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. 1987 Apr;84(7):1754–1758. doi: 10.1073/pnas.84.7.1754

The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication.

T M Hill, J M Henson, P L Kuempel
PMCID: PMC304519  PMID: 3550796

Abstract

The terminus region of the chromosome of Escherichia coli contains two separate sites, called T1 and T2, that inhibit replication forks. T1 is located near 28.5 min, which is adjacent to trp, and T2 is located at 34.5-35.7 min on the opposite side of the terminus region, near manA. The sites act in a polar fashion, and replication forks traveling in a clockwise direction with respect to the genetic map are not inhibited as they pass through T1 but are inhibited at T2. Similarly, counterclockwise forks are not inhibited at T2 but are inhibited at T1. Consequently, forks are not inhibited until they have passed through the terminus region and are about to leave it. Studies with deletion strains have located T2 within a 58-kilobase interval, which corresponds to kilobase coordinates 387-445 on the physical map of the terminus region.

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Selected References

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  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bertani L. E., Bertani G. Preparation and characterization of temperate, non-inducible bacteriophage P2 (host: Escherichia coli). J Gen Virol. 1970 Feb;6(2):201–212. doi: 10.1099/0022-1317-6-2-201. [DOI] [PubMed] [Google Scholar]
  3. Binding R., Romansky G., Bitner R., Kuempel P. Isolation and properties of Tn10 insertions in the rac locus of Escherichia coli. Mol Gen Genet. 1981;183(2):333–340. doi: 10.1007/BF00270637. [DOI] [PubMed] [Google Scholar]
  4. Bochner B. R., Huang H. C., Schieven G. L., Ames B. N. Positive selection for loss of tetracycline resistance. J Bacteriol. 1980 Aug;143(2):926–933. doi: 10.1128/jb.143.2.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bouché J. P., Gélugne J. P., Louarn J., Louarn J. M., Kaiser K. Relationships between the physical and genetic maps of a 470 x 10(3) base-pair region around the terminus of Escherichia coli K12 DNA replication. J Mol Biol. 1982 Jan 5;154(1):21–32. doi: 10.1016/0022-2836(82)90414-4. [DOI] [PubMed] [Google Scholar]
  6. Béjar S., Bouché J. P. Molecular cloning of the region of the terminus of Escherichia coli K-12 DNA replication. J Bacteriol. 1983 Feb;153(2):604–609. doi: 10.1128/jb.153.2.604-609.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clarke L., Carbon J. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell. 1976 Sep;9(1):91–99. doi: 10.1016/0092-8674(76)90055-6. [DOI] [PubMed] [Google Scholar]
  8. Diaz R., Barnsley P., Pritchard R. H. Location and characterisation of a new replication origin in the E. coli K12 chromosome. Mol Gen Genet. 1979 Sep;175(2):151–157. doi: 10.1007/BF00425531. [DOI] [PubMed] [Google Scholar]
  9. Eisenbeis S. J., Parker J. Strains of Escherichia coli carrying the structural gene for histidyl-tRNA synthetase on a high copy-number plasmid. Mol Gen Genet. 1981;183(1):115–122. doi: 10.1007/BF00270148. [DOI] [PubMed] [Google Scholar]
  10. Henson J. M., Kuempel P. L. Deletion of the terminus region (340 kilobase pairs of DNA) from the chromosome of Escherichia coli. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3766–3770. doi: 10.1073/pnas.82.11.3766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Henson J. M., Kuempel P. L. The use of transposon insertion zdc-235::Tn10 (min 32) to clone and delete DNA from the terminus region of Escherichia coli. Mol Gen Genet. 1983;189(3):506–512. doi: 10.1007/BF00325918. [DOI] [PubMed] [Google Scholar]
  12. Kolter R., Helinski D. R. Activity of the replication terminus of plasmid R6K in hybrid replicons in Escherichia coli. J Mol Biol. 1978 Sep 25;124(3):425–441. doi: 10.1016/0022-2836(78)90180-8. [DOI] [PubMed] [Google Scholar]
  13. Kuempel P. L., Duerr S. A. Chromosome replication in Escherichia coli is inhibited in the terminus region near the rac locus. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):563–567. doi: 10.1101/sqb.1979.043.01.062. [DOI] [PubMed] [Google Scholar]
  14. Kuempel P. L., Duerr S. A., Seeley N. R. Terminus region of the chromosome in Escherichia coli inhibits replication forks. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3927–3931. doi: 10.1073/pnas.74.9.3927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kuempel P. L., Maglothin P., Prescott D. M. Bidirectional termination of chromosome replication in Escherichia coli. Mol Gen Genet. 1973 Sep 5;125(1):1–8. doi: 10.1007/BF00292981. [DOI] [PubMed] [Google Scholar]
  16. Louarn J., Bouché J. P., Patte J., Louarn J. M. Genetic inactivation of topoisomerase I suppresses a defect in initiation of chromosome replication in Escherichia coli. Mol Gen Genet. 1984;195(1-2):170–174. doi: 10.1007/BF00332741. [DOI] [PubMed] [Google Scholar]
  17. Louarn J., Patte J., Louarn J. M. Evidence for a fixed termination site of chromosome replication in Escherichia coli K12. J Mol Biol. 1977 Sep 25;115(3):295–314. doi: 10.1016/0022-2836(77)90156-5. [DOI] [PubMed] [Google Scholar]
  18. Louarn J., Patte J., Louarn J. M. Map position of the replication terminus on the Escherichia coli chromosome. Mol Gen Genet. 1979 Apr 17;172(1):7–11. doi: 10.1007/BF00276208. [DOI] [PubMed] [Google Scholar]
  19. Louarn J., Patte J., Louarn J. M. Suppression of Escherichia coli dnaA46 mutations by integration of plasmid R100.1. derivatives: constraints imposed by the replication terminus. J Bacteriol. 1982 Aug;151(2):657–667. doi: 10.1128/jb.151.2.657-667.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Monteiro M. J., Sargent M. G., Piggot P. J. Characterization of the replication terminus of the Bacillus subtilis chromosome. J Gen Microbiol. 1984 Sep;130(9):2403–2414. doi: 10.1099/00221287-130-9-2403. [DOI] [PubMed] [Google Scholar]
  21. Morris P. W., Binkley J. P., Henson J. M., Kuempel P. L. Cloning and location of the dgsA gene of Escherichia coli. J Bacteriol. 1985 Aug;163(2):785–786. doi: 10.1128/jb.163.2.785-786.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Plumbridge J. A., Howe J. G., Springer M., Touati-Schwartz D., Hershey J. W., Grunberg-Manago M. Cloning and mapping of a gene for translational initiation factor IF2 in Escherichia coli. Proc Natl Acad Sci U S A. 1982 Aug;79(16):5033–5037. doi: 10.1073/pnas.79.16.5033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Plumbridge J. A., Springer M., Graffe M., Goursot R., Grunberg-Manago M. Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation. Gene. 1980 Oct;11(1-2):33–42. doi: 10.1016/0378-1119(80)90084-0. [DOI] [PubMed] [Google Scholar]
  24. Roehl R. A., Vinopal R. T. Genetic locus, distant from ptsM, affecting enzyme IIA/IIB function in Escherichia coli K-12. J Bacteriol. 1980 Apr;142(1):120–130. doi: 10.1128/jb.142.1.120-130.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sancar A., Wharton R. P., Seltzer S., Kacinski B. M., Clarke N. D., Rupp W. D. Identification of the uvrA gene product. J Mol Biol. 1981 May 5;148(1):45–62. doi: 10.1016/0022-2836(81)90234-5. [DOI] [PubMed] [Google Scholar]
  26. Shapira S. K., Chou J., Richaud F. V., Casadaban M. J. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase. Gene. 1983 Nov;25(1):71–82. doi: 10.1016/0378-1119(83)90169-5. [DOI] [PubMed] [Google Scholar]
  27. Shaw D. J., Guest J. R. Molecular cloning of the fnr gene of Escherichia coli K12. Mol Gen Genet. 1981;181(1):95–100. doi: 10.1007/BF00339011. [DOI] [PubMed] [Google Scholar]
  28. Takeda Y., Nishimura A., Nishimura Y., Yamada M., Yasuda S., Suzuki H., Hirota Y. Synthetic ColE1 Plasmids carrying genes for penicillin-binding proteins in Escherichia coli. Plasmid. 1981 Jul;6(1):86–98. doi: 10.1016/0147-619x(81)90056-1. [DOI] [PubMed] [Google Scholar]
  29. Weiss A. S., Wake R. G. A unique DNA intermediate associated with termination of chromosome replication in Bacillus subtilis. Cell. 1984 Dec;39(3 Pt 2):683–689. doi: 10.1016/0092-8674(84)90475-6. [DOI] [PubMed] [Google Scholar]
  30. Weiss A. S., Wake R. G. Impediment to replication fork movement in the terminus region of the Bacillus subtilis chromosome. J Mol Biol. 1984 Nov 15;179(4):745–750. doi: 10.1016/0022-2836(84)90165-7. [DOI] [PubMed] [Google Scholar]
  31. Weiss A. S., Wake R. G. Restriction map of DNA spanning the replication terminus of the Bacillus subtilis chromosome. J Mol Biol. 1983 Dec 5;171(2):119–137. doi: 10.1016/s0022-2836(83)80349-0. [DOI] [PubMed] [Google Scholar]
  32. de Massy B., Béjar S., Louarn J., Louarn J. M., Bouché J. P. Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1759–1763. doi: 10.1073/pnas.84.7.1759. [DOI] [PMC free article] [PubMed] [Google Scholar]

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