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. 1987 Nov;84(21):7778–7782. doi: 10.1073/pnas.84.21.7778

Electrophysiological and autoradiographical evidence of V1 vasopressin receptors in the lateral septum of the rat brain.

M Raggenbass 1, E Tribollet 1, J J Dreifuss 1
PMCID: PMC299384  PMID: 2959962

Abstract

Extracellular recordings were obtained from single neurons located in the lateral septum, an area known to receive a vasopressinergic innervation in the rat brain. Approximately half of the neurons tested responded to 8-L-arginine vasopressin (AVP) by a marked increase in firing rate at concentrations greater than 1 nM. The effect of vasopressin was blocked by synthetic structural analogues possessing antagonistic properties on peripheral vasopressin and oxytocin receptors. Oxytocin was much less potent than vasopressin in firing septal neurons, and a selective oxytocic agonist was totally ineffective. The action of vasopressin on neuronal firing was mimicked by the vasopressor agonist [2-phenylalanine,8-ornithine]vasotocin but not by the selective antidiuretic agonist 1-deamino[8-D-arginine]vasopressin. In a parallel study, sites that bind [3H]AVP at low concentration (1.5 nM) were found by in vitro autoradiography in the lateral septum. Adjacent sections were also incubated with 1.5 mM [3H]AVP and, in addition, with 100 nM [2-phenylalanine,8-ornithine]vasotocin or 1-deamino[8-D-arginine]vasopressin--i.e., the same compounds as those used for the electrophysiological study. Results showed that the vasopressor agonist, but not the antidiuretic agonist, displaced [3H]AVP, thus indicating that the vasopressin binding sites detected by autoradiography in the septum were V1 (vasopressor type) rather than V2 (antidiuretic type) receptors. Based on the electrophysiological evidence, we conclude that these receptors, when occupied, lead to increased firing of lateral septal neurons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Audigier S., Barberis C. Pharmacological characterization of two specific binding sites for neurohypophyseal hormones in hippocampal synaptic plasma membranes of the rat. EMBO J. 1985 Jun;4(6):1407–1412. doi: 10.1002/j.1460-2075.1985.tb03794.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baskin D. G., Petracca F., Dorsa D. M. Autoradiographic localization of specific binding sites for [3H][Arg8]vasopressin in the septum of the rat brain with tritium-sensitive film. Eur J Pharmacol. 1983 May 20;90(1):155–157. doi: 10.1016/0014-2999(83)90231-5. [DOI] [PubMed] [Google Scholar]
  3. Berde B., Boissonnas R. A., Huguenin R. L., Stürmer E. Vasopressin analogues with selective pressor activity. Experientia. 1964 Jan 15;20(1):42–43. doi: 10.1007/BF02146034. [DOI] [PubMed] [Google Scholar]
  4. Biegon A., Terlou M., Voorhuis T. D., de Kloet E. R. Arginine-vasopressin binding sites in rat brain: a quantitative autoradiographic study. Neurosci Lett. 1984 Feb 24;44(3):229–234. doi: 10.1016/0304-3940(84)90027-2. [DOI] [PubMed] [Google Scholar]
  5. Buijs R. M., Swaab D. F. Immuno-electron microscopical demonstration of vasopressin and oxytocin synapses in the limbic system of the rat. Cell Tissue Res. 1979;204(3):355–365. doi: 10.1007/BF00233648. [DOI] [PubMed] [Google Scholar]
  6. Buijs R. M., Van Heerikhuize J. J. Vasopressin and oxytocin release in the brain--a synaptic event. Brain Res. 1982 Dec 2;252(1):71–76. doi: 10.1016/0006-8993(82)90979-9. [DOI] [PubMed] [Google Scholar]
  7. Cornett L. E., Dorsa D. M. Vasopressin receptor subtypes in dorsal hindbrain and renal medulla. Peptides. 1985 Jan-Feb;6(1):85–89. doi: 10.1016/0196-9781(85)90081-6. [DOI] [PubMed] [Google Scholar]
  8. De Kloet E. R., Rotteveel F., Voorhuis T. A., Terlou M. Topography of binding sites for neurohypophyseal hormones in rat brain. Eur J Pharmacol. 1985 Mar 26;110(1):113–119. doi: 10.1016/0014-2999(85)90036-6. [DOI] [PubMed] [Google Scholar]
  9. De Vries G. J., Buijs R. M. The origin of the vasopressinergic and oxytocinergic innervation of the rat brain with special reference to the lateral septum. Brain Res. 1983 Aug 29;273(2):307–317. doi: 10.1016/0006-8993(83)90855-7. [DOI] [PubMed] [Google Scholar]
  10. DeVries G. J., Buijs R. M., Van Leeuwen F. W., Caffé A. R., Swaab D. F. The vasopressinergic innervation of the brain in normal and castrated rats. J Comp Neurol. 1985 Mar 8;233(2):236–254. doi: 10.1002/cne.902330206. [DOI] [PubMed] [Google Scholar]
  11. Demotes-Mainard J., Chauveau J., Rodriguez F., Vincent J. D., Poulain D. A. Septal release of vasopressin in response to osmotic, hypovolemic and electrical stimulation in rats. Brain Res. 1986 Sep 3;381(2):314–321. doi: 10.1016/0006-8993(86)90082-x. [DOI] [PubMed] [Google Scholar]
  12. Dorsa D. M., Petracca F. M., Baskin D. G., Cornett L. E. Localization and characterization of vasopressin-binding sites in the amygdala of the rat brain. J Neurosci. 1984 Jul;4(7):1764–1770. doi: 10.1523/JNEUROSCI.04-07-01764.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hoorneman E. M., Buijs R. M. Vasopressin fiber pathways in the rat brain following suprachiasmatic nucleus lesioning. Brain Res. 1982 Jul 15;243(2):235–241. doi: 10.1016/0006-8993(82)90246-3. [DOI] [PubMed] [Google Scholar]
  14. Huwyler T., Felix D. Angiotensin II-sensitive neurons in septal areas of the rat. Brain Res. 1980 Aug 11;195(1):187–195. doi: 10.1016/0006-8993(80)90876-8. [DOI] [PubMed] [Google Scholar]
  15. Joëls M., Urban I. J. Arginine-vasopressin enhances the responses of lateral septal neurons in the rat to excitatory amino acids and fimbria-fornix stimuli. Brain Res. 1984 Oct 8;311(2):201–209. doi: 10.1016/0006-8993(84)90084-2. [DOI] [PubMed] [Google Scholar]
  16. Joëls M., Urban I. J. The effect of microiontophoretically applied vasopressin and oxytocin on single neurones in the septum and dorsal hippocampus of the rat. Neurosci Lett. 1982 Nov 16;33(1):79–84. doi: 10.1016/0304-3940(82)90133-1. [DOI] [PubMed] [Google Scholar]
  17. Kruszynski M., Lammek B., Manning M., Seto J., Haldar J., Sawyer W. H. [1-beta-Mercapto-beta,beta-cyclopentamethylenepropionic acid),2-(O-methyl)tyrosine ]argine-vasopressin and [1-beta-mercapto-beta,beta-cyclopentamethylenepropionic acid)]argine-vasopressine, two highly potent antagonists of the vasopressor response to arginine-vasopressin. J Med Chem. 1980 Apr;23(4):364–368. doi: 10.1021/jm00178a003. [DOI] [PubMed] [Google Scholar]
  18. Lowbridge J., Manning M., Haldar J., Sawyer W. H. Synthesis and some pharmacological properties of [4-threonine, 7-glycine]oxytocin, [1-(L-2-hydroxy-3-mercaptopropanoic acid), 4-threonine, 7-glycine]oxytocin (hydroxy[Thr4, Gly7]oxytocin), and [7-Glycine]oxytocin, peptides with high oxytocic-antidiuretic selectivity. J Med Chem. 1977 Jan;20(1):120–123. doi: 10.1021/jm00211a025. [DOI] [PubMed] [Google Scholar]
  19. Manning M., Balaspiri L., Moehring J., Haldar J., Sawyer W. H. Synthesis and some pharmacological properties of deamino(4-threonine,8-D-arginine)vasopressin and deamino(8-D-arginine)vasopressin, highly potent and specific antidiuretic peptides, and (8-D-arginine)vasopressin and deamino-arginine-vasopressin. J Med Chem. 1976 Jun;19(6):842–845. doi: 10.1021/jm00228a023. [DOI] [PubMed] [Google Scholar]
  20. Manning M., Lammek B., Bankowski K., Seto J., Sawyer W. H. Synthesis and some pharmacological properties of 18 potent O-alkyltyrosine-substituted antagonists of the vasopressor responses to arginine-vasopressin. J Med Chem. 1985 Oct;28(10):1485–1491. doi: 10.1021/jm00148a019. [DOI] [PubMed] [Google Scholar]
  21. Marchand J. E., Hagino N. Effect of iontophoresis of vasopressin on lateral septal neurons. Exp Neurol. 1982 Dec;78(3):790–795. doi: 10.1016/0014-4886(82)90093-0. [DOI] [PubMed] [Google Scholar]
  22. Michell R. H., Kirk C. J., Billah M. M. Hormonal stimulation of phosphatidylinositol breakdown with particular reference to the hepatic effects of vasopressin. Biochem Soc Trans. 1979 Oct;7(5):861–865. doi: 10.1042/bst0070861. [DOI] [PubMed] [Google Scholar]
  23. Poulain D. A., Wakerley J. B. Electrophysiology of hypothalamic magnocellular neurones secreting oxytocin and vasopressin. Neuroscience. 1982 Apr;7(4):773–808. doi: 10.1016/0306-4522(82)90044-6. [DOI] [PubMed] [Google Scholar]
  24. Urban I. J., De Wied D. Effect of vasopressin, oxytocin and peptides derived from these hormones on field potential induced in lateral septum of rats by stimulation of the fimbria fornix. Neuropeptides. 1986 Jan;7(1):41–49. doi: 10.1016/0143-4179(86)90078-8. [DOI] [PubMed] [Google Scholar]
  25. Van Leeuwen F. W., Wolters P. Light microscopic autoradiographic localization of [3H]arginine-vasopressin binding sites in the rat brain and kidney. Neurosci Lett. 1983 Oct 31;41(1-2):61–66. doi: 10.1016/0304-3940(83)90223-9. [DOI] [PubMed] [Google Scholar]

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