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. 1994 Dec;62(12):5669–5672. doi: 10.1128/iai.62.12.5669-5672.1994

Stimulation of monocyte tissue factor expression in an in vitro model of bacterial endocarditis.

M J Bancsi 1, J Thompson 1, R M Bertina 1
PMCID: PMC303318  PMID: 7960151

Abstract

The coagulation system plays a major role in the formation of the infected endocardial vegetation in bacterial endocarditis. Since monocytes can express tissue factor (TF) on their surfaces, they are thought to be responsible for the extrinsic activation of the coagulation cascade during this disease. The present study used an in vitro model in which fibrin plates, isolated adherent monocytes, and Streptococcus sanguis were used as an analog for endocardial vegetations. Adherence to fibrin by itself was found to stimulate TF expression on the monocytes, but stimulation by S. sanguis significantly increased TF expression, which was found to be maximal at a bacterium-to-monocyte ratio of 9 or more.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bajaj M. S., Kuppuswamy M. N., Saito H., Spitzer S. G., Bajaj S. P. Cultured normal human hepatocytes do not synthesize lipoprotein-associated coagulation inhibitor: evidence that endothelium is the principal site of its synthesis. Proc Natl Acad Sci U S A. 1990 Nov;87(22):8869–8873. doi: 10.1073/pnas.87.22.8869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bom V. J., van Hinsbergh V. W., Reinalda-Poot H. H., Mohanlal R. W., Bertina R. M. Extrinsic activation of human coagulation factors IX and X on the endothelial surface. Thromb Haemost. 1991 Sep 2;66(3):283–291. [PubMed] [Google Scholar]
  3. Buiting A. G., Thompson J., Emeis J. J., Mattie H., Brommer E. J., van Furth R. Effects of tissue-type plasminogen activator (t-PA) on Streptococcus sanguis-infected endocardial vegetations in vitro. J Antimicrob Chemother. 1988 May;21(5):609–620. doi: 10.1093/jac/21.5.609. [DOI] [PubMed] [Google Scholar]
  4. Buiting A. G., Thompson J., van der Keur D., Schmal-Bauer W. C., Bertina R. M. Procoagulant activity of endocardial vegetations and blood monocytes in rabbits with Streptococcus sanguis endocarditis. Thromb Haemost. 1989 Nov 24;62(3):1029–1033. [PubMed] [Google Scholar]
  5. Drake T. A., Rodgers G. M., Sande M. A. Tissue factor is a major stimulus for vegetation formation in enterococcal endocarditis in rabbits. J Clin Invest. 1984 Jun;73(6):1750–1753. doi: 10.1172/JCI111383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edwards R. L., Rickles F. R. The role of leukocytes in the activation of blood coagulation. Semin Hematol. 1992 Jul;29(3):202–212. [PubMed] [Google Scholar]
  7. Meddens M. J., Thompson J., Mattie H., van Furth R. Role of granulocytes in the prevention and therapy of experimental Streptococcus sanguis endocarditis in rabbits. Antimicrob Agents Chemother. 1984 Feb;25(2):263–267. doi: 10.1128/aac.25.2.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nemerson Y. The tissue factor pathway of blood coagulation. Semin Hematol. 1992 Jul;29(3):170–176. [PubMed] [Google Scholar]
  9. Rana S. V., Reimers H. J., Pathikonda M. S., Bajaj S. P. Expression of tissue factor and factor VIIa/tissue factor inhibitor activity in endotoxin or phorbol ester stimulated U937 monocyte-like cells. Blood. 1988 Jan;71(1):259–262. [PubMed] [Google Scholar]
  10. Scheld W. M., Valone J. A., Sande M. A. Bacterial adherence in the pathogenesis of endocarditis. Interaction of bacterial dextran, platelets, and fibrin. J Clin Invest. 1978 May;61(5):1394–1404. doi: 10.1172/JCI109057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thörig L., Thompson J., Eulderink F., Emeis J. J., Van Furth R. Effects of monocytopenia and anticoagulation in experimental Streptococcus sanguis endocarditis. Br J Exp Pathol. 1980 Feb;61(1):108–116. [PMC free article] [PubMed] [Google Scholar]
  12. van Ginkel C. J., Thörig L., Thompson J., Oh J. I., van Aken W. G. Enhancement of generation of monocyte tissue thromboplastin by bacterial phagocytosis: possible pathway for fibrin formation on infected vegetations in bacterial endocarditis. Infect Immun. 1979 Jul;25(1):388–395. doi: 10.1128/iai.25.1.388-395.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. van Ginkel C. J., van Aken W. G., Oh J. I., Vreeken J. Stimulation of monocyte procoagulant activity by adherence to different surfaces. Br J Haematol. 1977 Sep;37(1):35–45. [PubMed] [Google Scholar]
  14. van der Meer J. W., van de Gevel J. S., Elzenga-Claassen I., van Furth R. Suspension cultures of mononuclear phagocytes in the teflon culture bag. Cell Immunol. 1979 Jan;42(1):208–212. doi: 10.1016/0008-8749(79)90236-3. [DOI] [PubMed] [Google Scholar]

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