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. 1987 Oct;84(20):7139–7143. doi: 10.1073/pnas.84.20.7139

Primary structure and subcellular localization of the knob-associated histidine-rich protein of Plasmodium falciparum.

L G Pologe 1, A Pavlovec 1, H Shio 1, J V Ravetch 1
PMCID: PMC299245  PMID: 3313387

Abstract

Plasmodium falciparum-infected erythrocytes bind to venular endothelial cells by means of electron-dense deformations (knobs) on the parasitized erythrocyte surface. The primary structure of a parasite-derived histidine-rich protein associated with the knob structure was deduced from cDNA sequence analysis. The 634 amino acid sequence is rich in lysine and histidine and contains three distinct, tandemly repeated domains. Indirect immunofluorescence, using affinity-purified monospecific antibodies directed against recombinant protein synthesized in Escherichia coli, localized the knob-associated histidine-rich protein to the membrane of knobby infected erythrocytes. Immunoelectron microscopy established that the protein is clustered on the cytoplasmic side of the erythrocyte membrane and is associated with the electron-dense knobs. A role for this histidine-rich protein in knob structure and cytoadherence is suggested based upon these data.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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