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. 1974 Aug;54(2):247–251. doi: 10.1172/JCI107759

Pinocytosis by Human Alveolar Macrophages COMPARISON OF SMOKERS AND NONSMOKERS

Henry Yeager Jr 1,2, Steven M Zimmet 1,2, Sorell L Schwartz 1,2
PMCID: PMC301551  PMID: 4858777

Abstract

Alveolar macrophages were obtained from human volunteers, smokers and nonsmokers, by bronchial lavage through a fiberoptic bronchoscope. Cells were incubated in a chemically defined medium containing [14C]sucrose (0.36 mM) and varying concentrations of rabbit serum. Pinocytosis was assessed by the cellular uptake of isotope over 30, 75, and 120-min periods. Pinocytic activity of smokers' cells was dependent on serum concentration but always less than the activity of nonsmokers' cells. The degree of pinocytosis by nonsmokers' cells was independent of serum concentration. It is concluded that the decreased level of pinocytic activity in smokers' alveolar macrophages as indicated by the uptake of sucrose in the presence of rabbit serum may represent a form of reticuloendothelial blockade.

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Selected References

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  1. Bolognesi D. P., Langlois A. J., Sverak L., Bonar R. A., Beard J. W. In vitro chick embryo cell response to strain MC29 avian leukosis virus. J Virol. 1968 Jun;2(6):576–586. doi: 10.1128/jvi.2.6.576-586.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CASARETT L. J., MILLEY P. S. ALVEOLAR REACTIVITY FOLLOWING INHALATION OF PARTICLES. Health Phys. 1964 Dec;10:1003–1011. doi: 10.1097/00004032-196412000-00019. [DOI] [PubMed] [Google Scholar]
  3. COHN Z. A., BENSON B. THE IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. II. THE INFLUENCE OF SERUM ON GRANULE FORMATION, HYDROLASE PRODUCTION, AND PINOCYTOSIS. J Exp Med. 1965 May 1;121:835–848. doi: 10.1084/jem.121.5.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen A. B., Cline M. J. The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristics. J Clin Invest. 1971 Jul;50(7):1390–1398. doi: 10.1172/JCI106622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohn Z. A., Ehrenreich B. A. The uptake, storage, and intracellular hydrolysis of carbohydrates by macrophages. J Exp Med. 1969 Jan 1;129(1):201–225. doi: 10.1084/jem.129.1.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Finklea J. F., Hasselblad V., Sandifer S. H., Hammer D. I., Lowrimore G. R. Cigarette smoking and acute non-influenzal respiratory disease in military cadets. Am J Epidemiol. 1971 Jun;93(6):457–462. doi: 10.1093/oxfordjournals.aje.a121279. [DOI] [PubMed] [Google Scholar]
  7. Finklea J. F., Sandifer S. H., Smith D. D. Cigarette smoking and epidemic influenza. Am J Epidemiol. 1969 Nov;90(5):390–399. doi: 10.1093/oxfordjournals.aje.a121084. [DOI] [PubMed] [Google Scholar]
  8. Finley T. N., Ladman A. J. Low yield of pulmonary surfactant in cigarette smokers. N Engl J Med. 1972 Feb 3;286(5):223–227. doi: 10.1056/NEJM197202032860501. [DOI] [PubMed] [Google Scholar]
  9. Finley T. N., Swenson E. W., Curran W. S., Huber G. L., Ladman A. J. Bronchopulmonary lavage in normal subjects and patients with obstructive lung disease. Ann Intern Med. 1967 Apr;66(4):651–658. doi: 10.7326/0003-4819-66-4-651. [DOI] [PubMed] [Google Scholar]
  10. Green G. M., Carolin D. The depressant effect of cigarette smoke on the in vitro antibacterial activity of alveolar macrophages. N Engl J Med. 1967 Feb 23;276(8):421–427. doi: 10.1056/NEJM196702232760801. [DOI] [PubMed] [Google Scholar]
  11. Green G. M. The J. Burns Amberson Lecture--in defense of the lung. Am Rev Respir Dis. 1970 Nov;102(5):691–703. doi: 10.1164/arrd.1970.102.5.691. [DOI] [PubMed] [Google Scholar]
  12. Harris J. O., Swenson E. W., Johnson J. E., 3rd Human alveolar macrophages: comparison of phagocytic ability, glucose utilization, and ultrastructure in smokers and nonsmokers. J Clin Invest. 1970 Nov;49(11):2086–2096. doi: 10.1172/JCI106426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ikeda S., Yanai N., Ishikawa S. Flexible bronchofiberscope. Keio J Med. 1968 Mar;17(1):1–16. doi: 10.2302/kjm.17.1. [DOI] [PubMed] [Google Scholar]
  14. Jeunet F. S., Cain W. A., Good R. A. Reticuloendothelial function in the isolated perfused liver. 3. Phagocytosis of Salmonella typhosa and Brucella melitensis and the blockade of the reticuloendothelial system. J Reticuloendothel Soc. 1969 Aug;6(4):391–410. [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Mann P. E., Cohen A. B., Finley T. N., Ladman A. J. Alveolar macrophages. Structural and functional differences between nonsmokers and smokers of marijuana and tobacco. Lab Invest. 1971 Aug;25(2):111–120. [PubMed] [Google Scholar]
  17. Martin R. R. Altered morphology and increased acid hydrolase content of pulmonary macrophages from cigarette smokers. Am Rev Respir Dis. 1973 Apr;107(4):596–601. doi: 10.1164/arrd.1973.107.4.596. [DOI] [PubMed] [Google Scholar]
  18. Nyberg E., Dingle J. T. Endocytosis of sucrose and other sugars by cells in culture. Exp Cell Res. 1970 Nov;63(1):43–52. doi: 10.1016/0014-4827(70)90329-0. [DOI] [PubMed] [Google Scholar]
  19. Parnell J. L., Anderson D. O., Kinnis C. Cigarette smoking and respiratory infections in a class of student nurses. N Engl J Med. 1966 May 5;274(18):979–984. doi: 10.1056/NEJM196605052741801. [DOI] [PubMed] [Google Scholar]
  20. Pratt S. A., Finley T. N., Smith M. H., Ladman A. J. A comparison of alveolar macrophages and pulmonary surfactant(?) obtained from the lungs of human smokers and nonsmokers by endobronchial lavage. Anat Rec. 1969 Apr;163(4):497–507. doi: 10.1002/ar.1091630402. [DOI] [PubMed] [Google Scholar]
  21. Pratt S. A., Smith M. H., Ladman A. J., Finley T. N. The ultrastructure of alveolar macrophages from human cigarette smokers and nonsmokers. Lab Invest. 1971 May;24(5):331–338. [PubMed] [Google Scholar]
  22. Robin E. D., Smith J. D., Tanser A. R., Adamson J. S., Millen J. E., Packer B. Ion and macromolecular transport in the alveolar macrophage. Biochim Biophys Acta. 1971 Jul 6;241(1):117–128. doi: 10.1016/0005-2736(71)90310-5. [DOI] [PubMed] [Google Scholar]
  23. Schwartz S. L., Hayes J. R., Ide R. S., Johnson C. B., Doolan P. D. Studies of the nephrotoxicity of ethylenediaminetetraacetic acid. Biochem Pharmacol. 1966 Mar;15(3):377–389. doi: 10.1016/0006-2952(66)90309-1. [DOI] [PubMed] [Google Scholar]
  24. Schwartz S. L., Johnson C. B. Pinocytosis as the cause of sucrose nephrosis. Nephron. 1971;8(3):246–254. doi: 10.1159/000179925. [DOI] [PubMed] [Google Scholar]
  25. WATTIAUX R., WATTIAUX-DECONINCK S., RUTGEERTS M. J., TULKENS P. INFLUENCE OF THE INJECTION OF A SUCROSE SOLUTION ON THE PROPERTIES OF RAT-LIVER LYSOSOMES. Nature. 1964 Aug 15;203:757–758. doi: 10.1038/203757a0. [DOI] [PubMed] [Google Scholar]
  26. Wagner R., Rosenberg M., Estensen R. Endocytosis in Chang liver cells. Quantitation by sucrose- 3 H uptake and inhibition by cytochalasin B. J Cell Biol. 1971 Sep;50(3):804–817. doi: 10.1083/jcb.50.3.804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yeager H., Jr Alveolar cells: depression effect of cigarette smoke on protein synthesis. Proc Soc Exp Biol Med. 1969 May;131(1):247–250. doi: 10.3181/00379727-131-33851. [DOI] [PubMed] [Google Scholar]

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