Abstract
Erythrocytes containing pig liver uricase have been prepared by hypotonic hemolysis in the presence of the enzyme. Uricase is shown to be active within the erythrocytes and to degrade uric acid as rapidly as it enters the cells when high intracellular enzyme concentrations are employed. The kinetics and characteristics of uric acid entry are shown to be the same for hemolysed and normal erythrocytes. At physiological concentrations of uric acid, loaded erythrocytes can degrade a maximum of about 21 mumol uric acid/liter erythrocytes per min. The possible application of enzyme-loaded erythrocytes to medicine is discussed.
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Selected References
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- BAUM H., HUBSCHER G., MAHLER H. R. Studies on uricase. II. The enzyme-substrate complex. Biochim Biophys Acta. 1956 Dec;22(3):514–527. doi: 10.1016/0006-3002(56)90062-2. [DOI] [PubMed] [Google Scholar]
- HOFFMAN J. F. The active transport of sodium by ghosts of human red blood cells. J Gen Physiol. 1962 May;45:837–859. doi: 10.1085/jgp.45.5.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ihler G. M., Glew R. H., Schnure F. W. Enzyme loading of erythrocytes. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2663–2666. doi: 10.1073/pnas.70.9.2663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LASSEN U. V. Kinetics of uric acid transport in human erythrocytes. Biochim Biophys Acta. 1961 Nov 11;53:557–569. doi: 10.1016/0006-3002(61)90215-3. [DOI] [PubMed] [Google Scholar]
- LASSEN U. V., OVERGAARD-HANSEN K. Hypoxanthine as inhibitor of uric acid transport in human erythrocytes. Biochim Biophys Acta. 1962 Feb 12;57:111–117. doi: 10.1016/0006-3002(62)91085-5. [DOI] [PubMed] [Google Scholar]
- MAHLER H. R., HUBSCHER G., BAUM R. Studies on uricase. I. Preparation, purification, and properties of a cuproprotein. J Biol Chem. 1955 Oct;216(2):625–641. [PubMed] [Google Scholar]
- Rifkind R. A. Destruction of injured red cells in vivo. Am J Med. 1966 Nov;41(5):711–723. doi: 10.1016/0002-9343(66)90032-5. [DOI] [PubMed] [Google Scholar]
- SCHRIER S. L. Studies of the metabolism of human erythrocyte membranes. J Clin Invest. 1963 Jun;42:756–766. doi: 10.1172/JCI104768. [DOI] [PMC free article] [PubMed] [Google Scholar]