Abstract
A method for in vitro reconstitution of the chlorophyll a/b light-harvesting complex from LiDodSO4/heat-denatured or acetone-extracted photosynthetic membranes has been developed. Characterization of the minimum components necessary for the functional organization of pigments in these membrane complexes reveals that xanthophylls are essential structural components.
Keywords: thylakoids, pigments, diacylglycerides, photosynthesis, membrane biogenesis
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bennett J. Regulation of photosynthesis by reversible phosphorylation of the light-harvesting chlorophyll a/b protein. Biochem J. 1983 Apr 15;212(1):1–13. doi: 10.1042/bj2120001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chua N. H., Bennoun P. Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175–2179. doi: 10.1073/pnas.72.6.2175. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Delepelaire P., Chua N. H. Electrophoretic purification of chlorophyll a/b-protein complexes from Chlamydomonas reinhardtii and spinach and analysis of their polypeptide compositions. J Biol Chem. 1981 Sep 10;256(17):9300–9307. [PubMed] [Google Scholar]
- Dunsmuir P. The petunia chlorophyll a/b binding protein genes: a comparison of Cab genes from different gene families. Nucleic Acids Res. 1985 Apr 11;13(7):2503–2518. doi: 10.1093/nar/13.7.2503. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janero D. R., Barrnett R. Thylakoid membrane biogenesis in Chlamydomonas reinhardtii 137+. II. Cell-cycle variations in the synthesis and assembly of pigment. J Cell Biol. 1982 May;93(2):411–416. doi: 10.1083/jcb.93.2.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Plumley F. G., Schmidt G. W. Rocket and crossed immunoelectrophoresis of proteins solubilized with sodium dodecyl sulfate. Anal Biochem. 1983 Oct 1;134(1):86–95. doi: 10.1016/0003-2697(83)90267-1. [DOI] [PubMed] [Google Scholar]
- Radunz A. Binding of antibodies onto the thylakoid membrane. IV. Phosphatides and xanthophylls in the outer surface of the thylakoid membrane. Z Naturforsch C. 1978 Nov-Dec;33(11-12):941–947. doi: 10.1515/znc-1978-11-1223. [DOI] [PubMed] [Google Scholar]
- Schmidt G. W., Bartlett S. G., Grossman A. R., Cashmore A. R., Chua N. H. Biosynthetic pathways of two polypeptide subunits of the light-harvesting chlorophyll a/b protein complex. J Cell Biol. 1981 Nov;91(2 Pt 1):468–478. doi: 10.1083/jcb.91.2.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt G. W., Mishkind M. L. Rapid degradation of unassembled ribulose 1,5-bisphosphate carboxylase small subunits in chloroplasts. Proc Natl Acad Sci U S A. 1983 May;80(9):2632–2636. doi: 10.1073/pnas.80.9.2632. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt G. W., Mishkind M. L. The transport of proteins into chloroplasts. Annu Rev Biochem. 1986;55:879–912. doi: 10.1146/annurev.bi.55.070186.004311. [DOI] [PubMed] [Google Scholar]
- Sprague S. G., Camm E. L., Green B. R., Staehelin L. A. Reconstitution of light-harvesting complexes and photosystem II cores into galactolipid and phospholipid liposomes. J Cell Biol. 1985 Feb;100(2):552–557. doi: 10.1083/jcb.100.2.552. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Metter R. L. Excitation energy transfer in the light-harvesting chlorophyll a/b.protein. Biochim Biophys Acta. 1977 Dec 23;462(3):642–658. doi: 10.1016/0005-2728(77)90107-4. [DOI] [PubMed] [Google Scholar]
- Yamamoto M., Kosuda T., Yanagawa H., Tachibana T., Shima K., Hosoda Y., Mikami R., Homma H. Long-term follow-up in sarcoidosis in Japan. Z Erkr Atmungsorgane. 1977 Aug;149(2):191–196. [PubMed] [Google Scholar]