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. 1973 Aug;52(8):1985–1993. doi: 10.1172/JCI107383

Response of Plasma Histaminase Activity to Small Doses of Heparin in Normal Subjects and Patients with Hyperlipoproteinemia

Stephen B Baylin 1,2, Michael A Beaven 1,2, Ronald M Krauss 1,2, Harry R Keiser 1,2
PMCID: PMC302481  PMID: 4198195

Abstract

The release of histaminase activity in plasma after small intravenous of heparin was studied in 85 normal subjects and patients. In normal subjects, plasma histaminase activity (basal level, 1.7±0.1 U/ml, mean ±SEM) increased 1.6±0.2 U/ml after 10 U of heparin/kg, 8.5±2.4 U/ml after 20 U/kg, and 33±4.9 U/ml after 75 U/kg. The extent of the increase varied widely among individuals but in a particular individual the response was constant and dose-dependent. Histaminase activity rose to peak levels within 7-15 min and then declined exponentially with a half-life of 40-120 min. This pattern of response was also observed in two patients with the histaminase-producing tumor, medullary carcinoma of the thyroid. A significantly reduced response was observed, however, in 14 patients with type I hyperlipoproteinemia, a disorder in which high plasma triglyceride levels are associated with low postheparin plasma lipolytic activity. After 10 U heparin/kg, plasma histamine activity increased 0.5±0.2 U/ml, and after 75 U heparin/kg, 10.9±5.6 U/ml. In contrast, in 27 patients with other types of hyperlipoproteinemia in whom postheparin lipolytic activity was normal, the increase (2.4±0.6 U/ml) in plasma histaminase activity after 10 U heparin/kg was not significantly different from that of normal subjects. The reduced response of the plasma histaminase activity to heparin in patients with type I hyperlipoproteinemia did not appear to be due to the presence of lipemia or to an inhibitor of the enzyme in plasma. These findings suggest that many patients with type I hyperlipoproteinemia may have deficient release of both lipolytic and histaminase activities into plasma after heparin administration.

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1988

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOBERG J., CARLSON L. A. DETERMINATION OF HEPARIN-INDUCED LIPOPROTEIN LIPASE ACTIVITY IN HUMAN PLASMA. Clin Chim Acta. 1964 Nov;10:420–427. doi: 10.1016/0009-8981(64)90171-8. [DOI] [PubMed] [Google Scholar]
  2. BROWN R. K., BOYLE E., ANFINSEN C. B. The enzymatic transformation of lipoproteins. J Biol Chem. 1953 Sep;204(1):423–434. [PubMed] [Google Scholar]
  3. Baylin S. B., Beaven M. A., Buja L. M., Keiser H. R. Histaminase activity: a biochemical marker for medullary carcinoma of the thyroid. Am J Med. 1972 Dec;53(6):723–733. doi: 10.1016/0002-9343(72)90189-1. [DOI] [PubMed] [Google Scholar]
  4. Baylin S. B., Beaven M. A., Engelman K., Sjoerdsma A. Elevated histaminase activity in medullary carcinoma of the thyroid gland. N Engl J Med. 1970 Dec 3;283(23):1239–1244. doi: 10.1056/NEJM197012032832301. [DOI] [PubMed] [Google Scholar]
  5. Baylin S. B., Beaven M. A., Keiser H. R., Tashjian A. H., Jr, Melvin K. E. Serum histaminase and calcitonin levels in medullary carcinoma of the thyroid. Lancet. 1972 Feb 26;1(7748):455–458. doi: 10.1016/s0140-6736(72)90120-1. [DOI] [PubMed] [Google Scholar]
  6. Beaven M. A., Jacobsen S. A new assay for histaminase activity: measurement of tritiated water from beta (side chain label)-H 3-histamine. J Pharmacol Exp Ther. 1971 Jan;176(1):52–64. [PubMed] [Google Scholar]
  7. Biale Y., Shafrir E. Lipolytic activity toward tri- and monoglycerides in postheparin plasma. Clin Chim Acta. 1969 Mar;23(3):413–419. doi: 10.1016/0009-8981(69)90341-6. [DOI] [PubMed] [Google Scholar]
  8. Dahlbäck O., Hansson R., Tibbling G., Tryding N. The effect of heparin on diamine oxidase and lipoprotein lipase in human lymph and blood plasma. Scand J Clin Lab Invest. 1968;21(1):17–25. doi: 10.3109/00365516809076972. [DOI] [PubMed] [Google Scholar]
  9. Fredrickson D. S., Levy R. I., Lees R. S. Fat transport in lipoproteins--an integrated approach to mechanisms and disorders. N Engl J Med. 1967 Jan 19;276(3):148–contd. doi: 10.1056/NEJM196701192760305. [DOI] [PubMed] [Google Scholar]
  10. Giertz H., Hahn F. Mechanism of histaminase liberation in guinea pig anaphylaxis. Int Arch Allergy Appl Immunol. 1969;36(1):41–44. doi: 10.1159/000230719. [DOI] [PubMed] [Google Scholar]
  11. Glueck C. J., Kaplan A. P., Levy R. I., Greten H., Gralnick H., Fredrickson D. S. A new mechanism of exogenous hyperglyceridemia. Ann Intern Med. 1969 Dec;71(6):1051–1062. doi: 10.7326/0003-4819-71-6-1051. [DOI] [PubMed] [Google Scholar]
  12. Greten H., Levy R. I., Fredrickson D. S. A further characterization of lipoprotein lipase. Biochim Biophys Acta. 1968 Oct 22;164(2):185–194. doi: 10.1016/0005-2760(68)90145-8. [DOI] [PubMed] [Google Scholar]
  13. Greten H., Levy R. I., Fredrickson D. S. Evidence for separate monoglyceride hydrolase and triglyceride lipase in post-heparin human plasma. J Lipid Res. 1969 May;10(3):326–330. [PubMed] [Google Scholar]
  14. Hansson R. Diamine oxidase isoenzymes in human blood plasma. Scand J Clin Lab Invest. 1970 Jan;25(1):33–39. doi: 10.3109/00365517009046187. [DOI] [PubMed] [Google Scholar]
  15. Hansson R., Holmberg C. G., Tibbling G., Tryding N., Westling H., Wetterqvist H. Heparin-induced diamine oxidase increase in human blood plasma. Acta Med Scand. 1966 Nov;180(5):533–536. doi: 10.1111/j.0954-6820.1966.tb02866.x. [DOI] [PubMed] [Google Scholar]
  16. Hansson R., Thysell H. Diamine oxidase in blood plasma in some vertebrates and Anodonta cygnea before and after injection of heparin. Acta Physiol Scand. 1968 Dec;74(4):533–542. doi: 10.1111/j.1748-1716.1968.tb04265.x. [DOI] [PubMed] [Google Scholar]
  17. Hansson R., Thysell H. Heparin-induced increase of diamine oxidase in lymph and blood plasma of rat, guinea-pig and rabbit. Acta Physiol Scand. 1971 Feb;81(2):208–214. doi: 10.1111/j.1748-1716.1971.tb04893.x. [DOI] [PubMed] [Google Scholar]
  18. KORN E. D. Clearing factor, a heparin-activated lipoprotein lipase. I. Isolation and characterization of the enzyme from normal rat heart. J Biol Chem. 1955 Jul;215(1):1–14. [PubMed] [Google Scholar]
  19. Kobayashi Y., Kupelian J., Maudsley D. V. Release of diamine oxidase by heparin in the rat. Biochem Pharmacol. 1969 Jul;18(7):1585–1591. doi: 10.1016/0006-2952(69)90145-2. [DOI] [PubMed] [Google Scholar]
  20. Robinson D. S., Lovenberg W., Keiser H., Sjoerdsma A. Effects of drugs on human blood platelet and plasma amine oxidase activity in vitro and in vivo. Biochem Pharmacol. 1968 Jan;17(1):109–119. doi: 10.1016/0006-2952(68)90163-9. [DOI] [PubMed] [Google Scholar]
  21. SCHULER W. Zur Hemmung der Diaminooxydase (Histaminase). Experientia. 1952 Jun 15;8(6):230–232. doi: 10.1007/BF02170726. [DOI] [PubMed] [Google Scholar]
  22. SWANBERG H. Histaminase in pregnancy: with special reference to its origin and formation; an experimental and clinical study. Acta Physiol Scand Suppl. 1950;23(79):1–69. [PubMed] [Google Scholar]
  23. Southren A. L., Kobayashi Y., Jung W., Carmody N. C., Weingold A. B. In vitro production of diamine oxidase by the perfused human placenta. J Clin Endocrinol Metab. 1966 Sep;26(9):1005–1009. doi: 10.1210/jcem-26-9-1005. [DOI] [PubMed] [Google Scholar]
  24. Tryding N., Willert B. Determination of plasma diamine oxidase (histaminase) in clinical practice. A comparison between a biological method and a radiochemical micromethod. Scand J Clin Lab Invest. 1968;22(1):29–32. doi: 10.3109/00365516809160732. [DOI] [PubMed] [Google Scholar]
  25. Vogel W. C., Bierman E. L. Post-heparin serum lecithinase in man and its positional specificity. J Lipid Res. 1967 Jan;8(1):46–53. [PubMed] [Google Scholar]
  26. Vogel W. C., Brunzell J. D., Bierman E. L. A comparison of triglyceride, monoglyceride, and phospholipid substrates for post-heparin lipolytic activities from normal and hypertriglyceridemic subjects. Lipids. 1971 Nov;6(11):805–814. doi: 10.1007/BF02531209. [DOI] [PubMed] [Google Scholar]
  27. YOSHITOSHI Y., NAITO C., OKANIWA H., USUI M., MOGAMI T., TOMONO T. Kinetic studies on metabolism of lipoprotein lipase. J Clin Invest. 1963 May;42:707–713. doi: 10.1172/JCI104762. [DOI] [PMC free article] [PubMed] [Google Scholar]

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