Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1987 Dec;84(24):9030–9034. doi: 10.1073/pnas.84.24.9030

Differential expression of Na+,K+-ATPase alpha- and beta-subunit mRNAs in rat tissues and cell lines.

J R Emanuel 1, S Garetz 1, L Stone 1, R Levenson 1
PMCID: PMC299685  PMID: 2827165

Abstract

We have analyzed Na+,K+-ATPase (EC 3.6.1.3) alpha- and beta-subunit mRNA expression in rat tissues and cell lines derived from the rat central nervous system. Substantial differences in the tissue and developmental specificity of expression were found for the genes encoding three isoforms of the alpha subunit. Transcripts of the alpha 1-subunit gene were detected in all tissues tested, whereas alpha 2- and alpha 3-subunit mRNA species were expressed predominantly in brain. The pattern of expression of beta-subunit mRNA also was complex and tissue specific but was distinct from that of any of the alpha-subunit mRNAs. Cell lines derived from the rat central nervous system and the pheochromocytoma PC12 expressed the mRNAs for all three alpha-subunit isoforms, whereas beta-subunit mRNA was detected only in PC12 cells. The distinct expression patterns of rat Na+,K+-ATPase mRNAs suggest that different members of the ATPase family may have specialized functions.

Full text

PDF
9031

Images in this article

9032Image
on p.9032
9032Image
on p.9032
9032Image
on p.9032
9032Image
on p.9032
9032Image
on p.9032
9032Image
on p.9032

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  2. Emanuel J. R., Garetz S., Schneider J., Ash J. F., Benz E. J., Jr, Levenson R. Amplification of DNA sequences coding for the Na,K-ATPase alpha-subunit in ouabain-resistant C+ cells. Mol Cell Biol. 1986 Jul;6(7):2476–2481. doi: 10.1128/mcb.6.7.2476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  4. Fisher J. A., Baxter-Lowe L. A., Hokin L. E. Regulation of Na,K-ATPase biosynthesis in developing Artemia salina. J Biol Chem. 1986 Jan 15;261(2):515–519. [PubMed] [Google Scholar]
  5. Geering K., Girardet M., Bron C., Kraehenbühl J. P., Rossier B. C. Hormonal regulation of (Na+,K+)-ATPase biosynthesis in the toad bladder. Effect of aldosterone and 3,5,3'-triiodo-L-thyronine. J Biol Chem. 1982 Sep 10;257(17):10338–10343. [PubMed] [Google Scholar]
  6. Goldberg D. A. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794–5798. doi: 10.1073/pnas.77.10.5794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hubert J. J., Schenk D. B., Skelly H., Leffert H. L. Rat hepatic (Na+, K+)-ATPase: alpha-subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping. Biochemistry. 1986 Jul 15;25(14):4156–4163. doi: 10.1021/bi00362a025. [DOI] [PubMed] [Google Scholar]
  8. Kent R. B., Emanuel J. R., Ben Neriah Y., Levenson R., Housman D. E. Ouabain resistance conferred by expression of the cDNA for a murine Na+, K+-ATPase alpha subunit. Science. 1987 Aug 21;237(4817):901–903. doi: 10.1126/science.3039660. [DOI] [PubMed] [Google Scholar]
  9. Kent R. B., Fallows D. A., Geissler E., Glaser T., Emanuel J. R., Lalley P. A., Levenson R., Housman D. E. Genes encoding alpha and beta subunits of Na,K-ATPase are located on three different chromosomes in the mouse. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5369–5373. doi: 10.1073/pnas.84.15.5369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lo C. S., Edelman I. S. Effect of triiodothyronine on the synthesis and degradation of renal cortical (Na+ + k+)-adenosine triphosphatase. J Biol Chem. 1976 Dec 25;251(24):7834–7840. [PubMed] [Google Scholar]
  11. Lytton J., Lin J. C., Guidotti G. Identification of two molecular forms of (Na+,K+)-ATPase in rat adipocytes. Relation to insulin stimulation of the enzyme. J Biol Chem. 1985 Jan 25;260(2):1177–1184. [PubMed] [Google Scholar]
  12. McGrail K. M., Sweadner K. J. Immunofluorescent localization of two different Na,K-ATPases in the rat retina and in identified dissociated retinal cells. J Neurosci. 1986 May;6(5):1272–1283. doi: 10.1523/JNEUROSCI.06-05-01272.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mercer R. W., Schneider J. W., Savitz A., Emanuel J., Benz E. J., Jr, Levenson R. Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells. Mol Cell Biol. 1986 Nov;6(11):3884–3890. doi: 10.1128/mcb.6.11.3884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schellenberg G. D., Pech I. V., Stahl W. L. Immunoreactivity of subunits of the (Na+ + K+)-ATPase. Cross-reactivity of the alpha, alpha + and beta forms in different organs and species. Biochim Biophys Acta. 1981 Dec 21;649(3):691–700. doi: 10.1016/0005-2736(81)90173-5. [DOI] [PubMed] [Google Scholar]
  15. Schmitt C. A., McDonough A. A. Developmental and thyroid hormone regulation of two molecular forms of Na+-K+-ATPase in brain. J Biol Chem. 1986 Aug 5;261(22):10439–10444. [PubMed] [Google Scholar]
  16. Schneider J. W., Mercer R. W., Caplan M., Emanuel J. R., Sweadner K. J., Benz E. J., Jr, Levenson R. Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6357–6361. doi: 10.1073/pnas.82.18.6357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schubert D., Heinemann S., Carlisle W., Tarikas H., Kimes B., Patrick J., Steinbach J. H., Culp W., Brandt B. L. Clonal cell lines from the rat central nervous system. Nature. 1974 May 17;249(454):224–227. doi: 10.1038/249224a0. [DOI] [PubMed] [Google Scholar]
  18. Shull G. E., Greeb J., Lingrel J. B. Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain. Biochemistry. 1986 Dec 16;25(25):8125–8132. doi: 10.1021/bi00373a001. [DOI] [PubMed] [Google Scholar]
  19. Shull M. M., Lingrel J. B. Multiple genes encode the human Na+,K+-ATPase catalytic subunit. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4039–4043. doi: 10.1073/pnas.84.12.4039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Siegel G. J., Desmond T., Ernst S. A. Immunoreactivity and ouabain-dependent phosphorylation of (Na+ + K+)-adenosinetriphosphatase catalytic subunit doublets. J Biol Chem. 1986 Oct 15;261(29):13768–13776. [PubMed] [Google Scholar]
  21. Specht S. C., Sweadner K. J. Two different Na,K-ATPases in the optic nerve: cells of origin and axonal transport. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1234–1238. doi: 10.1073/pnas.81.4.1234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sweadner K. J. Two molecular forms of (Na+ + K+)-stimulated ATPase in brain. Separation, and difference in affinity for strophanthidin. J Biol Chem. 1979 Jul 10;254(13):6060–6067. [PubMed] [Google Scholar]
  23. Tamkun M. M., Fambrough D. M. The (Na+ + K+)-ATPase of chick sensory neurons. Studies on biosynthesis and intracellular transport. J Biol Chem. 1986 Jan 25;261(3):1009–1019. [PubMed] [Google Scholar]
  24. Thomas R. C. Electrogenic sodium pump in nerve and muscle cells. Physiol Rev. 1972 Jul;52(3):563–594. doi: 10.1152/physrev.1972.52.3.563. [DOI] [PubMed] [Google Scholar]
  25. Urayama O., Nakao M. Organ secificity of rat sodium- and potassium-activated adenosine triphosphatase. J Biochem. 1979 Nov;86(5):1371–1381. doi: 10.1093/oxfordjournals.jbchem.a132654. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES