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. 1987 Dec;84(23):8530–8534. doi: 10.1073/pnas.84.23.8530

Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium.

R L Charlebois 1, W L Lam 1, S W Cline 1, W F Doolittle 1
PMCID: PMC299578  PMID: 2825193

Abstract

We determined the complete nucleotide sequence of the 6354-base-pair plasmid pHV2 of the archaebacterium Halobacterium volcanii. This plasmid is present in approximately six copies per chromosome. We have generated a strain, H. volcanii WFD11, cured of pHV2 by treatment of liquid cultures with ethidium bromide. We describe PEG-mediated transformation of H. volcanii WFD11 with intact pHV2 and with a form of pHV2 marked by a 93-base-pair deletion generated in vitro.

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Selected References

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