Abstract
The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.
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- Adari H. Y., Rose K., Williams K. R., Konigsberg W. H., Lin T. C., Spicer E. K. Cloning, nucleotide sequence, and overexpression of the bacteriophage T4 regA gene. Proc Natl Acad Sci U S A. 1985 Apr;82(7):1901–1905. doi: 10.1073/pnas.82.7.1901. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Alberts B. M. The DNA enzymology of protein machines. Cold Spring Harb Symp Quant Biol. 1984;49:1–12. doi: 10.1101/sqb.1984.049.01.003. [DOI] [PubMed] [Google Scholar]
- Amann E., Brosius J., Ptashne M. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene. 1983 Nov;25(2-3):167–178. doi: 10.1016/0378-1119(83)90222-6. [DOI] [PubMed] [Google Scholar]
- Bikel I., Roberts T. M., Bladon M. T., Green R., Amann E., Livingston D. M. Purification of biologically active simian virus 40 small tumor antigen. Proc Natl Acad Sci U S A. 1983 Feb;80(4):906–910. doi: 10.1073/pnas.80.4.906. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brutlag D., Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. J Biol Chem. 1972 Jan 10;247(1):241–248. [PubMed] [Google Scholar]
- Burgess R. R., Jendrisak J. J. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry. 1975 Oct 21;14(21):4634–4638. doi: 10.1021/bi00692a011. [DOI] [PubMed] [Google Scholar]
- Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem. 1981 Apr;112(2):295–298. doi: 10.1016/0003-2697(81)90296-7. [DOI] [PubMed] [Google Scholar]
- Formosa T., Burke R. L., Alberts B. M. Affinity purification of bacteriophage T4 proteins essential for DNA replication and genetic recombination. Proc Natl Acad Sci U S A. 1983 May;80(9):2442–2446. doi: 10.1073/pnas.80.9.2442. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gribskov M., Burgess R. R. Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase. Gene. 1983 Dec;26(2-3):109–118. doi: 10.1016/0378-1119(83)90180-4. [DOI] [PubMed] [Google Scholar]
- Hibner U., Alberts B. M. Fidelity of DNA replication catalysed in vitro on a natural DNA template by the T4 bacteriophage multi-enzyme complex. Nature. 1980 May 29;285(5763):300–305. doi: 10.1038/285300a0. [DOI] [PubMed] [Google Scholar]
- Hughes M. B., Yee A. M., Dawson M., Karam J. Genetic mapping of the amino-terminal domain of bacteriophage T4 DNA polymerase. Genetics. 1987 Mar;115(3):393–403. doi: 10.1093/genetics/115.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Mace D. C., Alberts B. M. Characterization of the stimulatory effect of T4 gene 45 protein and the gene 44/62 protein complex on DNA synthesis by T4 DNA polymerase. J Mol Biol. 1984 Aug 5;177(2):313–327. doi: 10.1016/0022-2836(84)90459-5. [DOI] [PubMed] [Google Scholar]
- Miller R. C., Jr, Young E. T., 2nd, Epstein R. H., Krisch H. M., Mattson T., Bolle A. Regulation of the synthesis of the T4 DNA polymerase (gene 43). Virology. 1981 Apr 15;110(1):98–112. doi: 10.1016/0042-6822(81)90011-8. [DOI] [PubMed] [Google Scholar]
- Nossal N. G., Peterlin B. M. DNA replication by bacteriophage T4 proteins. The T4 43, 32, 44--62, And 45 proteins are required for strand displacement synthesis at nicks in duplex DNA. J Biol Chem. 1979 Jul 10;254(13):6032–6037. [PubMed] [Google Scholar]
- Nossal N. G. Prokaryotic DNA replication systems. Annu Rev Biochem. 1983;52:581–615. doi: 10.1146/annurev.bi.52.070183.003053. [DOI] [PubMed] [Google Scholar]
- Russel M. Control of bacteriophage T4 DNA polymerase synthesis. J Mol Biol. 1973 Sep 5;79(1):83–94. doi: 10.1016/0022-2836(73)90271-4. [DOI] [PubMed] [Google Scholar]
- Scheuermann R. H., Echols H. A separate editing exonuclease for DNA replication: the epsilon subunit of Escherichia coli DNA polymerase III holoenzyme. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7747–7751. doi: 10.1073/pnas.81.24.7747. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shamoo Y., Adari H., Konigsberg W. H., Williams K. R., Chase J. W. Cloning of T4 gene 32 and expression of the wild-type protein under lambda promoter PL regulation in Escherichia coli. Proc Natl Acad Sci U S A. 1986 Dec;83(23):8844–8848. doi: 10.1073/pnas.83.23.8844. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simons G., Remaut E., Allet B., Devos R., Fiers W. High-level expression of human interferon gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda. Gene. 1984 Apr;28(1):55–64. doi: 10.1016/0378-1119(84)90087-8. [DOI] [PubMed] [Google Scholar]
- Sinha N. K., Morris C. F., Alberts B. M. Efficient in vitro replication of double-stranded DNA templates by a purified T4 bacteriophage replication system. J Biol Chem. 1980 May 10;255(9):4290–4293. [PubMed] [Google Scholar]
- Wilson G. G., Murray N. E. Molecular cloning of the DNA ligase gene from bacteriophage T4. I. Characterisation of the recombinants. J Mol Biol. 1979 Aug 15;132(3):471–491. doi: 10.1016/0022-2836(79)90270-5. [DOI] [PubMed] [Google Scholar]
- Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
- de Boer H. A., Comstock L. J., Vasser M. The tac promoter: a functional hybrid derived from the trp and lac promoters. Proc Natl Acad Sci U S A. 1983 Jan;80(1):21–25. doi: 10.1073/pnas.80.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]