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. 1975 Nov;56(5):1189–1197. doi: 10.1172/JCI108196

Effect of corticosteroids on the human monocyte IgG and complement receptors.

A D Schreiber, J Parsons, P McDermott, R A Cooper
PMCID: PMC301983  PMID: 1184745

Abstract

A quantitative in vitro assay was employed to directly assess the effect of corticosteroids on the IgG and complement receptor function of human mononuclear phagocytic cells. In this system corticosteroids were solubilized with cholesterol-phospholipid sonicated dispersions before exposure to mononuclear cells. Solubilized corticosteroids at concentrations between 10(-4) and 10(-3) M inhibited both IgG and complement receptor activity in a dose-response fashion. Inhibition was dependent upon the time of interaction of the mononuclear cells with corticosteroids and was half-maximal by 15 min. The inhibitory effect at all concentrations of hydrocortisone was partially overcome by increasing the number of IgG molecules per erythrocyte. Hydrocortisone also inhibited the binding of erythrocytes coated with both IgG and C3, despite the fact that when both were on the erythrocyte surface a synergistic effect on binding to mononuclear cells was observed. At the steroid concentrations employed, the capacity of mononuclear cells to exclude trypan blue and to take up latex particles and neutral red was unaffected. Mineralocorticoids also inhibited receptor activity, but the sex hormones were less effective. These studies demonstrate an effect of steroid hormones on cell membrane receptor function, and they suggest that an inhibition of the recognition system for IgG and C3 in vivo may explain, in part, the effect of corticosteroids in man.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson J. P., Frank M. M. Complement-independent clearance of IgG-sensitized erythrocytes: inhibition by cortisone. Blood. 1974 Nov;44(5):629–637. [PubMed] [Google Scholar]
  2. Atkinson J. P., Frank M. M. Studies on the in vivo effects of antibody. Interaction of IgM antibody and complement in the immune clearance and destruction of erythrocytes in man. J Clin Invest. 1974 Aug;54(2):339–348. doi: 10.1172/JCI107769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Atkinson J. P., Schreiber A. D., Frank M. M. Effects of corticosteroids and splenectomy on the immune clearance and destruction of erythrocytes. J Clin Invest. 1973 Jun;52(6):1509–1517. doi: 10.1172/JCI107325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BILBEY D. L., NICOL T. Effect of various natural steroids on the phagocytic activity of the reticuloendothelial system. Nature. 1958 Sep 6;182(4636):674–674. doi: 10.1038/182674a0. [DOI] [PubMed] [Google Scholar]
  5. Bokisch V. A., Sobel A. T. Receptor for the fourth component of complement on human B lymphocytes and cultured human lymphoblastoid cells. J Exp Med. 1974 Nov 1;140(5):1336–1347. doi: 10.1084/jem.140.5.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Borsos T., Colten H. R., Spalter J. S., Rogentine N., Rapp H. J. The C'la fixation and transfer test: examples of its applicability to the detection and enumeration of antigens and antibodies at cell surfaces. J Immunol. 1968 Sep;101(3):392–398. [PubMed] [Google Scholar]
  7. Borsos T., Leonard E. J. Detection of bound C3 by a new immunochemical methods. J Immunol. 1971 Sep;107(3):766–771. [PubMed] [Google Scholar]
  8. Borsos T., Rapp H. J. Hemolysin titration based on fixation of the activated first component of complement: evidence that one molecule of hemolysin suffices to sensitize an erythrocyte. J Immunol. 1965 Sep;95(3):559–566. [PubMed] [Google Scholar]
  9. Borsos T., Rapp H. J. Immune hemolysis: a simplified method for the preparation of EAC'4 with guinea pig or with human complement. J Immunol. 1967 Aug;99(2):263–268. [PubMed] [Google Scholar]
  10. Bruckdorfer K. R., Edwards P. A., Green C. Properties of aqueous dispersions of phospholipid and cholesterol. Eur J Biochem. 1968 May;4(4):506–511. doi: 10.1111/j.1432-1033.1968.tb00241.x. [DOI] [PubMed] [Google Scholar]
  11. Bruckdorfer K. R., Graham J. M., Green C. The incorporation of steroid molecules into lecithin sols, beta-lipoproteins and cellular membranes. Eur J Biochem. 1968 May;4(4):512–518. doi: 10.1111/j.1432-1033.1968.tb00242.x. [DOI] [PubMed] [Google Scholar]
  12. Claman H. N. Corticosteroids and lymphoid cells. N Engl J Med. 1972 Aug 24;287(8):388–397. doi: 10.1056/NEJM197208242870806. [DOI] [PubMed] [Google Scholar]
  13. Cooper R. A., Arner E. C., Wiley J. S., Shattil S. J. Modification of red cell membrane structure by cholesterol-rich lipid dispersions. A model for the primary spur cell defect. J Clin Invest. 1975 Jan;55(1):115–126. doi: 10.1172/JCI107901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Devenuto F., Ligon D. F., Friedrichsen D. H., Wilson H. L. Human erythrocyte membrane. Uptake of progesterone and chemical alterations. Biochim Biophys Acta. 1969 Oct 14;193(1):36–47. doi: 10.1016/0005-2736(69)90056-x. [DOI] [PubMed] [Google Scholar]
  15. Graham J. M., Green C. The binding of hormones and related compounds by normal and cholesterol-depleted plasma membranes of rat liver. Biochem Pharmacol. 1969 Feb;18(2):493–502. doi: 10.1016/0006-2952(69)90225-1. [DOI] [PubMed] [Google Scholar]
  16. Huber H., Polley M. J., Linscott W. D., Fudenberg H. H., Müller-Eberhard H. J. Human monocytes: distinct receptor sites for the third component of complement and for immunoglobulin G. Science. 1968 Dec 13;162(3859):1281–1283. doi: 10.1126/science.162.3859.1281. [DOI] [PubMed] [Google Scholar]
  17. KAPLAN M. E., JANDL J. H. Inhibition of red cell sequestration by cortisone. J Exp Med. 1961 Dec 1;114:921–937. doi: 10.1084/jem.114.6.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. KAPLOW L. S. SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE. Blood. 1965 Aug;26:215–219. [PubMed] [Google Scholar]
  19. Kellaway I. W., Saunders L. The solubilization of some steroids by phosphatidyl choline and lysophosphatidyl choline. Biochim Biophys Acta. 1967 Aug 8;144(1):145–148. doi: 10.1016/0005-2760(67)90087-2. [DOI] [PubMed] [Google Scholar]
  20. LoBuglio A. F., Cotran R. S., Jandl J. H. Red cells coated with immunoglobulin G: binding and sphering by mononuclear cells in man. Science. 1967 Dec 22;158(3808):1582–1585. doi: 10.1126/science.158.3808.1582. [DOI] [PubMed] [Google Scholar]
  21. Logue G. L., Rosse W. F., Gockerman J. P. Measurement of the third component of complement bound to red blood cells in patients with the cold agglutinin syndrome. J Clin Invest. 1973 Feb;52(2):493–501. doi: 10.1172/JCI107206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  23. Mantovani B., Rabinovitch M., Nussenzweig V. Phagocytosis of immune complexes by macrophages. Different roles of the macrophage receptor sites for complement (C3) and for immunoglobulin (IgG). J Exp Med. 1972 Apr 1;135(4):780–792. doi: 10.1084/jem.135.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Mollison P. L. The role of complement in antibody-mediated red-cell destruction. Br J Haematol. 1970 Mar;18(3):249–255. doi: 10.1111/j.1365-2141.1970.tb01440.x. [DOI] [PubMed] [Google Scholar]
  25. PETERSON R. E., WYNGAARDEN J. B., GUERRA S. L., BRODIE B. B., BUNIM J. J. The physiological disposition and metabolic fate of hydrocortisone in man. J Clin Invest. 1955 Dec;34(12):1779–1794. doi: 10.1172/JCI103233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rinehart J. J., Balcerzak S. P., Sagone A. L., LoBuglio A. F. Effects of corticosteroids on human monocyte function. J Clin Invest. 1974 Dec;54(6):1337–1343. doi: 10.1172/JCI107880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rinehart J. J., Sagone A. L., Balcerzak S. P., Ackerman G. A., LoBuglio A. F. Effects of corticosteroid therapy on human monocyte function. N Engl J Med. 1975 Jan 30;292(5):236–241. doi: 10.1056/NEJM197501302920504. [DOI] [PubMed] [Google Scholar]
  28. Ross G. D., Polley M. J., Rabellino E. M., Grey H. M. Two different complement receptors on human lymphocytes. One specific for C3b and one specific for C3b inactivator-cleaved C3b. J Exp Med. 1973 Oct 1;138(4):798–811. doi: 10.1084/jem.138.4.798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Rosse W. F. Quantitative immunology of immune hemolytic anemia. I. The fixation of C1 by autoimmune antibody and heterologous anti-IgG antibody. J Clin Invest. 1971 Apr;50(4):727–733. doi: 10.1172/JCI106543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Rosse W. F. Quantitative immunology of immune hemolytic anemia: II. The relationship of cell-bound antibody to hemolysis and the effect of treatment. J Clin Invest. 1971 Apr;50(4):734–743. doi: 10.1172/JCI106544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Ruddy S., Austen K. F. A stoichiometric assay for the fourth component of complement in whole human serum using EAC'la-gp and functionally pure human second component. J Immunol. 1967 Dec;99(6):1162–1172. [PubMed] [Google Scholar]
  32. Ruddy S., Austen K. F. C3b inactivator of man. II. Fragments produced by C3b inactivator cleavage of cell-bound or fluid phase C3b. J Immunol. 1971 Sep;107(3):742–750. [PubMed] [Google Scholar]
  33. Schreiber A. D., Austen K. F. Hageman factor-independent fibrinolytic pathway. Clin Exp Immunol. 1974 Aug;17(4):587–600. [PMC free article] [PubMed] [Google Scholar]
  34. Schreiber A. D., Frank M. M. Role of antibody and complement in the immune clearance and destruction of erythrocytes. I. In vivo effects of IgG and IgM complement-fixing sites. J Clin Invest. 1972 Mar;51(3):575–582. doi: 10.1172/JCI106846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Sessa G., Weissmann G. Phospholipid spherules (liposomes) as a model for biological membranes. J Lipid Res. 1968 May;9(3):310–318. [PubMed] [Google Scholar]
  36. Shattil S. J., Anaya-Galindo R., Bennett J., Colman R. W., Cooper R. A. Platelet hypersensitivity induced by cholesterol incorporation. J Clin Invest. 1975 Mar;55(3):636–643. doi: 10.1172/JCI107971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Stossel T. P. Phagocytosis (third of three parts). N Engl J Med. 1974 Apr 11;290(15):833–839. doi: 10.1056/NEJM197404112901506. [DOI] [PubMed] [Google Scholar]
  38. Vernon-Roberts B. The effects of steroid hormones on macrophage activity. Int Rev Cytol. 1969;25:131–159. doi: 10.1016/s0074-7696(08)60202-8. [DOI] [PubMed] [Google Scholar]
  39. WEISSMANN G., THOMAS L. Studies on lysosomes. II. The effect of cortisone on the release of acid hydrolases from a large granule fraction of rabbit liver induced by an excess of vitamin A. J Clin Invest. 1963 May;42:661–669. doi: 10.1172/JCI104757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. WILLMER E. N. Steroids and cell surfaces. Biol Rev Camb Philos Soc. 1961 Aug;36:368–398. doi: 10.1111/j.1469-185x.1961.tb01295.x. [DOI] [PubMed] [Google Scholar]
  41. Webel M. L., Ritts R. E., Jr, Taswell H. F., Danadio J. V., Jr, Woods J. E. Cellular immunity after intravenous administration of methylprednisolone. J Lab Clin Med. 1974 Mar;83(3):383–392. [PubMed] [Google Scholar]
  42. Weston W. L., Claman H. N., Krueger G. G. Site of action of cortisol in cellular immunity. J Immunol. 1973 Mar;110(3):880–883. [PubMed] [Google Scholar]
  43. van Furth R. Origin and kinetics of monocytes and macrophages. Semin Hematol. 1970 Apr;7(2):125–141. [PubMed] [Google Scholar]

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