Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Mar;61(3):1132–1135. doi: 10.1128/iai.61.3.1132-1135.1993

Effect of modulation of polymorphonuclear leukocyte migration with anti-CD18 antibody on pathogenesis of experimental otitis media in guinea pigs.

J Patel 1, T Chonmaitree 1, F Schmalstieg 1
PMCID: PMC302850  PMID: 8094380

Abstract

Guinea pigs were treated with anti-CD18 antibody (M8), and subsequently middle ears (ME) were infected with nontypeable Haemophilus influenzae. Forty-eight hours after infection, the ME washes of these animals had significantly lower polymorphonuclear leukocyte numbers but higher bacterial counts compared with washes of animals treated with control antibody (M11) or saline. The edema and epithelial damage of ME tissues correlated directly with polymorphonuclear leukocyte numbers and not bacterial counts.

Full text

PDF
1134

Images in this article

1134Image
on p.1134

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernstein J. M., Humbert J. R., Hliwa M. M. Oxygen consumption of middle ear and peripheral blood neutrophils in acute suppurative otitis media. Am J Otolaryngol. 1985 May-Jun;6(3):169–172. doi: 10.1016/s0196-0709(85)80080-6. [DOI] [PubMed] [Google Scholar]
  2. Brodsky L., Faden H., Bernstein J., Stanievich J., DeCastro G., Volovitz B., Ogra P. L. Arachidonic acid metabolites in middle ear effusions of children. Ann Otol Rhinol Laryngol. 1991 Jul;100(7):589–592. doi: 10.1177/000348949110000714. [DOI] [PubMed] [Google Scholar]
  3. Clark W. M., Madden K. P., Rothlein R., Zivin J. A. Reduction of central nervous system ischemic injury in rabbits using leukocyte adhesion antibody treatment. Stroke. 1991 Jul;22(7):877–883. doi: 10.1161/01.str.22.7.877. [DOI] [PubMed] [Google Scholar]
  4. Diven W. F., Glew R. H., LaMarco K. L. Hydrolase activity in acute otitis media with effusion. Ann Otol Rhinol Laryngol. 1985 Jul-Aug;94(4 Pt 1):415–418. [PubMed] [Google Scholar]
  5. Garofalo R., Faden H., Sharma S., Ogra P. L. Release of leukotriene B4 from human neutrophils after interaction with nontypeable Haemophilus influenzae. Infect Immun. 1991 Nov;59(11):4221–4226. doi: 10.1128/iai.59.11.4221-4226.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giebink G. S., Juhn S. K., Weber M. L., Le C. T. The bacteriology and cytology of chronic otitis media with effusion. Pediatr Infect Dis. 1982 Mar-Apr;1(2):98–103. doi: 10.1097/00006454-198203000-00007. [DOI] [PubMed] [Google Scholar]
  7. Jaeschke H., Farhood A., Smith C. W. Neutrophil-induced liver cell injury in endotoxin shock is a CD11b/CD18-dependent mechanism. Am J Physiol. 1991 Dec;261(6 Pt 1):G1051–G1056. doi: 10.1152/ajpgi.1991.261.6.G1051. [DOI] [PubMed] [Google Scholar]
  8. Jung T. T. Arachidonic acid metabolites in otitis media pathogenesis. Ann Otol Rhinol Laryngol Suppl. 1988 May-Jun;132:14–18. doi: 10.1177/00034894880970s305. [DOI] [PubMed] [Google Scholar]
  9. Jung T. T. Prostaglandins, leukotrienes, and other arachidonic acid metabolites in the pathogenesis of otitis media. Laryngoscope. 1988 Sep;98(9):980–993. doi: 10.1288/00005537-198809000-00013. [DOI] [PubMed] [Google Scholar]
  10. Kishimoto T. K., Larson R. S., Corbi A. L., Dustin M. L., Staunton D. E., Springer T. A. The leukocyte integrins. Adv Immunol. 1989;46:149–182. doi: 10.1016/s0065-2776(08)60653-7. [DOI] [PubMed] [Google Scholar]
  11. Lewis D. M., Schram J. L., Meadema S. J., Lim D. J. Experimental otitis media in chinchillas. Ann Otol Rhinol Laryngol Suppl. 1980 May-Jun;89(3 Pt 2):344–350. doi: 10.1177/00034894800890s381. [DOI] [PubMed] [Google Scholar]
  12. Liu Y. S., Lim D. J., Lang R. W., Birck H. G. Chronic middle ear effusions. Immunochemical and bacteriological investigations. Arch Otolaryngol. 1975 May;101(5):278–286. doi: 10.1001/archotol.1975.00780340010003. [DOI] [PubMed] [Google Scholar]
  13. Patel J., Faden H., Sharma S., Ogra P. L. Effect of respiratory syncytial virus on adherence, colonization and immunity of non-typable Haemophilus influenzae: implications for otitis media. Int J Pediatr Otorhinolaryngol. 1992 Jan;23(1):15–23. doi: 10.1016/0165-5876(92)90075-z. [DOI] [PubMed] [Google Scholar]
  14. Sawyer D. W., Donowitz G. R., Mandell G. L. Polymorphonuclear neutrophils: an effective antimicrobial force. Rev Infect Dis. 1989 Nov-Dec;11 (Suppl 7):S1532–S1544. doi: 10.1093/clinids/11.supplement_7.s1532. [DOI] [PubMed] [Google Scholar]
  15. Schmalstieg F. C. Leukocyte adherence defect. Pediatr Infect Dis J. 1988 Dec;7(12):867–872. [PubMed] [Google Scholar]
  16. Sharar S. R., Winn R. K., Murry C. E., Harlan J. M., Rice C. L. A CD18 monoclonal antibody increases the incidence and severity of subcutaneous abscess formation after high-dose Staphylococcus aureus injection in rabbits. Surgery. 1991 Aug;110(2):213–220. [PubMed] [Google Scholar]
  17. Springer T. A. Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425–434. doi: 10.1038/346425a0. [DOI] [PubMed] [Google Scholar]
  18. Thore M., Burman L. G., Holm S. E. Streptococcus pneumoniae and three species of anaerobic bacteria in experimental otitis media in guniea pigs. J Infect Dis. 1982 Jun;145(6):822–828. doi: 10.1093/infdis/145.6.822. [DOI] [PubMed] [Google Scholar]
  19. Tuomanen E. I., Saukkonen K., Sande S., Cioffe C., Wright S. D. Reduction of inflammation, tissue damage, and mortality in bacterial meningitis in rabbits treated with monoclonal antibodies against adhesion-promoting receptors of leukocytes. J Exp Med. 1989 Sep 1;170(3):959–969. doi: 10.1084/jem.170.3.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vedder N. B., Winn R. K., Rice C. L., Chi E. Y., Arfors K. E., Harlan J. M. A monoclonal antibody to the adherence-promoting leukocyte glycoprotein, CD18, reduces organ injury and improves survival from hemorrhagic shock and resuscitation in rabbits. J Clin Invest. 1988 Mar;81(3):939–944. doi: 10.1172/JCI113407. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES