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. 1975 Oct;56(4):808–813. doi: 10.1172/JCI108159

Comparison of agents producing a neutrophilic leukocytosis in man. Hydrocortisone, prednisone, endotoxin, and etiocholanolone.

D C Dale, A S Fauci, I V Guerry D, S M Wolff
PMCID: PMC301935  PMID: 1159089

Abstract

To study the potential application of glucocorticosteroid administration for the measurement of the bone marrow neutrophil reserve response, blood neutrophil count changes were measured in normal subjects after the administration of intravenous hydrocortisone (25, 50, 100, 200, and 400 mg) and oral prednisone (5, 10, 20, 40, and 80 mg). The upper three doses of both steroids increased the blood neutrophil count by approximately 4,000 cells/mm3. The neutrophilia occurring after hydrocortisone (200 mg) and/or prednisone (40 mg) was compared with that observed after endotoxin (0.8 ng/kg) and etiocholanolone (0.1 mg/kg) in 14 normal subjects, 7 patients with Wegener's granulomatosis on cyclophosphamide therapy and 10 patients with chronic idiopathic neutropenia. The normal responses (mean increase of blood neutrophils/mm3 above base line +/- 1 SEM) were: hydrocortisone 4,220 +/- 320, prednisone 4,610 +/- 360, endotoxin 6,060 +/- 880, and etiocholanolone 3,780 +/- 440. In the patient studies, etiocholanolone gave the smallest mean responses, but, in general, the results were similar for all agents. These data indicate that these glucocorticosteroids can be used as equivalent agents to endotoxin and etiocholanolone for measuring the neutrophil reserve response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATHENS J. W., HAAB O. P., RAAB S. O., MAUER A. M., ASHENBRUCKER H., CARTWRIGHT G. E., WINTROBE M. M. Leukokinetic studies. IV. The total blood, circulating and marginal granulocyte pools and the granulocyte turnover rate in normal subjects. J Clin Invest. 1961 Jun;40:989–995. doi: 10.1172/JCI104338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnett C. W. THE UNAVOIDABLE ERROR IN THE DIFFERENTIAL COUNT OF THE LEUKOCYTES OF THE BLOOD. J Clin Invest. 1933 Jan;12(1):77–85. doi: 10.1172/JCI100496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop C. R., Athens J. W., Boggs D. R., Warner H. R., Cartwright G. E., Wintrobe M. M. Leukokinetic studies. 13. A non-steady-state kinetic evaluation of the mechanism of cortisone-induced granulocytosis. J Clin Invest. 1968 Feb;47(2):249–260. doi: 10.1172/JCI105721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bishop C. R., Rothstein G., Ashenbrucker H. E., Athens J. W. Leukokinetic studies. XIV. Blood neutrophil kinetics in chronic, steady-state neutropenia. J Clin Invest. 1971 Aug;50(8):1678–1689. doi: 10.1172/JCI106657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CRADDOCK C. G., Jr, PERRY S., VENTZKE L. E., LAWRENCE J. S. Evaluation of marrow granulocytic reserves in normal and disease states. Blood. 1960 Jun;15:840–855. [PubMed] [Google Scholar]
  6. Cream J. J. Prednisolone-induced granulocytosis. Br J Haematol. 1968 Sep;15(3):259–267. doi: 10.1111/j.1365-2141.1968.tb01537.x. [DOI] [PubMed] [Google Scholar]
  7. Dale D. C., Fauci A. S., Wolff S. M. Alternate-day prednisone. Leukocyte kinetics and susceptibility to infections. N Engl J Med. 1974 Nov 28;291(22):1154–1158. doi: 10.1056/NEJM197411282912203. [DOI] [PubMed] [Google Scholar]
  8. Dale D. C., Wolff S. M. Studies of the neutropenia of acute malaria. Blood. 1973 Feb;41(2):197–206. [PubMed] [Google Scholar]
  9. Deinard A. S., Fortuny I. E., Theologides A., Anderson G. L., Boen J., Kennedy B. J. Studies on the neutropenia of cancer chemotherapy. Cancer. 1974 May;33(5):1210–1218. doi: 10.1002/1097-0142(197405)33:5<1210::aid-cncr2820330503>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  10. Godwin H. A., Zimmerman T. S., Kimball H. R., Wolff S. M., Perry S. The effect of etiocholanolone on the entry of granulocytes into the peripheral blood. Blood. 1968 Apr;31(4):461–470. [PubMed] [Google Scholar]
  11. HERION J. C., WALKER R. I., HERRING W. B., PALMER J. G. EFFECTS OF ENDOTOXIN AND NITROGEN MUSTARD ON LEUKOCYTE KINETICS. Blood. 1965 Apr;25:522–540. [PubMed] [Google Scholar]
  12. Kimball H. R., Vogel J. M., Perry S., Wolff S. M. Quantitative aspects of pyrogenic and hematologic responses to etiocholanolone in man. J Lab Clin Med. 1967 Mar;69(3):415–427. [PubMed] [Google Scholar]
  13. Kimball H. R., Wolff S. M., Talal N., Plotz P. H., Decker J. L. Marrow granulocyte reserves in the rheumatic diseases. Arthritis Rheum. 1973 May-Jun;16(3):345–352. doi: 10.1002/art.1780160308. [DOI] [PubMed] [Google Scholar]
  14. MCFARLAND W., LIBRE E. P. ABNORMAL LEUKOCYTE RESPONSE IN ALCOHOLISM. Ann Intern Med. 1963 Dec;59:865–877. doi: 10.7326/0003-4819-59-6-865. [DOI] [PubMed] [Google Scholar]
  15. MECHANIC R. C., FREI E., 3rd, LANDY M., SMITH W. W. Quantitative studies of human leukocytic and febrile response to single and repeated doses of purified bacterial endotoxin. J Clin Invest. 1962 Jan;41:162–172. doi: 10.1172/JCI104459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Paulus H. E., Okun R., Calabro J. J. Immunosuppressive therapy: guidelines for drug dosage. Depression of bone marrow granulocyte reserves in systemic lupus erythematosus. Arthritis Rheum. 1972 Jan-Feb;15(1):29–35. doi: 10.1002/art.1780150105. [DOI] [PubMed] [Google Scholar]
  17. Perry S. Appraisal of myelopoiesis. N Engl J Med. 1971 Jul 22;285(4):214–217. doi: 10.1056/NEJM197107222850407. [DOI] [PubMed] [Google Scholar]
  18. QUITTNER H., WALD N., SUSSMAN L. N., ANTOPOL W. The effect of massive doses of cortisone on the peripheral blood and bone marrow of the mouse. Blood. 1951 Jun;6(6):513–521. [PubMed] [Google Scholar]
  19. SOYLEMEZOGLU B., WELLS J. A. Comparison of leukocyte response of ACTH and bacterial pyrogen. Proc Soc Exp Biol Med. 1951 May;77(1):43–47. doi: 10.3181/00379727-77-18672. [DOI] [PubMed] [Google Scholar]
  20. THATCHER L. G., SMITH N. J. Granulocyte responses of children to bacterial endotoxin. J Pediatr. 1963 Apr;62:484–494. doi: 10.1016/s0022-3476(63)80005-0. [DOI] [PubMed] [Google Scholar]
  21. Vogel J. M., Kimball H. R., Wolff S. M., Perry S. Etiocholanolone in the evaluation of marrow reserves in patients receiving cytotoxic agents. Ann Intern Med. 1967 Dec;67(6):1226–1238. doi: 10.7326/0003-4819-67-6-1226. [DOI] [PubMed] [Google Scholar]
  22. WOLFF S. M., RUBENSTEIN M., MULHOLLAND J. H., ALLING D. W. COMPARISON OF HEMATOLOGIC AND FEBRILE RESPONSE TO ENDOTOXIN IN MAN. Blood. 1965 Aug;26:190–201. [PubMed] [Google Scholar]

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