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. 1987 Dec;84(24):9131–9134. doi: 10.1073/pnas.84.24.9131

Close linkage of the mouse and human CD3 gamma- and delta-chain genes suggests that their transcription is controlled by common regulatory elements.

H Saito 1, T Koyama 1, K Georgopoulos 1, H Clevers 1, W G Haser 1, T LeBien 1, S Tonegawa 1, C Terhorst 1
PMCID: PMC299706  PMID: 2827170

Abstract

Antigen receptors on the T-cell surface are noncovalently associated with at least four invariant polypeptide chains, CD3-gamma, -delta, -epsilon, and -zeta. The mouse CD3-gamma gene, consisting of seven exons, was found to be highly homologous to the CD3-delta gene described earlier. Both the high level of sequence homology and the exon/intron organization indicate that the CD3-gamma and -delta genes arose by gene duplication. Surprisingly, murine and human genomic DNA clones could be isolated that contained elements of both the CD3-gamma and CD3-delta genes. In fact, the putative transcription start site of the mouse CD3-gamma gene is less than 1.4 kilobases from the transcription initiation site of the mouse CD3-delta gene. Common elements that regulate the divergent transcription of the two genes are therefore proposed to be located in the intervening 1.4-kilobase DNA segment. This might contribute to the coordinate expression of the CD3-gamma and -delta genes during intrathymic maturation of T lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison J. P., Lanier L. L. Structure, function, and serology of the T-cell antigen receptor complex. Annu Rev Immunol. 1987;5:503–540. doi: 10.1146/annurev.iy.05.040187.002443. [DOI] [PubMed] [Google Scholar]
  2. Borst J., Coligan J. E., Oettgen H., Pessano S., Malin R., Terhorst C. The delta- and epsilon-chains of the human T3/T-cell receptor complex are distinct polypeptides. 1984 Nov 29-Dec 5Nature. 312(5993):455–458. doi: 10.1038/312455a0. [DOI] [PubMed] [Google Scholar]
  3. Farnham P. J., Abrams J. M., Schimke R. T. Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene. Proc Natl Acad Sci U S A. 1985 Jun;82(12):3978–3982. doi: 10.1073/pnas.82.12.3978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  5. Furley A. J., Mizutani S., Weilbaecher K., Dhaliwal H. S., Ford A. M., Chan L. C., Molgaard H. V., Toyonaga B., Mak T., van den Elsen P. Developmentally regulated rearrangement and expression of genes encoding the T cell receptor-T3 complex. Cell. 1986 Jul 4;46(1):75–87. doi: 10.1016/0092-8674(86)90861-5. [DOI] [PubMed] [Google Scholar]
  6. Gold D. P., Clevers H., Alarcon B., Dunlap S., Novotny J., Williams A. F., Terhorst C. Evolutionary relationship between the T3 chains of the T-cell receptor complex and the immunoglobulin supergene family. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7649–7653. doi: 10.1073/pnas.84.21.7649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gold D. P., van Dongen J. J., Morton C. C., Bruns G. A., van den Elsen P., Geurts van Kessel A. H., Terhorst C. The gene encoding the epsilon subunit of the T3/T-cell receptor complex maps to chromosome 11 in humans and to chromosome 9 in mice. Proc Natl Acad Sci U S A. 1987 Mar;84(6):1664–1668. doi: 10.1073/pnas.84.6.1664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldsmith M. R., Kafatos F. C. Developmentally regulated genes in silkmoths. Annu Rev Genet. 1984;18:443–487. doi: 10.1146/annurev.ge.18.120184.002303. [DOI] [PubMed] [Google Scholar]
  9. Haser W. G., Saito H., Koyama T., Tonegawa S. Cloning and sequencing of murine T3 gamma cDNA from a subtractive cDNA library. J Exp Med. 1987 Oct 1;166(4):1186–1191. doi: 10.1084/jem.166.4.1186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Karathanasis S. K., Oettgen P., Haddad I. A., Antonarakis S. E. Structure, evolution, and polymorphisms of the human apolipoprotein A4 gene (APOA4). Proc Natl Acad Sci U S A. 1986 Nov;83(22):8457–8461. doi: 10.1073/pnas.83.22.8457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kay R. J., Boissy R. J., Russnak R. H., Candido E. P. Efficient transcription of a Caenorhabditis elegans heat shock gene pair in mouse fibroblasts is dependent on multiple promoter elements which can function bidirectionally. Mol Cell Biol. 1986 Sep;6(9):3134–3143. doi: 10.1128/mcb.6.9.3134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koyama T., Hall L. R., Haser W. G., Tonegawa S., Saito H. Structure of a cytotoxic T-lymphocyte-specific gene shows a strong homology to fibrinogen beta and gamma chains. Proc Natl Acad Sci U S A. 1987 Mar;84(6):1609–1613. doi: 10.1073/pnas.84.6.1609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Krissansen G. W., Owen M. J., Verbi W., Crumpton M. J. Primary structure of the T3 gamma subunit of the T3/T cell antigen receptor complex deduced from cDNA sequences: evolution of the T3 gamma and delta subunits. EMBO J. 1986 Aug;5(8):1799–1808. doi: 10.1002/j.1460-2075.1986.tb04429.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  15. Mitchell P. J., Carothers A. M., Han J. H., Harding J. D., Kas E., Venolia L., Chasin L. A. Multiple transcription start sites, DNase I-hypersensitive sites, and an opposite-strand exon in the 5' region of the CHO dhfr gene. Mol Cell Biol. 1986 Feb;6(2):425–440. doi: 10.1128/mcb.6.2.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oettgen H. C., Pettey C. L., Maloy W. L., Terhorst C. A T3-like protein complex associated with the antigen receptor on murine T cells. Nature. 1986 Mar 20;320(6059):272–275. doi: 10.1038/320272a0. [DOI] [PubMed] [Google Scholar]
  17. Pettey C. L., Alarcon B., Malin R., Weinberg K., Terhorst C. T3-p28 is a protein associated with the delta and epsilon chains of the T cell receptor-T3 antigen complex during biosynthesis. J Biol Chem. 1987 Apr 5;262(10):4854–4859. [PubMed] [Google Scholar]
  18. Saito H., Kranz D. M., Takagaki Y., Hayday A. C., Eisen H. N., Tonegawa S. A third rearranged and expressed gene in a clone of cytotoxic T lymphocytes. Nature. 1984 Nov 1;312(5989):36–40. doi: 10.1038/312036a0. [DOI] [PubMed] [Google Scholar]
  19. Saito H., Kranz D. M., Takagaki Y., Hayday A. C., Eisen H. N., Tonegawa S. Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences. 1984 Jun 28-Jul 4Nature. 309(5971):757–762. doi: 10.1038/309757a0. [DOI] [PubMed] [Google Scholar]
  20. Samelson L. E., Harford J. B., Klausner R. D. Identification of the components of the murine T cell antigen receptor complex. Cell. 1985 Nov;43(1):223–231. doi: 10.1016/0092-8674(85)90027-3. [DOI] [PubMed] [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  23. Toyonaga B., Mak T. W. Genes of the T-cell antigen receptor in normal and malignant T cells. Annu Rev Immunol. 1987;5:585–620. doi: 10.1146/annurev.iy.05.040187.003101. [DOI] [PubMed] [Google Scholar]
  24. van den Elsen P., Bruns G., Gerhard D. S., Pravtcheva D., Jones C., Housman D., Ruddle F. A., Orkin S., Terhorst C. Assignment of the gene coding for the T3-delta subunit of the T3-T-cell receptor complex to the long arm of human chromosome 11 and to mouse chromosome 9. Proc Natl Acad Sci U S A. 1985 May;82(9):2920–2924. doi: 10.1073/pnas.82.9.2920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. van den Elsen P., Georgopoulos K., Shepley B. A., Orkin S., Terhorst C. Exon/intron organization of the genes coding for the delta chains of the human and murine T-cell receptor/T3 complex. Proc Natl Acad Sci U S A. 1986 May;83(9):2944–2948. doi: 10.1073/pnas.83.9.2944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. van den Elsen P., Shepley B. A., Borst J., Coligan J. E., Markham A. F., Orkin S., Terhorst C. Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex. 1984 Nov 29-Dec 5Nature. 312(5993):413–418. doi: 10.1038/312413a0. [DOI] [PubMed] [Google Scholar]
  27. van den Elsen P., Shepley B. A., Cho M., Terhorst C. Isolation and characterization of a cDNA clone encoding the murine homologue of the human 20K T3/T-cell receptor glycoprotein. Nature. 1985 Apr 11;314(6011):542–544. doi: 10.1038/314542a0. [DOI] [PubMed] [Google Scholar]

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