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. 1987 Jan;84(1):214–218. doi: 10.1073/pnas.84.1.214

Chimeric antibody with human constant regions and mouse variable regions directed against carcinoma-associated antigen 17-1A.

L K Sun, P Curtis, E Rakowicz-Szulczynska, J Ghrayeb, N Chang, S L Morrison, H Koprowski
PMCID: PMC304173  PMID: 3025856

Abstract

We have cloned the genomic DNA fragments encoding the heavy and light chain variable regions of monoclonal antibody 17-1A, and we have inserted them into mammalian expression vectors containing genomic DNA segments encoding human gamma 3 and kappa constant regions. The transfer of these expression vectors containing mouse-human chimeric immunoglobulin genes into Sp2/0 mouse myeloma cells resulted in the production of functional IgG that retained the specific binding to the surface antigen 17-1A expressed on colorectal carcinoma cells.

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Selected References

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