibitor domain, whereas the approximately 105-kDa form does not, and we confirm that these two proteins are soluble beta APP derivatives by purifying each from human CSF and directly sequencing its amino terminus.

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