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. 1988 Sep;82(3):1106–1112. doi: 10.1172/JCI113667

Relationship between lipoprotein lipase activity and plasma sex steroid level in obese women.

P H Iverius 1, J D Brunzell 1
PMCID: PMC303625  PMID: 3417867

Abstract

In obese women (n = 16) at their weight, fasting adipose tissue lipoprotein lipase (LPL) activity, obtained by elution with serum and heparin at 4 degrees and 37 degrees C, was inversely correlated to plasma estradiol levels (r = -0.724; P = 0.002) and (r = -0.641; P = 0.010), respectively. Furthermore, fasting postheparin plasma LPL activity during a heparin infusion, showed an even stronger inverse correlation to plasma estradiol when measured at 60 min (r = -0.815; P less than 0.001). None of the above parameters was correlated to the body mass index. Postprandial LPL activity in postheparin plasma, measured 10 min after a heparin injection, showed a strong positive correlation with plasma free testosterone (r = 0.780; P = 0.001). Neither of these parameters was correlated with the body mass index. The origin of this LPL activity is presently unknown but could conceivably represent a pool of LPL from skeletal muscle. Since it has been shown convincingly that estrogen decreases adipose tissue LPL activity in the rat, the present studies strongly suggest that estradiol is a major negative regulator of fasting adipose tissue LPL activity in women.

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Selected References

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  1. Applebaum D. M., Goldberg A. P., Pykälistö O. J., Brunzell J. D., Hazzard W. R. Effect of estrogen on post-heparin lipolytic activity. Selective decline in hepatic triglyceride lipase. J Clin Invest. 1977 Apr;59(4):601–608. doi: 10.1172/JCI108677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ashby P., Robinson D. S. Effects of insulin, glucocorticoids and adrenaline on the activity of rat adipose-tissue lipoprotein lipids. Biochem J. 1980 Apr 15;188(1):185–192. doi: 10.1042/bj1880185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bensadoun A., Marita R. A. Dibutyryl cyclic AMP decreases the rate of lipoprotein lipase synthesis in cultured adipocytes. Biochim Biophys Acta. 1986 Dec 5;879(3):253–263. doi: 10.1016/0005-2760(86)90214-6. [DOI] [PubMed] [Google Scholar]
  4. Borensztajn J., Samols D. R., Rubenstein A. H. Effects of insulin on lipoprotein lipase activity in the rat heart and adipose tissue. Am J Physiol. 1972 Dec;223(6):1271–1275. doi: 10.1152/ajplegacy.1972.223.6.1271. [DOI] [PubMed] [Google Scholar]
  5. Bourdeaux A. M., Giudicelli Y., Rebourcet M. C., Nordmann J., Nordmann R. Influence of some gastrointestinal hormones on adipose tissue lipoprotein lipase activity in vitro: evidence of a stimulatory effect of pancreozymin and gastrin. Biochem Biophys Res Commun. 1980 Jul 16;95(1):212–219. doi: 10.1016/0006-291x(80)90726-3. [DOI] [PubMed] [Google Scholar]
  6. Bourdeaux A. M., Nordmann R., Giudicelli Y. Stimulatory effects of adenosine, adenosine analogs and insulin on adipose tissue lipoprotein lipase activity and their prevention by phosphodiesterase inhibitors. FEBS Lett. 1984 Dec 3;178(1):132–136. doi: 10.1016/0014-5793(84)81256-9. [DOI] [PubMed] [Google Scholar]
  7. Brunzell J. D., Chait A., Nikkilä E. A., Ehnholm C., Huttunen J. K., Steiner G. Heterogeneity of primary lipoprotein lipase deficiency. Metabolism. 1980 Jul;29(7):624–629. doi: 10.1016/0026-0495(80)90106-7. [DOI] [PubMed] [Google Scholar]
  8. Dark J., Wade G. N., Zucker I. Ovarian modulation of lipoprotein lipase activity in white adipose tissue of ground squirrels. Physiol Behav. 1984 Jan;32(1):75–78. doi: 10.1016/0031-9384(84)90073-8. [DOI] [PubMed] [Google Scholar]
  9. Ehnholm C., Huttunen J. K., Kinnunen P. J., Miettinen T. A., Nikkilä E. A. Effect of oxandrolone treatment on the activity of lipoprotein lipase, hepatic lipase and phospholipase A1 of human postheparin plasma. N Engl J Med. 1975 Jun 19;292(25):1314–1317. doi: 10.1056/NEJM197506192922503. [DOI] [PubMed] [Google Scholar]
  10. Glueck C. J., Gartside P., Fallat R. W., Mendoza S. Effect of sex hormones on protamine inactivated and resistant postheparin plasma lipases. Metabolism. 1976 Jun;25(6):625–632. doi: 10.1016/0026-0495(76)90059-7. [DOI] [PubMed] [Google Scholar]
  11. Gray J. M., Nunez A. A., Siegel L. I., Wade G. N. Effects of testosterone on body weight and adipose tissue: role of aromatization. Physiol Behav. 1979 Sep;23(3):465–469. doi: 10.1016/0031-9384(79)90045-3. [DOI] [PubMed] [Google Scholar]
  12. Gray J. M., Wade G. N. Cytoplasmic estrogen, but not progestin, binding sites in male rat adipose tissues. Am J Physiol. 1980 Oct;239(4):E237–E241. doi: 10.1152/ajpendo.1980.239.4.E237. [DOI] [PubMed] [Google Scholar]
  13. Hamosh M., Hamosh P. The effect of estrogen on the lipoprotein lipase activity of rat adipose tissue. J Clin Invest. 1975 May;55(5):1132–1135. doi: 10.1172/JCI108015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Heiss G., Tamir I., Davis C. E., Tyroler H. A., Rifkand B. M., Schonfeld G., Jacobs D., Frantz I. D., Jr Lipoprotein-cholesterol distributions in selected North American populations: the lipid research clinics program prevalence study. Circulation. 1980 Feb;61(2):302–315. doi: 10.1161/01.cir.61.2.302. [DOI] [PubMed] [Google Scholar]
  15. Huttunen J. K., Ehnholm C., Kinnunen P. K., Nikkilä E. A. An immunochemical method for the selective measurement of two triglyceride lipases in human postheparin plasma. Clin Chim Acta. 1975 Sep 16;63(3):335–347. doi: 10.1016/0009-8981(75)90055-8. [DOI] [PubMed] [Google Scholar]
  16. Iverius P. H., Brunzell J. D. Human adipose tissue lipoprotein lipase: changes with feeding and relation to postheparin plasma enzyme. Am J Physiol. 1985 Jul;249(1 Pt 1):E107–E114. doi: 10.1152/ajpendo.1985.249.1.E107. [DOI] [PubMed] [Google Scholar]
  17. Iverius P. H., Ostlund-Lindqvist A. M. Preparation, characterization, and measurement of lipoprotein lipase. Methods Enzymol. 1986;129:691–704. doi: 10.1016/0076-6879(86)29099-0. [DOI] [PubMed] [Google Scholar]
  18. Judd H. L., Lucas W. E., Yen S. S. Serum 17 beta-estradiol and estrone levels in postmenopausal women with and without endometrial cancer. J Clin Endocrinol Metab. 1976 Aug;43(2):272–278. doi: 10.1210/jcem-43-2-272. [DOI] [PubMed] [Google Scholar]
  19. Kim H. J., Kalkhoff R. K. Sex steroid influence on triglyceride metabolism. J Clin Invest. 1975 Oct;56(4):888–896. doi: 10.1172/JCI108168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kinnunen P. K., Unnérus H. A., Ranta T., Ehnholm C., Nikkilä E. A., Seppälä M. Activities of post-heparin plasma lipoprotein lipase and hepatic lipase during pregnancy and lactation. Eur J Clin Invest. 1980 Dec;10(6):469–474. doi: 10.1111/j.1365-2362.1980.tb02087.x. [DOI] [PubMed] [Google Scholar]
  21. Kinouchi T., Pages L., Horton R. A specific radioimmunossay for testosterone in peripheral plasma. J Lab Clin Med. 1973 Aug;82(2):309–316. [PubMed] [Google Scholar]
  22. Lehtonen A., Grönroos M., Marniemi J., Peltonen P., Mäntylä M., Niskanen J., Rautio A., Hietanen E. Effects of high dose progestin on serum lipids and lipid metabolizing enzymes in patients with endometrial cancer. Horm Metab Res. 1985 Jan;17(1):32–34. doi: 10.1055/s-2007-1013440. [DOI] [PubMed] [Google Scholar]
  23. Leuven J. A., Havekes L., van der Kooij-Pontier H. A., Starmans R. J., Jansen H., Bouwhuis-Hoogerwerf M. L., de Pagter H. A., Hessel L. W. Effect of low-dose oral contraceptives on lipoproteins and lipolytic enzymes: differences between two commonly used preparations. Metabolism. 1984 Nov;33(11):1039–1042. doi: 10.1016/0026-0495(84)90234-8. [DOI] [PubMed] [Google Scholar]
  24. Lithell H., Boberg J., Hellsing K., Lundqvist G., Vessby B. Lipoprotein-lipase activity in human skeletal muscle and adipose tissue in the fasting and the fed states. Atherosclerosis. 1978 May;30(1):89–94. doi: 10.1016/0021-9150(78)90155-7. [DOI] [PubMed] [Google Scholar]
  25. Lithell H., Boberg J. The lipoprotein-lipase activity of adipose tissue from different sites in obese women and relationship to cell size. Int J Obes. 1978;2(1):47–52. [PubMed] [Google Scholar]
  26. Lithell H., Nillius S. J., Bergh T., Selinus I. Metabolic profile in obese women with the polycystic ovary syndrome. Int J Obes. 1987;11(1):1–8. [PubMed] [Google Scholar]
  27. Longcope C., Kato T., Horton R. Conversion of blood androgens to estrogens in normal adult men and women. J Clin Invest. 1969 Dec;48(12):2191–2201. doi: 10.1172/JCI106185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Mendoza S. G., Zerpa A., Carrasco H., Colmenares O., Rangel A., Gartside P. S., Kashyap M. L. Estradiol, testosterone, apolipoproteins, lipoprotein cholesterol, and lipolytic enzymes in men with premature myocardial infarction and angiographically assessed coronary occlusion. Artery. 1983;12(1):1–23. [PubMed] [Google Scholar]
  29. Nilsson-Ehle P., Garfinkel A. S., Schotz M. C. Lipolytic enzymes and plasma lipoprotein metabolism. Annu Rev Biochem. 1980;49:667–693. doi: 10.1146/annurev.bi.49.070180.003315. [DOI] [PubMed] [Google Scholar]
  30. Patten R. L. The reciprocal regulation of lipoprotein lipase activity and hormone-sensitive lipase activity in rat adipocytes. J Biol Chem. 1970 Nov 10;245(21):5577–5584. [PubMed] [Google Scholar]
  31. Powers M. L., Florini J. R. A direct effect of testosterone on muscle cells in tissue culture. Endocrinology. 1975 Oct;97(4):1043–1047. doi: 10.1210/endo-97-4-1043. [DOI] [PubMed] [Google Scholar]
  32. Ramirez I. Estradiol-induced changes in lipoprotein lipase, eating, and body weight in rats. Am J Physiol. 1981 May;240(5):E533–E538. doi: 10.1152/ajpendo.1981.240.5.E533. [DOI] [PubMed] [Google Scholar]
  33. Rebuffe-Scrive M., Basdevant A., Guy-Grand B. Effect of local application of progesterone on human adipose tissue lipoprotein lipase. Horm Metab Res. 1983 Nov;15(11):566–566. doi: 10.1055/s-2007-1018791. [DOI] [PubMed] [Google Scholar]
  34. Rebuffé-Scrive M., Enk L., Crona N., Lönnroth P., Abrahamsson L., Smith U., Björntorp P. Fat cell metabolism in different regions in women. Effect of menstrual cycle, pregnancy, and lactation. J Clin Invest. 1985 Jun;75(6):1973–1976. doi: 10.1172/JCI111914. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Rebuffé-Scrive M., Lönnroth P., Mårin P., Wesslau C., Björntorp P., Smith U. Regional adipose tissue metabolism in men and postmenopausal women. Int J Obes. 1987;11(4):347–355. [PubMed] [Google Scholar]
  36. Reitman J. S., Kosmakos F. C., Howard B. V., Taskinen M. R., Kuusi T., Nikkila E. A. Characterization of lipase activities in obese Pima indians. Decreases with weight reduction. J Clin Invest. 1982 Oct;70(4):791–797. doi: 10.1172/JCI110675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Sauar J., Stokke K. T. Plasma estrogens and hepatic lipase in postheparin plasma with special reference to liver disease. Clin Chim Acta. 1979 Feb 15;92(1):101–105. doi: 10.1016/0009-8981(79)90402-9. [DOI] [PubMed] [Google Scholar]
  38. Semb H., Peterson J., Tavernier J., Olivecrona T. Multiple effects of tumor necrosis factor on lipoprotein lipase in vivo. J Biol Chem. 1987 Jun 15;262(17):8390–8394. [PubMed] [Google Scholar]
  39. Sjöström L., Björntorp P., Vrána J. Microscopic fat cell size measurements on frozen-cut adipose tissue in comparison with automatic determinations of osmium-fixed fat cells. J Lipid Res. 1971 Sep;12(5):521–530. [PubMed] [Google Scholar]
  40. Sorva R., Kuusi T., Dunkel L., Taskinen M. R. Effects of endogenous sex steroids on serum lipoproteins and postheparin plasma lipolytic enzymes. J Clin Endocrinol Metab. 1988 Feb;66(2):408–413. doi: 10.1210/jcem-66-2-408. [DOI] [PubMed] [Google Scholar]
  41. Speake B. K., Parkin S. M., Robinson D. S. Regulation of the synthesis of lipoprotein lipase in adipose tissue by dexamethasone. Biochim Biophys Acta. 1986 Mar 19;881(1):155–157. doi: 10.1016/0304-4165(86)90109-1. [DOI] [PubMed] [Google Scholar]
  42. Tikkanen M. J., Kuusi T., Nikkilä E. A., Sane T. Very low density lipoprotein triglyceride kinetics during hepatic lipase suppression by estrogen. Studies on the physiological role of hepatic endothelial lipase. FEBS Lett. 1985 Feb 11;181(1):160–164. doi: 10.1016/0014-5793(85)81134-0. [DOI] [PubMed] [Google Scholar]
  43. Tikkanen M. J., Kuusi T., Nikkilä E. A., Stenman U. H. Variation of postheparin plasma hepatic lipase by menstrual cycle. Metabolism. 1986 Feb;35(2):99–104. doi: 10.1016/0026-0495(86)90107-1. [DOI] [PubMed] [Google Scholar]
  44. Tikkanen M. J., Kuusi T., Vartiainen E., Nikkilä E. A. Treatment of post-menopausal hypercholesterolaemia with estradiol. Acta Obstet Gynecol Scand Suppl. 1979;88:83–88. doi: 10.3109/00016347909157234. [DOI] [PubMed] [Google Scholar]
  45. Tikkanen M. J., Nikkilä E. A., Kuusi T., Sipinen S. U. High density lipoprotein-2 and hepatic lipase: reciprocal changes produced by estrogen and norgestrel. J Clin Endocrinol Metab. 1982 Jun;54(6):1113–1117. doi: 10.1210/jcem-54-6-1113. [DOI] [PubMed] [Google Scholar]
  46. Tikkanen M. J., Nikkilä E. A., Kuusi T., Sipinen S. Effects of oestradiol and levonorgestrel on lipoprotein lipids and postheparin plasma lipase activities in normolipoproteinaemic women. Acta Endocrinol (Copenh) 1982 Apr;99(4):630–635. doi: 10.1530/acta.0.0990630. [DOI] [PubMed] [Google Scholar]
  47. Tikkanen M. J., Nikkilä E. A., Kuusi T., Sipinen S. Reduction of plasma high-density lipoprotein cholesterol and increase of postheparin plasma hepatic lipase activity during progestin treatment. Clin Chim Acta. 1981;115(1):63–71. doi: 10.1016/0009-8981(81)90107-8. [DOI] [PubMed] [Google Scholar]
  48. Vermeulen A., Stoïca T., Verdonck L. The apparent free testosterone concentration, an index of androgenicity. J Clin Endocrinol Metab. 1971 Nov;33(5):759–767. doi: 10.1210/jcem-33-5-759. [DOI] [PubMed] [Google Scholar]
  49. Vydelingum N., Drake R. L., Etienne J., Kissebah A. H. Insulin regulation of fat cell ribosomes, protein synthesis, and lipoprotein lipase. Am J Physiol. 1983 Aug;245(2):E121–E131. doi: 10.1152/ajpendo.1983.245.2.E121. [DOI] [PubMed] [Google Scholar]
  50. Wilson D. E., Flowers C. M., Carlile S. I., Udall K. S. Estrogen treatment and gonadal function in the regulation of lipoprotein lipase. Atherosclerosis. 1976 Sep;24(3):491–499. doi: 10.1016/0021-9150(76)90141-6. [DOI] [PubMed] [Google Scholar]

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