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. 1989 Dec;86(23):9144–9148. doi: 10.1073/pnas.86.23.9144

Isolation and characterization of s-myc, a member of the rat myc gene family.

A Sugiyama 1, A Kume 1, K Nemoto 1, S Y Lee 1, Y Asami 1, F Nemoto 1, S Nishimura 1, Y Kuchino 1
PMCID: PMC298450  PMID: 2594755

Abstract

A 9-kilobase-pair clone containing a myc-related gene that we have designated s-myc was isolated from a rat genomic library. The entire nucleotide sequence of the cloned DNA was determined, showing that the s-myc gene contains an open reading frame consisting of 1287 base pairs without introns. In vitro transcription-translation analysis of s-myc indicated that this gene produces a protein of approximately 50 kDa. The amino acid sequence predicted from the DNA sequence showed that the s-Myc protein is closely related to the murine N-Myc protein but lacks an acidic amino acid-rich sequence commonly present in the Myc-family proteins. Studies on transfection of s-myc into rat RT4-AC tumor cells revealed that the gene produces a polyadenylylated transcript of approximately 4.7 kilobases and that its high-level expression suppresses the tumor-igenicity of RT4-AC tumor cells in nude mice.

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Selected References

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