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. 1987 Dec;84(23):8370–8374. doi: 10.1073/pnas.84.23.8370

cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase.

B W Cherney 1, O W McBride 1, D F Chen 1, H Alkhatib 1, K Bhatia 1, P Hensley 1, M E Smulson 1
PMCID: PMC299544  PMID: 2891139

Abstract

Recently we described a full-length cDNA for the human nuclear enzyme poly(ADP-ribose) polymerase. Here, we report the chromosomal localization and partial map of the human gene for this enzyme as well as the complete coding sequence for this protein. The nucleotide sequence reveals a single 3042-base open reading frame encoding a protein with a predicted Mr of 113,135. A comparison of this deduced amino acid sequence with the amino acid sequence of three peptides derived from human poly(ADP-ribose) polymerase revealed a match of 27 amino acid residues. A computer-derived structural analysis of the enzyme and a search for similarities with other proteins confirmed that the polymerase belongs to a subfamily of DNA/NAD-binding proteins and DNA-repair proteins. Possible Zn2+-binding "fingers," a nucleotide-binding fold, and a nuclear transport signal were noted. Additionally, chromosomal mapping has identified polymerase-hybridizing sequences on human chromosomes 1 (the active gene), 13, and 14 (processed pseudogenes). Using the polymerase cDNA as a probe, we also have detected several DNA restriction fragment length polymorphisms in normal humans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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