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. 1989 Mar;83(3):1010–1014. doi: 10.1172/JCI113942

Induction of resistance to Schistosoma mansoni infection in mice by purified parasite paramyosin.

T P Flanigan 1, C H King 1, R R Lett 1, J Nanduri 1, A A Mahmoud 1
PMCID: PMC303778  PMID: 2493482

Abstract

Freeze-thaw (FT)-disrupted schistosomula or their membrane extract induced significant resistance in mice to Schistosoma mansoni infection (34 and 25%, respectively) without the use of adjuvant. Antigens identified in schistosome extracts by sera from immunized animals were then evaluated for protective potential. Immunization with schistosomal antigens of 97 and 68-70 kD resulted in significant protection that was equivalent to that obtained by FT schistosomula. Since the 97-kD antigen was suggested to be parasite paramyosin, we used a biochemical technique to purify this muscle protein. Purified schistosome paramyosin ran as a single band on 10% SDS-PAGE and was recognized both by sera from mice immunized with FT schistosomula and a polyclonal antiserum raised against the 97-kD parasite protein. Preincubation of schistosome paramyosin with sera from mice immunized with FT schistosomula resulted in the removal of reactivity with the 97-kD protein in crude worm extracts. Paramyosin was identified by Western blotting to be in the tegument of schistosomula. The purified schistosome paramyosin resulted in significant protection in three separate experiments (24, 46, and 53%) without the use of adjuvant. Addition of BCG to paramyosin resulted in enhanced protection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balloul J. M., Sondermeyer P., Dreyer D., Capron M., Grzych J. M., Pierce R. J., Carvallo D., Lecocq J. P., Capron A. Molecular cloning of a protective antigen of schistosomes. Nature. 1987 Mar 12;326(6109):149–153. doi: 10.1038/326149a0. [DOI] [PubMed] [Google Scholar]
  2. Butterworth A. E., Taylor D. W., Veith M. C., Vadas M. A., Dessein A., Sturrock R. F., Wells E. Studies on the mechanisms of immunity in human schistosomiasis. Immunol Rev. 1982;61:5–39. doi: 10.1111/j.1600-065x.1982.tb00372.x. [DOI] [PubMed] [Google Scholar]
  3. Clegg J. A., Smithers S. R. The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. Int J Parasitol. 1972 Mar;2(1):79–98. doi: 10.1016/0020-7519(72)90036-7. [DOI] [PubMed] [Google Scholar]
  4. Cohen C. Matching molecules in the catch mechanism. Proc Natl Acad Sci U S A. 1982 May;79(10):3176–3178. doi: 10.1073/pnas.79.10.3176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Colley D. G., Cook J. A., Freeman G. L., Jr, Bartholomew R. K., Jordan P. Immune responses during human schistosomiasis mansoni. I. In vitro lymphocyte blastogenic responses to heterogeneous antigenic preparations from schistosome eggs, worms and cercariae. Int Arch Allergy Appl Immunol. 1977;53(5):420–433. [PubMed] [Google Scholar]
  6. Harris H. E., Epstein H. F. Myosin and paramyosin of Caenorhabditis elegans: biochemical and structural properties of wild-type and mutant proteins. Cell. 1977 Apr;10(4):709–719. doi: 10.1016/0092-8674(77)90105-2. [DOI] [PubMed] [Google Scholar]
  7. James S. L., Pearce E. J., Sher A. Induction of protective immunity against Schistosoma mansoni by a non living vaccine. I. Partial characterization of antigens recognized by antibodies from mice immunized with soluble schistosome extracts. J Immunol. 1985 May;134(5):3432–3438. [PubMed] [Google Scholar]
  8. King C. H., Lett R. R., Nanduri J., el Ibiary S., Peters P. A., Olds G. R., Mahmoud A. A. Isolation and characterization of a protective antigen for adjuvant-free immunization against Schistosoma mansoni. J Immunol. 1987 Dec 15;139(12):4218–4224. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Lanar D. E., Pearce E. J., James S. L., Sher A. Identification of paramyosin as schistosome antigen recognized by intradermally vaccinated mice. Science. 1986 Oct 31;234(4776):593–596. doi: 10.1126/science.3094144. [DOI] [PubMed] [Google Scholar]
  11. Lustigman S., Mahmoud A. A., Hamburger J. Glycopeptides in soluble egg antigen of Schistosoma mansoni: isolation, characterization, and elucidation of their immunochemical and immunopathological relation to the major egg glycoprotein (MEG). J Immunol. 1985 Mar;134(3):1961–1967. [PubMed] [Google Scholar]
  12. Matsumoto Y., Perry G., Levine R. J., Blanton R., Mahmoud A. A., Aikawa M. Paramyosin and actin in schistosomal teguments. Nature. 1988 May 5;333(6168):76–78. doi: 10.1038/333076a0. [DOI] [PubMed] [Google Scholar]
  13. Minard P., Dean D. A., Jacobson R. H., Vannier W. E., Murrell K. D. Immunization of mice with cobalt-60 irradiated Schistosoma mansoni cercariae. Am J Trop Med Hyg. 1978 Jan;27(1 Pt 1):76–86. doi: 10.4269/ajtmh.1978.27.76. [DOI] [PubMed] [Google Scholar]
  14. Murrell K. D., Dean D. A., Stafford E. E. Resistance to infection with Schistosoma mansoni after immunization with worm extracts or live cercariae: role of cytotoxic antibody in mice and guinea pigs. Am J Trop Med Hyg. 1975 Nov;24(6 Pt 1):955–962. doi: 10.4269/ajtmh.1975.24.955. [DOI] [PubMed] [Google Scholar]
  15. Pearce E. J., James S. L., Dalton J., Barrall A., Ramos C., Strand M., Sher A. Immunochemical characterization and purification of Sm-97, a Schistosoma mansoni antigen monospecifically recognized by antibodies from mice protectively immunized with a nonliving vaccine. J Immunol. 1986 Dec 1;137(11):3593–3600. [PubMed] [Google Scholar]
  16. Pearce E. J., James S. L., Hieny S., Lanar D. E., Sher A. Induction of protective immunity against Schistosoma mansoni by vaccination with schistosome paramyosin (Sm97), a nonsurface parasite antigen. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5678–5682. doi: 10.1073/pnas.85.15.5678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ramalho-Pinto F. J., Gazzinelli G., Howells R. E., Mota-Santos T. A., Figueiredo E. A., Pellegrino J. Schistosoma mansoni: defined system for stepwise transformation of cercaria to schistosomule in vitro. Exp Parasitol. 1974 Dec;36(3):360–372. doi: 10.1016/0014-4894(74)90076-9. [DOI] [PubMed] [Google Scholar]
  18. Rocklin R. E., Brown A. P., Warren K. S., Pelley R. P., Houba V., Siongok T. K., Ouma J., Sturrock R. F., Butterworth A. E. Factors that modify the cellular-immune response in patients infected by Schistosoma mansoni. J Immunol. 1980 Nov;125(5):1916–1923. [PubMed] [Google Scholar]
  19. Sher A., Pearce E., Hieny S., James S. Induction of protective immunity against Schistosoma mansoni by a nonliving vaccine. IV. Fractionation and antigenic properties of a soluble adult worm immunoprophylactic activity. J Immunol. 1986 May 15;136(10):3878–3883. [PubMed] [Google Scholar]
  20. Smith M. A., Clegg J. A. Vaccination against Schistosoma mansoni with purified surface antigens. Science. 1985 Feb 1;227(4686):535–538. doi: 10.1126/science.3966161. [DOI] [PubMed] [Google Scholar]
  21. Smithers S. R., Terry R. J. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology. 1965 Nov;55(4):695–700. doi: 10.1017/s0031182000086248. [DOI] [PubMed] [Google Scholar]
  22. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Waterston R. H., Epstein H. F., Brenner S. Paramyosin of Caenorhabditis elegans. J Mol Biol. 1974 Dec 5;90(2):285–290. doi: 10.1016/0022-2836(74)90373-8. [DOI] [PubMed] [Google Scholar]

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