Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1987 Jan;84(1):146–150. doi: 10.1073/pnas.84.1.146

Reconstitution of chlorophyll a/b light-harvesting complexes: Xanthophyll-dependent assembly and energy transfer

F Gerald Plumley 1, Gregory W Schmidt 1
PMCID: PMC304159  PMID: 16593794

Abstract

A method for in vitro reconstitution of the chlorophyll a/b light-harvesting complex from LiDodSO4/heat-denatured or acetone-extracted photosynthetic membranes has been developed. Characterization of the minimum components necessary for the functional organization of pigments in these membrane complexes reveals that xanthophylls are essential structural components.

Keywords: thylakoids, pigments, diacylglycerides, photosynthesis, membrane biogenesis

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett J. Regulation of photosynthesis by reversible phosphorylation of the light-harvesting chlorophyll a/b protein. Biochem J. 1983 Apr 15;212(1):1–13. doi: 10.1042/bj2120001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chua N. H., Bennoun P. Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175–2179. doi: 10.1073/pnas.72.6.2175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Delepelaire P., Chua N. H. Electrophoretic purification of chlorophyll a/b-protein complexes from Chlamydomonas reinhardtii and spinach and analysis of their polypeptide compositions. J Biol Chem. 1981 Sep 10;256(17):9300–9307. [PubMed] [Google Scholar]
  4. Dunsmuir P. The petunia chlorophyll a/b binding protein genes: a comparison of Cab genes from different gene families. Nucleic Acids Res. 1985 Apr 11;13(7):2503–2518. doi: 10.1093/nar/13.7.2503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Janero D. R., Barrnett R. Thylakoid membrane biogenesis in Chlamydomonas reinhardtii 137+. II. Cell-cycle variations in the synthesis and assembly of pigment. J Cell Biol. 1982 May;93(2):411–416. doi: 10.1083/jcb.93.2.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Plumley F. G., Schmidt G. W. Rocket and crossed immunoelectrophoresis of proteins solubilized with sodium dodecyl sulfate. Anal Biochem. 1983 Oct 1;134(1):86–95. doi: 10.1016/0003-2697(83)90267-1. [DOI] [PubMed] [Google Scholar]
  8. Radunz A. Binding of antibodies onto the thylakoid membrane. IV. Phosphatides and xanthophylls in the outer surface of the thylakoid membrane. Z Naturforsch C. 1978 Nov-Dec;33(11-12):941–947. doi: 10.1515/znc-1978-11-1223. [DOI] [PubMed] [Google Scholar]
  9. Schmidt G. W., Bartlett S. G., Grossman A. R., Cashmore A. R., Chua N. H. Biosynthetic pathways of two polypeptide subunits of the light-harvesting chlorophyll a/b protein complex. J Cell Biol. 1981 Nov;91(2 Pt 1):468–478. doi: 10.1083/jcb.91.2.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schmidt G. W., Mishkind M. L. Rapid degradation of unassembled ribulose 1,5-bisphosphate carboxylase small subunits in chloroplasts. Proc Natl Acad Sci U S A. 1983 May;80(9):2632–2636. doi: 10.1073/pnas.80.9.2632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schmidt G. W., Mishkind M. L. The transport of proteins into chloroplasts. Annu Rev Biochem. 1986;55:879–912. doi: 10.1146/annurev.bi.55.070186.004311. [DOI] [PubMed] [Google Scholar]
  12. Sprague S. G., Camm E. L., Green B. R., Staehelin L. A. Reconstitution of light-harvesting complexes and photosystem II cores into galactolipid and phospholipid liposomes. J Cell Biol. 1985 Feb;100(2):552–557. doi: 10.1083/jcb.100.2.552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Van Metter R. L. Excitation energy transfer in the light-harvesting chlorophyll a/b.protein. Biochim Biophys Acta. 1977 Dec 23;462(3):642–658. doi: 10.1016/0005-2728(77)90107-4. [DOI] [PubMed] [Google Scholar]
  14. Yamamoto M., Kosuda T., Yanagawa H., Tachibana T., Shima K., Hosoda Y., Mikami R., Homma H. Long-term follow-up in sarcoidosis in Japan. Z Erkr Atmungsorgane. 1977 Aug;149(2):191–196. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES