Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1987 Aug;84(15):5267–5271. doi: 10.1073/pnas.84.15.5267

Interleukin 1 regulates hematopoietic activity, a role previously ascribed to hemopoietin 1.

D Y Mochizuki, J R Eisenman, P J Conlon, A D Larsen, R J Tushinski
PMCID: PMC298836  PMID: 2885846

Abstract

A murine in vitro assay was developed to measure potentiation of a proliferative response to suboptimal concentrations of the hematopoietic regulatory molecule granulocyte/macrophage colony-stimulating factor by an immature bone marrow population. The assay, designated the 5-fluorouracil bone marrow proliferation assay, was used to characterize potentiating activity in serum-free culture supernatants of the human tumor cell line HBT 5637. Molecular and biochemical analyses indicated that the HBT 5637-derived potentiating activity could be attributed to interleukin 1 alpha. Serologic analysis using a monoclonal antibody against purified recombinant interleukin 1 alpha proved conclusively that the potentiating activity in HBT 5637 serum-free supernatants is due to interleukin 1 alpha. From these data, the activity of interleukin 1 alpha seems to be the same synergistic activity formerly ascribed to hemopoietin 1.

Full text

PDF
5268

Images in this article

5268Image
on p.5268

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartelmez S. H., Stanley E. R. Synergism between hemopoietic growth factors (HGFs) detected by their effects on cells bearing receptors for a lineage specific HGF: assay of hemopoietin-1. J Cell Physiol. 1985 Mar;122(3):370–378. doi: 10.1002/jcp.1041220306. [DOI] [PubMed] [Google Scholar]
  2. Bradley T. R., Hodgson G. S. Detection of primitive macrophage progenitor cells in mouse bone marrow. Blood. 1979 Dec;54(6):1446–1450. [PubMed] [Google Scholar]
  3. Bradley T. R., Metcalf D. The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci. 1966 Jun;44(3):287–299. doi: 10.1038/icb.1966.28. [DOI] [PubMed] [Google Scholar]
  4. Cantrell M. A., Anderson D., Cerretti D. P., Price V., McKereghan K., Tushinski R. J., Mochizuki D. Y., Larsen A., Grabstein K., Gillis S. Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6250–6254. doi: 10.1073/pnas.82.18.6250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Conlon P. J. A rapid biologic assay for the detection of interleukin 1. J Immunol. 1983 Sep;131(3):1280–1282. [PubMed] [Google Scholar]
  6. Conlon P. J., Luk K. H., Park L. S., March C. J., Hopp T. P., Urdal D. L. Generation of anti-peptide monoclonal antibodies which recognize mature CSF-2 alpha (IL 3) protein. J Immunol. 1985 Jul;135(1):328–332. [PubMed] [Google Scholar]
  7. Dinarello C. A. Interleukin-1. Rev Infect Dis. 1984 Jan-Feb;6(1):51–95. doi: 10.1093/clinids/6.1.51. [DOI] [PubMed] [Google Scholar]
  8. Dower S. K., Kronheim S. R., Hopp T. P., Cantrell M., Deeley M., Gillis S., Henney C. S., Urdal D. L. The cell surface receptors for interleukin-1 alpha and interleukin-1 beta are identical. Nature. 1986 Nov 20;324(6094):266–268. doi: 10.1038/324266a0. [DOI] [PubMed] [Google Scholar]
  9. Durum S. K., Schmidt J. A., Oppenheim J. J. Interleukin 1: an immunological perspective. Annu Rev Immunol. 1985;3:263–287. doi: 10.1146/annurev.iy.03.040185.001403. [DOI] [PubMed] [Google Scholar]
  10. Gabrilove J. L., Welte K., Harris P., Platzer E., Lu L., Levi E., Mertelsmann R., Moore M. A. Pluripoietin alpha: a second human hematopoietic colony-stimulating factor produced by the human bladder carcinoma cell line 5637. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2478–2482. doi: 10.1073/pnas.83.8.2478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gough N. M., Gough J., Metcalf D., Kelso A., Grail D., Nicola N. A., Burgess A. W., Dunn A. R. Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor. 1984 Jun 28-Jul 4Nature. 309(5971):763–767. doi: 10.1038/309763a0. [DOI] [PubMed] [Google Scholar]
  12. Grabstein K., Mochizuki D., Kronheim S., Price V., Cosman D., Urdal D., Gillis S., Conlon P. Regulation of antibody production in vitro by granulocyte-macrophage colony stimulating factor. J Mol Cell Immunol. 1986;2(4):199–207. [PubMed] [Google Scholar]
  13. Jubinsky P. T., Stanley E. R. Purification of hemopoietin 1: a multilineage hemopoietic growth factor. Proc Natl Acad Sci U S A. 1985 May;82(9):2764–2768. doi: 10.1073/pnas.82.9.2764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kaye J., Gillis S., Mizel S. B., Shevach E. M., Malek T. R., Dinarello C. A., Lachman L. B., Janeway C. A., Jr Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J Immunol. 1984 Sep;133(3):1339–1345. [PubMed] [Google Scholar]
  15. March C. J., Mosley B., Larsen A., Cerretti D. P., Braedt G., Price V., Gillis S., Henney C. S., Kronheim S. R., Grabstein K. Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature. 1985 Jun 20;315(6021):641–647. doi: 10.1038/315641a0. [DOI] [PubMed] [Google Scholar]
  16. Mochizuki D. Y., Eisenman J. R., Conlon P. J., Park L. S., Urdal D. L. Development and characterization of antiserum to murine granulocyte-macrophage colony-stimulating factor. J Immunol. 1986 May 15;136(10):3706–3709. [PubMed] [Google Scholar]
  17. Männel D. N., Mizel S. B., Diamantstein T., Falk W. Induction of interleukin 2 responsiveness in thymocytes by synergistic action of interleukin 1 and interleukin 2. J Immunol. 1985 May;134(5):3108–3110. [PubMed] [Google Scholar]
  18. Nicola N. A. Granulocyte colony stimulating factor. Methods Enzymol. 1985;116:600–619. doi: 10.1016/s0076-6879(85)16046-5. [DOI] [PubMed] [Google Scholar]
  19. Park L. S., Friend D., Gillis S., Urdal D. L. Characterization of the cell surface receptor for granulocyte-macrophage colony-stimulating factor. J Biol Chem. 1986 Mar 25;261(9):4177–4183. [PubMed] [Google Scholar]
  20. Prestidge R. L., Hearn M. T. Preparative flatbed electrofocusing in granulated gels with natural pH gradients generated from simple buffers. Anal Biochem. 1979 Aug;97(1):95–102. doi: 10.1016/0003-2697(79)90332-4. [DOI] [PubMed] [Google Scholar]
  21. Sieff C. A., Emerson S. G., Donahue R. E., Nathan D. G., Wang E. A., Wong G. G., Clark S. C. Human recombinant granulocyte-macrophage colony-stimulating factor: a multilineage hematopoietin. Science. 1985 Dec 6;230(4730):1171–1173. doi: 10.1126/science.3877981. [DOI] [PubMed] [Google Scholar]
  22. Souza L. M., Boone T. C., Gabrilove J., Lai P. H., Zsebo K. M., Murdock D. C., Chazin V. R., Bruszewski J., Lu H., Chen K. K. Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells. Science. 1986 Apr 4;232(4746):61–65. doi: 10.1126/science.2420009. [DOI] [PubMed] [Google Scholar]
  23. Stanley E. R., Bartocci A., Patinkin D., Rosendaal M., Bradley T. R. Regulation of very primitive, multipotent, hemopoietic cells by hemopoietin-1. Cell. 1986 Jun 6;45(5):667–674. doi: 10.1016/0092-8674(86)90781-6. [DOI] [PubMed] [Google Scholar]
  24. Stanley E. R., Heard P. M. Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells. J Biol Chem. 1977 Jun 25;252(12):4305–4312. [PubMed] [Google Scholar]
  25. Tushinski R. J., Oliver I. T., Guilbert L. J., Tynan P. W., Warner J. R., Stanley E. R. Survival of mononuclear phagocytes depends on a lineage-specific growth factor that the differentiated cells selectively destroy. Cell. 1982 Jan;28(1):71–81. doi: 10.1016/0092-8674(82)90376-2. [DOI] [PubMed] [Google Scholar]
  26. Urdal D. L., Mochizuki D., Conlon P. J., March C. J., Remerowski M. L., Eisenman J., Ramthun C., Gillis S. Lymphokine purification by reversed-phase high-performance liquid chromatography. J Chromatogr. 1984 Jul 27;296:171–179. doi: 10.1016/s0021-9673(01)96410-6. [DOI] [PubMed] [Google Scholar]
  27. Watson J. D., Crosier P. S., March C. J., Conlon P. J., Mochizuki D. Y., Gillis S., Urdal D. L. Purification to homogeneity of a human hematopoietic growth factor that stimulates the growth of a murine interleukin 3-dependent cell line. J Immunol. 1986 Aug 1;137(3):854–857. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES