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. 1975 May;55(5):903–907. doi: 10.1172/JCI108018

Extractable nuclear antigen effect on the DNA anti-DNA reaction and NZB/NZW mouse nephritis.

A D Morris, C Littleton, L C Corman, J Esterly, G C Sharp
PMCID: PMC301832  PMID: 1079029

Abstract

Extractable nuclear antigen (ENA) is composed of at least two components, one a ribonucleo-protein sensitive to ribonuclease or heat and the other a protein. Antibodies to ENA are associated with a relatively benign clinical course in patients with systemic lupus erythematosus (SLE) in which DNA anti DNA complexes are thought pathogenic. The effect of ENA and anti-ENA on DNA anti-DNA reactions in vitro was studied. ENA effectively inhibited an anti-DNA hemagglutination reaction but no effect was found on binding of radioactive DNA or on the anti-hemocyanin hemagglutination reaction. The inhibitory effect was not abolished by yeast ribonuclease (RNase), heating, or DNase. Anti-ENA HAD NO EFFECT ON ANTI-DNA hemagglutination. In vivo, ENA altered the NZB/NZW mouse nephritis thought to be a model for human SLE nephritis. These results suggest the possiblity of a role for ENA in alteration of diseases due to pathogenic DNA anti-DNA complexes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLANSMITH M., GOIHMAN-YAHR M., BUELL D. N. DEMONSTRATION OF GAMMA-2 GLOBULIN IN HUMAN SKIN. J Allergy. 1964 Jul-Aug;35:313–321. doi: 10.1016/0021-8707(64)90094-2. [DOI] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Germuth F. G., Jr, Senterfit L. B., Dreesman G. R. Immune complex disease. V. The nature of the circulating complexes associated with glomerular alterations in the chronic BSA-rabbit system. Johns Hopkins Med J. 1972 Jun;130(6):344–357. [PubMed] [Google Scholar]
  4. Hahn B. H., Sharp G. C., Irvin W. S., Kantor O. S., Gardner C. A., Bagby M. K., Perry H. M., Jr, Osterland C. K. Immune responses to hydralazine and nuclear antigens in hydralazine-induced lupus erythematosus. Ann Intern Med. 1972 Mar;76(3):365–374. doi: 10.7326/0003-4819-76-3-365. [DOI] [PubMed] [Google Scholar]
  5. Hamburger M., Friedlander L., Barland P. Interactions of extractable nuclear antigen (ENA) and double stranded DNA. Arthritis Rheum. 1974 Jul-Aug;17(4):469–475. doi: 10.1002/art.1780170418. [DOI] [PubMed] [Google Scholar]
  6. Koffler D., Agnello V., Winchester R., Kunkel H. G. The occurrence of single-stranded DNA in the serum of patients with systemic lupus erythematosus and other diseases. J Clin Invest. 1973 Jan;52(1):198–204. doi: 10.1172/JCI107165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lambert P. H., Dixon F. J. Pathogenesis of the glomerulonephritis of NZB/W mice. J Exp Med. 1968 Mar 1;127(3):507–522. doi: 10.1084/jem.127.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pincus T. Immunochemical conditions affecting the measurement of DNA antibodies using ammonium sulfate precipitation. Arthritis Rheum. 1971 Sep-Oct;14(5):623–630. doi: 10.1002/art.1780140509. [DOI] [PubMed] [Google Scholar]
  9. Reichlin M., Mattioli M. Correlation of a precipitin reaction to an RNAprotein antigen and a low prevalence of nephritis in patients with systemic lupus erythematosus. N Engl J Med. 1972 Apr 27;286(17):908–911. doi: 10.1056/NEJM197204272861702. [DOI] [PubMed] [Google Scholar]
  10. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
  11. Sharp G. C., Irvin W. S., LaRoque R. L., Velez C., Daly V., Kaiser A. D., Holman H. R. Association of autoantibodies to different nuclear antigens with clinical patterns of rheumatic disease and responsiveness to therapy. J Clin Invest. 1971 Feb;50(2):350–359. doi: 10.1172/JCI106502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sharp G. C., Irvin W. S., Tan E. M., Gould R. G., Holman H. R. Mixed connective tissue disease--an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA). Am J Med. 1972 Feb;52(2):148–159. doi: 10.1016/0002-9343(72)90064-2. [DOI] [PubMed] [Google Scholar]

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