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. 1987 Oct;84(20):7000–7004. doi: 10.1073/pnas.84.20.7000

Cloning and expression of T4 DNA polymerase.

T C Lin 1, J Rush 1, E K Spicer 1, W H Konigsberg 1
PMCID: PMC299216  PMID: 3478676

Abstract

The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.

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Selected References

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